Figure 2.

WT-TTR and M-TTR display strong inhibition of primary nucleation in Aβ₄₀ aggregation. (a–f) Time courses of fibril formation by 5.0 μM Aβ₄₀ in the presence of WT-TTR (a–c) and M-TTR (d–f) at the indicated concentrations (m.e.). The solid lines are fits of the kinetic profiles by a model in which secondary nucleation (a, d), elongation (b, e), or primary nucleation (c, f) is inhibited by TTRs. (g) Incubation of 20, 30, and 50 μM WT-TTR and M-TTR under the same conditions in the absence of Aβ₄₀. (h) Change in the effective rate constants of primary nucleation in Aβ₄₀ aggregation, as derived from (e) and (f), shown with increasing concentrations of WT-TTR (green) and M-TTR (black). Error bars are s.d. of the rates obtained from individual fitting of the repeats. The traces in (a)–(f) show the average traces of those repeats.