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. 2021 Mar 17;10:e63886. doi: 10.7554/eLife.63886

A KDM5–Prospero transcriptional axis functions during early neurodevelopment to regulate mushroom body formation

Hayden AM Hatch 1, Helen M Belalcazar 2, Owen J Marshall 3, Julie Secombe 1,2,
Editors: Laura A Bradfield4, K VijayRaghavan5
PMCID: PMC7997662  PMID: 33729157

Abstract

Mutations in the lysine demethylase 5 (KDM5) family of transcriptional regulators are associated with intellectual disability, yet little is known regarding their spatiotemporal requirements or neurodevelopmental contributions. Utilizing the mushroom body (MB), a major learning and memory center within the Drosophila brain, we demonstrate that KDM5 is required within ganglion mother cells and immature neurons for proper axogenesis. Moreover, the mechanism by which KDM5 functions in this context is independent of its canonical histone demethylase activity. Using in vivo transcriptional and binding analyses, we identify a network of genes directly regulated by KDM5 that are critical modulators of neurodevelopment. We find that KDM5 directly regulates the expression of prospero, a transcription factor that we demonstrate is essential for MB morphogenesis. Prospero functions downstream of KDM5 and binds to approximately half of KDM5-regulated genes. Together, our data provide evidence for a KDM5–Prospero transcriptional axis that is essential for proper MB development.

Research organism: D. melanogaster

Introduction

Intellectual disability (ID) is reported to affect 1.5–3% of the global population and represents a class of neurodevelopmental disorders characterized by cognitive impairments that result in lifelong educational, social, and financial consequences for patients and their caregivers (van Bokhoven, 2011; Leonard and Wen, 2002). ID disorders are diagnosed during early childhood and are defined by an IQ score of less than 70 with deficits in adaptive behaviors (Ropers, 2010). However, despite these profound burdens, little is known regarding the pathogenesis of ID disorders, particularly how disruptions in genetic and neuronal regulatory programs contribute to cognitive and behavioral dysfunction.

Advances in comparative genomic hybridization and whole-exome sequencing have revealed strong associations between ID and mutations in genes encoding chromatin-modifying enzymes (Elbert and Bérubé, 2014; Harmeyer et al., 2017; Kong et al., 2018; Parkel et al., 2013). These proteins post-translationally modify chromatin by inserting or removing chemical moieties from histone tails to regulate DNA accessibility or alter the recruitment proteins needed to modulate transcriptional initiation or elongation (van Bokhoven, 2011; Liefke et al., 2010; Liu et al., 2014; van Oevelen et al., 2008; Secombe et al., 2007; Vallianatos and Iwase, 2015). One class of ID-associated chromatin modifiers is the lysine demethylase 5 (KDM5) family of transcriptional regulators, with mammals encoding four KDM5 paralogs: KDM5A, KDM5B, KDM5C, and KDM5D. Loss-of-function mutations in KDM5A, KDM5B, and KDM5C are associated with ID, with genetic variants in KDM5C associated with a disorder known as mental retardation, X-linked, syndromic, Claes–Jensen type (MRXSCJ, OMIM# 300534).key. 

The generation of KDM5 knockout animal models has greatly assisted in our ability to investigate the neuromorphological and behavioral consequences of KDM5 loss of function. Previous in vitro studies examining rat cerebellar granular neurons and pyramidal neurons of prepared mouse basolateral amygdala slices demonstrate that Kdm5c knockout results in dendritic spine abnormalities (Iwase et al., 2016). Similarly, loss-of-function mutations in rbr-2, the sole Caenorhabditis elegans Kdm5c ortholog, result in axonal growth and guidance defects (Mariani et al., 2016). Additionally, Kdm5c knockout mice display behavioral deficits that are analogous to those exhibited by patients with pathogenic KDM5C variants, such as increased aggression, learning and memory impairments, and decreased seizure thresholds (Iwase et al., 2016; Scandaglia et al., 2017). Together, these studies suggest that the neuromorphological and functional impairments resulting from loss of orthologous KDM5 proteins are likely to be attributed to altered gene expression within neurons.

KDM5 proteins demethylate trimethyl groups on lysine 4 of histone H3 (H3K4me3) via the enzymatic activity of their Jumonji C (JmjC) domains (Liefke et al., 2010; Liu et al., 2014; Secombe et al., 2007; van Oevelen et al., 2008). High levels of H3K4me3 near transcriptional start sites (TSS) are associated with actively transcribed genes, suggesting that KDM5 proteins can dynamically regulate transcription (Greer and Shi, 2012). Prevailing models linking alterations in KDM5 family protein function to ID suggest that loss of JmjC-mediated demethylase activity is a key driver of neuronal dysfunction (Belalcazar et al., 2021; Mariani et al., 2016; Scandaglia et al., 2017; Vallianatos and Iwase, 2015; Vallianatos et al., 2020; Zamurrad et al., 2018). For example, Vallianatos and colleagues have demonstrated that the neuronal and behavioral phenotypes observed in Kdm5c knockout mice can be rescued by reducing levels of the H3K4 methyltransferase KMT2A (Vallianatos et al., 2020). Similarly, Drosophila KDM5 acts in a demethylase-dependent manner to regulate long- and short-term olfactory memory (Zamurrad et al., 2018).

KDM5 family proteins can also regulate transcription independently of their demethylase activity by associating with other chromatin-modifying proteins (Gajan et al., 2016; Lee et al., 2007; Lee et al., 2009; Nishibuchi et al., 2014). For example, the HDAC complex member SIN3A has been shown in vitro to interact with Drosophila KDM5 and regulate overlapping subsets of genes. In mice, SIN3A is required for neuronal development (Gajan et al., 2016; Witteveen et al., 2016) with mutations in SIN3A associated with Witteveen–Kolk syndrome (OMIM# 613406), a neurodevelopmental disorder characterized by developmental delay and ID (Witteveen et al., 2016). Additionally, a subset of ID-associated KDM5C missense mutations have been shown in vitro not to affect H3K4me3 demethylase activity, yet alter transcriptional outputs (Brookes et al., 2015; Vallianatos et al., 2018). Collectively, these data provide strong evidence that disruption of KDM5 protein function may impact multiple transcriptional pathways critical to neuronal development and function.

Here, we utilize Drosophila, which encodes a single, highly conserved KDM5 ortholog known as kdm5 (previously known as little imaginal discs [lid]), to investigate the genetic and neuromorphological consequences of KDM5 loss during neurodevelopment. Our analyses focus on a group of neurons known as Kenyon cells, which form a bilateral, neuropil-rich structure known as the mushroom body (MB). The MB is essential for orchestrating a diverse repertoire of cognitive processes and is thus routinely used to study neuroanatomical changes associated with mutations in ID-related genes (Androschuk et al., 2015; Aso et al., 2014; Dubnau et al., 2001; Heisenberg et al., 1985). In fact, loss-of-function mutations in orthologous genes associated with ID, such as the Fragile X syndrome gene fmr1, the Down syndrome gene dscam, and the ZC3H14 autosomal-recessive ID gene dnab2, result in severe morphological defects of the MB (Hattori et al., 2007; Kelly et al., 2016; Michel et al., 2004; Zhan et al., 2004).

The development of the MB is dependent on four MB neuroblasts (MBNBs) per hemisphere dividing asymmetrically throughout development. Each MBNB gives rise to another MBNB and a ganglion mother cell (GMC), which in turn divides symmetrically to form two Kenyon cells. Three subclasses of Kenyon cells give rise to the MB and are born in a highly regulated and sequential manner with tight temporal control. The first-born Kenyon cells are referred to as γ Kenyon cells and develop between the embryonic and mid-third-instar larval stage, giving rise to the γ lobes. The α′/β′ lobes are the next to develop, followed by the α/β lobes during the late larval and pupal stages. Notably, specification of these Kenyon cell subsets is transcriptionally regulated through the timed expression of a number of transcription factors (Bates et al., 2010; Marchetti and Tavosanis, 2017; Syed et al., 2017).

We show here that kdm5 gene knockout and shRNA-mediated depletion of kdm5 within GMCs and immature neurons both result in profound MB structural defects. Furthermore, using an in vivo transcriptomics-based approach, we identify subsets of genes within GMCs and immature Kenyon cells that are downregulated upon kdm5 depletion. One such gene, prospero (pros), encodes a homeodomain-containing transcription factor that is required for axon pathfinding and growth in other neuronal cell types; however, its importance in regulating MB development remains unexplored. We find here that MB-specific knockdown of pros leads to aberrant MB formation and demonstrate that KDM5 directly binds to and regulates pros expression within GMCs and immature neurons. As approximately half of KDM5 regulated genes are bound by Pros, we propose a model by which KDM5 regulates the expression of Pros and its targets to promote proper MB formation. Our studies thus provide the first in vivo analysis of KDM5 within a specific cell population, revealing a key kdm5-pros genetic pathway critical for neurodevelopment.

Results

KDM5 is essential for proper MB morphology

To assess the neurodevelopmental consequences resulting from KDM5 loss, we examined MB morphology of animals that were homozygous for a kdm5 null allele, kdm5140 (Drelon et al., 2018; Drelon et al., 2019). As homozygous kdm5140 animals fail to eclose from their pupal cases, we performed our immunohistochemical analyses on pharate adults, which externally appear indistinguishable from wild-type animals (Drelon et al., 2018). Following a well-established classification scheme used by others (Gombos et al., 2015; Kelly et al., 2016; Michel et al., 2004), MB defects were categorized as impacting MB growth and/or guidance, with the former defined by a stunted, overextended, or absent lobe and the latter by a misprojected lobe. Staining using an antibody specific to the NCAM-like cell adhesion molecule fasciclin 2 (Fas2) revealed highly penetrant MB abnormalities, with ~70% of animals exhibiting growth or guidance defects of the α/β lobes (Figure 1A, B). Interestingly, the predominant phenotype observed in our analysis was overextension of the β lobes and/or stunting of the α lobes. Because animals specifically lacking KDM5 demethylase activity have phenotypically normal α/β lobes (Zamurrad et al., 2018), our data demonstrate that KDM5 is required for correct MB morphology, independent of its canonical enzymatic function.

Figure 1. kdm5140 pharate adults have neuromorphological defects of the mushroom body (MB).

Figure 1.

(A) Representative α/β lobe Z projections of pharate wild-type (NP4707rev2 revertant) and kdm5140 strains. Arrows indicate growth defects, and arrowheads indicate guidance defects. The α/β lobes are revealed with anti-fasciclin 2. (B) Quantification of α/β lobe defects in wild-type and kdm5140 strains. n = 19–22 (mean n = 21). ***p<0.001 (chi-square test with Yates’ correction). (C) Western blot of w1118 and kdm5:HA adult heads confirming wild-type expression levels of lysine demethylase 5 (KDM5):HA within our endogenously tagged kdm5:HA strain. Anti-HA (top) and anti-KDM5 (bottom) loading control. (D) Schematic of an adult MB with its associated Kenyon cells (left). OK107-Gal4 is used to drive expression of UNC-84:GFP, an inner nuclear membrane GFP reporter, within MB neuroblasts, mushroom body-ganglion mother cells, and Kenyon cells (right). Brains are counterstained with anti-HA to demonstrate the presence of endogenously tagged KDM5:HA within Kenyon cell nuclei. Scale bars represent 20 μm. 

To assist in our functional understanding of KDM5, we sought to investigate its expression throughout central nervous system (CNS) development. To facilitate these analyses, we used CRISPR/Cas9 to generate a strain containing a 3xHA-tag fused to the endogenous locus of kdm5 that results in wild-type levels of protein expression (Figure 1C). We also confirmed that KDM5:HA was expressed in adult MB Kenyon cells by co-expression of a nuclear membrane-localized GFP reporter, UNC-84:GFP, with the MB driver OK107-Gal4 (Figure 1D). Immunostaining of the of wandering third-instar larvae revealed that KDM5 was localized to cortical nuclei while absent from neuropil-rich regions marked by the ubiquitous presynaptic active zone marker Bruchpilot (Brp) (Figure 2A). KDM5:HA localized to cortical nuclei across a variety of cell types, including neurons (Figure 2B), neuroblasts (NBs), and presumptive GMCs (Figure 2C). We also examined KDM5:HA expression in the adult brain, where KDM5:HA appeared to be similarly localized to cortical nuclei while absent from neuropil-rich regions, such as the antennal lobes and both the dorsolateral and ventrolateral protocerebra (Figure 2D, E). Given the broad expression pattern of KDM5:HA within a variety of cell types, KDM5 may regulate a range of neural processes, from NPC division and axonal growth to neuronal maturation and function.

Figure 2. lysine demethylase 5 (KDM5) is broadly expressed in nuclei of the Drosophila larval central nervous system (CNS) and adult brain.

Figure 2.

(A) Maximal Z projection of third-instar larval CNS revealing broad expression of endogenously tagged KDM5:HA and stained with anti-HA and anti-Bruchpilot (anti-Brp). (B) Cortical region of a larval (WL3) brain lobe stained with anti-HA and DAPI, showing nuclear localization of endogenously tagged KDM5:HA. (C) Neuroblast (NB)-specific wor-Gal4 driving expression of UNC-84:GFP to demonstrate endogenously tagged KDM5:HA expression within nuclei of WL3 central brain NBs (marked by *) and presumptive ganglion mother cells. UNC-84:GFP perdures for approximately 2–3 cell divisions. (D) Maximal Z projection of an adult brain revealing broad expression of endogenously tagged KDM5:HA with anti-HA and counterstained for neuropil with anti-Brp. (E) Dorsoanterior cortical region of an adult brain with endogenously tagged KDM5:HA. The nuclear localization of KDM5:HA is revealed by anti-HA, DAPI, and anti-Brp staining. Scale bars represent 50 μm in (A) and (D), 5 μM in (B) and (E), and 20 μM in (C).

KDM5 is required within neural precursors and immature neurons for proper MB morphology

To define the functional requirements of KDM5 during MB development, we utilized an inducible kdm5 shRNA transgene that we and others have shown effectively reduces KDM5 levels (Chen et al., 2019; Liu et al., 2014; Navarro-Costa et al., 2016). We first knocked down kdm5 broadly within all MBNBs, MB-GMCs, and Kenyon cells throughout development using OK107-Gal4 (Figure 3A, B). This resulted in significant neuromorphological defects of the α/β MB lobes, with the major phenotype being an overextension of the β lobes across the midline (Figure 3C, D). We next assessed the consequences of kdm5 depletion within distinct and overlapping subsets of mature Kenyon cells. Knocking down kdm5 using the mature Kenyon cell-specific Gal4 drivers C708a-, c305a-, H24-, and 201Y-Gal4 reduced KDM5 levels, but failed to produce significant gross morphological defects of the α/β lobes (Figure 3D, Figure 3—figure supplement 1). KDM5 is therefore unlikely to be required exclusively within mature, post-mitotic Kenyon cells for proper α/β lobe development.

Figure 3. Depletion of lysine demethylase 5 (KDM5) within neural precursors results in neuroanatomical defects of the mushroom body (MB).

(A) Schematic showing the sequential generation of distinct subclasses of Kenyon cells throughout development. MB neuroblasts (MBNBs) are eliminated via apoptosis (dotted line) immediately prior to eclosion. (B) OK107-Gal4 driving expression of UNC-84:GFP to reveal shRNA-mediated KDM5:HA depletion within adult Kenyon cell nuclei. (C) Representative Z projections of adult kdm5 knockdown animals exhibiting significant α/β lobe defects and their respective kdm5 shRNA and GAL4 controls. The antibody anti-fasciclin 2 is used to visualize α/β lobes. Arrows indicate growth defects, and arrowheads indicate guidance defects. (D) Quantification of α/β MB lobe defects in flies expressing kdm5 shRNA driven by neural progenitor cell- and Kenyon cell-specific drivers. ‘KD’ indicates shRNA-mediated knockdown of kdm5. n = 16–49 (mean n = 29). ****p<0.0001 (chi-square test with Bonferroni correction). (E) Z projection of larval cortex revealing wor-Gal4-driven expression of kdm5 shRNA and unc-84:gfp transgenes. KDM5:HA depletion is observed in presumptive ganglion mother cells and post-mitotic cells surrounding NBs (marked by asterisks). Scale bars represent 20 μm in (B) and (C) and 10 μm in (E).

Figure 3.

Figure 3—figure supplement 1. Validation of kdm5 knockdown within subpopulations of mature mushroom body (MB) neurons.

Figure 3—figure supplement 1.

MB Gal4 drivers promoting the expression of CD8:GFP to reveal shRNA-mediated lysine demethylase 5 (KDM5):HA depletion within subpopulations of mature Kenyon cells within the adult brain. Scale bar represents 5 μM.

In contrast, knockdown of kdm5 using two independent NB-restricted Gal4 driver lines, worniu-Gal4 (wor-Gal4) and inscuteable-Gal4 (insc-Gal4), resulted in profound α/β lobe defects (Figure 3C, D). Notably, a significant proportion of these brains simultaneously displayed both growth and guidance defects of the α/β lobes. Although wor-Gal4 expression is NB specific, KDM5 depletion and GFP expression continued to be observed in presumptive GMCs and post-mitotic cells surrounding each NB (Figure 3E). This is likely attributed to perdurance of the Gal4 activator protein and/or the kdm5 shRNA. KDM5 could therefore be required within the NB, GMC, or even post-mitotically within the immature neuron for proper α/β Kenyon cell neurodevelopment.

To increase the resolution of our analysis, we leveraged the restricted expression pattern of R71C09-Gal4, which drives expression within GMCs and early-born neurons of the Drosophila larval CNS (Figure 4ALi et al., 2014; Marshall and Brand, 2017; Aughey et al., 2018). R71C09-Gal4 did not appear to drive expression within NBs, including MBNBs (Figure 4A). When kdm5 was knocked down using R71C09-Gal4, KDM5 was dramatically depleted within presumptive GMCs and immature neurons (Figure 4B). We additionally noted that the axons of newly born Kenyon cells, which traverse through the core fibers of the MB pedunculus, were also labeled by an mVenus reporter when driven by R71C09-Gal4 (Figure 4C). Consistent with these observations, the labeled neurons were of α/β Kenyon cell origin, the last MB cell subtype to be born prior to eclosion (Figure 4C). Depletion of kdm5 using this driver resulted in profound α/β lobe defects, indicating that KDM5 is functionally required within GMCs and immature MB neurons for proper axonal development (Figure 4D). To assess sufficiency, we used R71C090-Gal4 to re-express kdm5 in GMCs and immature neurons of kdm5140 animals and found a significant recue of the defects we had previously observed (Figure 4E).

Figure 4. Expression pattern of R71C09-Gal4 within ganglion mother cells and immature neurons of the Drosophila central nervous system.

Figure 4.

(A) Whole-mount Z projections of a larval ventral nerve cord (left), brain lobe (middle), and brain cortical region (right). Z projections show R71C09-Gal4-driven expression of UNC-84:GFP, counterstained with neuroblast (NB)-specific anti-Dpn. (B) Z projection of larval cortex revealing R71C09-Gal4-driven expression of UNC-84:GFP with and without kdm5 shRNA. NBs are marked by an asterisk. (C) Optical sections of an adult mushroom body (MB) with its associated pedunculus, Kenyon cell bodies, and calyces expressing an R71C09-Gal4-driven mVenus reporter. R71C09-Gal4 strongly drives mVenus expression in newly born neurons located within core fibers of the pedunculus. (D) Representative Z projections (left) and quantification (right) of adult α/β MB lobe defects in flies expressing kdm5 shRNA driven by R71C09-Gal4. The antibody anti-fasciclin 2 (anti-Fas2) is used to visualize α/β lobes. n = 15–39 (mean n = 26). Arrowhead indicates a guidance defect. ****p<0.0001 (chi-square test with Bonferroni correction). (E) Representative α/β lobe Z projections and quantification of pharate wild-type (NP4707rev2 revertant), kdm5140, and kdm5140; R71C09 > kdm5 rescue strains. Arrows indicate growth defects. The α/β lobes are revealed with anti-Fas2. n = 20–28 (mean n = 24). **p<0.01; ***p<0.001 (chi-square test with Bonferroni correction). Scale bars represent 20 μm.

Transcriptional profiling using targeted DamID (TaDa) analyses reveals KDM5-regulatory networks critical for neurodevelopment

To understand how KDM5 functions within GMCs and immature neurons to regulate MB development, we used TaDa to carry out in vivo transcriptional profiling in a cell-type-specific and temporally controlled manner (Marshall et al., 2016a; Southall et al., 2013). By expressing Escherichia coli-derived DNA adenine methyltransferase (Dam) fused to Drosophila RNA polymerase II (Pol II) in MB-GMCs and immature Kenyon cells via the Gal4/UAS system, we surveyed genomic regions with altered Pol II occupancy upon depletion of KDM5. Dam-Pol II methylates adenine residues (m6A) at GATC motifs in close proximity to Pol II-occupied DNA, providing a surrogate for actively transcribed loci when normalized to expression of Dam alone (Doupé et al., 2018; Southall et al., 2013). As such, we refer to genomic loci with altered Pol II occupancy as differentially expressed genes (DEGs).

We expressed Dam-Pol II or Dam in GMCs and immature neurons of late third-instar larvae and pupae of homozygous kdm5140 animals using the R71C09-Gal4 driver (Figure 5A). Induction of the TaDa system during this developmental window predominantly resulted in the transcriptomic profiling of MB-GMCs and immature α/β Kenyon cells as these belong to one of the few cell lineages undergoing extensive neurogenesis during this time period and express R71C09-Gal4 (Technau and Heisenberg, 1982; Ito and Hotta, 1992; Truman and Bate, 1988). Importantly, the temperature shifts required to induce the TaDa system in a kdm5140 animals did not alter the frequency or type of structural MB defects observed (Figure 5—figure supplement 1).

Figure 5. Transcriptome profiling of lysine demethylase 5 (KDM5)-depleted ganglion mother cells (GMCs) and immature neurons by targeted DamID (TaDa) reveals KDM5-regulatory networks critical for ganglion mother cell (GMC) proliferation and neurodevelopment.

(A) Timeline of TaDa induction within GMCs and immature neurons of kdm5140 WL3 animals and pupae. (B) Volcano plot of differentially expressed genes (DEGs) within GMCs and immature neurons of kdm5140 animals compared to wild type. Genes with a false discovery rate (FDR) < 0.05 are in red, with those labeled involved in GMC proliferation and neurodevelopment. TaDa analyses were performed in quintuplicate. (C) Representative distribution of ontology terms for DEGs in kdm5140 GMCs and immature neurons using a PANTHER Overrepresentation Test (Fisher’s exact test with FDR < 0.05). (D) Timeline of TaDa and kdm5 shRNA induction within GMCs and immature neurons of WL3 animals and pharate adults. (E) Volcano plot of DEGs within kdm5 shRNA GMCs and immature neurons compared to those expressing a scrambled shRNA. Genes with an FDR < 0.05 are in red, with those labeled involved in GMC proliferation and neurodevelopment. TaDa analyses were performed in triplicate. (F) Correlation of Z scores between DEGs of kdm5140 and kdm5 shRNA TaDa datasets (Deming regression; p<0.0001). (G) Correlation of Z scores between overlapping DEGs of kdm5140 and kdm5 shRNA TaDa datasets (Deming regression; p<0.0001). (H) Representative distribution of ontology terms for DEGs in overlapping kdm5140 and kdm5 shRNA TaDa datasets utilizing a PANTHER Overrepresentation Test (Fisher’s exact test with FDR < 0.05).

Figure 5.

Figure 5—figure supplement 1. kdm5140 pharate adults expressing targeted DamID (TaDa) genetic elements present with significant mushroom body (MB) morphological defects.

Figure 5—figure supplement 1.

(A) Representative α/β lobe Z projections of pharate kdm5140 animals expressing TaDa genetic elements and their respective controls. The antibody anti-fasciclin 2 is used to visualize α/β lobes. Arrows indicate growth defects. Scale bar represents 20 μm. (B) Quantification of α/β lobe defects in pharate kdm5140 animals expressing TaDa genetic elements and their respective controls. n = 8–22 (mean n = 13). ****p<0.0001 (chi-square test with Bonferroni correction).
Figure 5—figure supplement 2. Knockdown of myc within Kenyon cells does not result in morphological defects of the mushroom body.

Figure 5—figure supplement 2.

Representative α/β lobe Z projections of adults expressing myc dsRNA driven by OK107-Gal4. No gross morphological defects were detected in the KD or control conditions n = 29–30.
Figure 5—figure supplement 3. Chromatin accessibility profiling using targeted DamID (CATaDa) analyses reveal minimal changes to chromatin accessibility within ganglion mother cells (GMCs) and immature neurons upon loss of kdm5.

Figure 5—figure supplement 3.

(A) Representative CATaDa profiles of R71C09-Gal4 expressing cells for kdm5140 and wild type. Heat maps show Dam binding profiles for the greatest 8181 peaks for each genotype. (B) Volcano plot showing changes to chromatin accessibility within GMCs and immature neurons of kdm5140 animals compared to wild type. Genes with an FDR < 0.01 are in yellow. CATaDa analyses were performed in quintuplicate. (C) Volcano plot showing changes to chromatin accessibility for differentially expressed genes (DEGs) from kdm5140 TaDa. In yellow are kdm5140 TaDa DEGs with an FDR < 0.05. In red are kdm5140 TaDa DEGs with associated changes in chromatin accessibility for FDR < 0.01 (Fisher’s exact test, p=4.79E-09).
Figure 5—figure supplement 4. Adults expressing R71C09-Gal4-driven kdm5 shRNA in tandem with targeted DamID (TaDa) genetic elements present with significant mushroom body morphological defects.

Figure 5—figure supplement 4.

(A) Representative α/β lobe Z projections of adult R71C09 > kdm5 shRNA animals expressing TaDa genetic elements and their respective controls. The antibody anti-fasciclin 2 is used to visualize α/β lobes. Arrowheads indicate guidance defects. Scale bar represents 20 μm. (B) Quantification of α/β lobe Z projections of adult R71C09 > kdm5 shRNA animals expressing TaDa genetic elements and their respective controls. n = 16–34 (mean n = 28). ****p<0.0001 (chi-square test with Bonferroni correction).
Figure 5—figure supplement 5. Transcriptome analyses reveal that lysine demethylase 5 (KDM5)-regulated gene expression is tissue-specific.

Figure 5—figure supplement 5.

(A) Venn diagram illustrating intersection of kdm5140 and kdm5 shRNA overlapping targeted DamID (TaDa) data with that from a previously published kdm5140 mRNA-seq wing disc dataset (Drelon et al., 2018). (B) Correlation of Z scores between kdm5140 TaDa and kdm5140 wing disc mRNA-seq (Drelon et al., 2018) datasets. Pearson’s R = 0.09.

Carrying out TaDa from five biological replicates, we identified a total of 636 DEGs using a statistical cutoff of FDR < 0.05, 438 of which were downregulated and 198 of which were upregulated (Figure 5B; Supplementary file 1). Consistent with previously published mRNA-seq data in Drosophila and other organisms, loss of KDM5 resulted in moderate changes to gene expression, with an average 2.4-fold decrease among downregulated genes and 2.1-fold increase among upregulated genes (Drelon et al., 2018; Iwase et al., 2016; Liu et al., 2014; Liu and Secombe, 2015; Lloret-Llinares et al., 2012; Lopez-Bigas et al., 2008; Lussi et al., 2016; Zamurrad et al., 2018). Thus, KDM5 likely functions within this cellular subpopulation to fine-tune gene expression across a number of pathways that are critical for MB development.

To determine if these dysregulated genes were enriched for functional categories, we used the program GeneOntology (The Gene Ontology Consortium, 2019; The Gene Ontology Consortium, 2000; The Gene Ontology Consortium, 2019), which utilizes the PANTHER Classification System (Mi et al., 2013) to mine gene ontology (GO) information. This revealed significant enrichment for categories involved in GMC fate determination (e.g., pros, brat, mira, cas), neural precursor cell proliferation (e.g., pros, wor, ase, esg, sna, E(spl)mγ-HLH, E(spl)mβ-HLH), axon guidance (e.g., pros, dac, brat, Hr51, chic, tap, pdm3, nerfin-1, lola), and cytoplasmic translation, among others (Figure 5C; Supplementary file 2). The finding that ribosomal protein genes, such as RpS2, RpS24, RpS28b, RpL3, Rpl23, RpL39, and RpL41, were altered in our kdm5140 TaDa data is consistent with our previously reported RNA-seq data from adult heads of demethylase-dead kdm5 strains showing that genes required for cytoplasmic translation were affected (Zamurrad et al., 2018). Interestingly, however, knockdown of the transcription factor myc, which regulates the expression of most ribosomal protein genes (Bellosta and Gallant, 2010; Grewal et al., 2005), in MBNBs, GMCs, and Kenyon cells using the OK107-Gal4 driver, did not result in any gross morphological defects of the α/β lobes (Figure 5—figure supplement 2). These data show that our observed MB phenotypes are not due to the regulation of cytoplasmic translation by KDM5.

H3K4me3 marks have been shown to be associated with regions of accessible chromatin, which often contain regulatory DNA sequences such as promoters and enhancers (Bhaumik et al., 2007; Li et al., 2012; Park et al., 2020; Thurman et al., 2012). If KDM5 functions independent of its H3K4me3 demethylase activity within GMCs and immature neurons to regulate MB development, loss of KDM5 may have only minimal effects on chromatin accessibility within this cellular subpopulation. To measure changes to chromatin landscape upon KDM5 loss, we utilized chromatin accessibility profiling using targeted DamID (CATaDa) (Aughey et al., 2018; Aughey et al., 2019). Dam methylates regions of highly accessible chromatin, thus providing an in vivo surrogate for chromatin accessibility (Aughey et al., 2018; Aughey et al., 2019). Comparing Dam methylation levels in R71C09-Gal4 expressing cells between kdm5140 and wild-type animals revealed minor changes to chromatin accessibility at a cutoff of FDR < 0.01 (Figure 5—figure supplement 3A, B). Overlapping reduced accessibility regions with the downregulated genes from our TaDa analysis revealed only 17 genes associated with reduced chromatin accessibility in GMCs and immature neurons at a cutoff of FDR < 0.01 (Figure 5—figure supplement 3C). Although this represented a significantly enriched proportion of the total number of genes associated with changes in chromatin accessibility (p=4.787E-09), they were not enriched via GO analysis for any biological categories. Because animals lacking demethylase activity do not present with gross MB morphological defects (Zamurrad et al., 2018), KDM5 functions to regulate MB morphology largely via demethylase-independent transcriptional mechanisms that do not dramatically alter chromatin accessibility.

Since kdm5140 animals have chronic loss of kdm5 in all tissues, the gene expression changes we observed may be the result of both cell-autonomous and non-cell-autonomous effects. To look directly at the KDM5-regulated transcriptome in GMCs and early-born neurons, we performed TaDa in animals expressing a kdm5 shRNA transgene under the control of R71C09-Gal4. In addition to utilizing a Dam-only control, we also accounted for activation of the RNAi pathway by expressing a scramble shRNA transgene under identical conditions. To ensure that changes to gene expression were reflective of the severe MB phenotypes we had previously observed, we induced the expression of kdm5 shRNA and dam-pol II for 6 days, beginning during the early second-instar larval (L2) stage, to allow for sufficient depletion of KDM5 within MB-GMCs and immature α/β Kenyon cells (Figure 5D). This temporally targeted knockdown strategy recapitulated the adult α/β lobe phenotypes we had observed for constitutive kdm5 knockdown with R71C09-Gal4 (Figure 5—figure supplement 4). From three biological replicates and a statistical cutoff of FDR < 0.05, we identified 1069 DEGs, 659 of which were downregulated and 410 of which were upregulated (Figure 5E; Supplementary file 3). Compared to the kdm5140 TaDa, the greater number of DEGs from the kdm5 RNAi TaDa could be attributed to the extended duration of the kdm5 knockdown and induction of Dam-Pol II. Additionally, we observed larger changes to gene expression, with an average 5.5-fold decrease among downregulated genes and a 4.3-fold increase among upregulated genes (Figure 5E; Supplementary file 3). This could be ascribed to the acute loss of kdm5 expression caused by RNAi-mediated depletion, which would decrease the likelihood of compensatory changes occurring.

To obtain a list of high-confidence genes regulated by KDM5, we compared the DEGs found in our kdm5140 and kdm5 knockdown TaDa datasets. This revealed a total of 335 overlapping dysregulated genes, 319 of which were up- or downregulated in both datasets (r = 0.68, p<0.0001) (Figure 5F, G; Supplementary file 4). GO analysis of the 319 similarly dysregulated genes revealed an even greater enrichment in categories related to GMC fate determination (pros, mira, cas), neural precursor cell proliferation (wor, esg, pros, mira, sna), and axon guidance (pros, chic, lola, Oli, tap, Nrt, nerfin-1, Hsc70-4), among others (Figure 5H; Supplementary file 4). Interestingly, only 17 of these genes overlapped with our previously published kdm5140 wing disc RNA-seq data (Drelon et al., 2018; Figure 5—figure supplement 5). This nonsignificant overlap suggests that KDM5 functions in a predominantly tissue- and cell-specific manner to regulate the expression of downstream targets.

To identify biologically relevant functional networks for the 319 KDM5-regulated, high-confidence genes, we performed gene network and community clustering analyses (Morris et al., 2011; Shannon et al., 2003). These analyses revealed seven discrete networks with greater than two nodes (Figure 6A). Of these, two networks were highly enriched for genes implicated in MB development (toy and tap), neural precursor cell proliferation (pros, ase, wor, mira, sna, esg, E(spl)mγ-HLH, and E(spl)mβ-HLH), and axon growth and guidance (pros, nerfin-1, tap, emc, Nrt, and elav).

Figure 6. Network of known and predicted interactions between lysine demethylase 5 (KDM5)-regulated aenes and analysis of direct KDM5 targets.

Figure 6.

(A) Gene network analysis and community clustering were performed using Cytoscape with a minimum confidence score of 0.4. Networks with greater than two nodes are shown and color-coded based on cluster. Labels indicate general categories of overlapping kdm5140 and kdm5 shRNA targeted DamID (TaDa) differentially expressed genes (DEGs) within each cluster. (B) Venn diagram illustrating intersection of similarly dysregulated kdm5140 and kdm5 shRNA overlapping DEGs with direct KDM5 targets from R71C09 > dam-kdm5 TaDa. (C) Analysis of the 319 similarly dysregulated kdm5140 and kdm5 shRNA overlapping DEGs (with values plotted from the kdm5 shRNA TaDa). Direct KDM5 targets are labeled in orange. Venn diagram created with BioVenn (Hulsen et al., 2008).

To determine which genes affected by loss of KDM5 were likely to be direct targets, we generated a transgenic Drosophila strain expressing Dam fused to KDM5 under the control of UAS (UAS-dam-kdm5). To match the developmental window utilized for our RNA pol II TaDa analyses, we expressed Dam-KDM5 or Dam in late third-instar larvae and pupae using the R71C09-Gal4 driver. From six biological replicates, we identified a total of 4399 genes with KDM5 peaks using a statistical cutoff of FDR < 0.01 (Supplementary file 5). Of these, 120 genes overlapped with the 319 genes we previously identified as being KDM5-regulated and represented a significant enrichment (Figure 6B, C; p=6.396E-07). Of particular interest was one gene, prospero (pros), which encodes a homeodomain-domain-containing transcription factor that is a critical regulator of axon pathfinding and growth (Doe et al., 1991; Froldi et al., 2015; Tea et al., 2010; Vaessin et al., 1991). Our Dam-KDM5 occupancy data showed that KDM5 binds to TSS within the pros locus (Figure 7A) and, together with our Pol II TaDa data, suggest that KDM5 directly regulates pros expression.

Figure 7. Neuromorphological and transcriptomic analyses reveal a genetic interaction between prospero and lysine demethylase 5 (KDM5).

Figure 7.

(A) Integrative genomics viewer (IGV) plot showing average KDM5 occupancy at pros transcriptional start sites across six replicates. Scale bars represent the log2 ratio change between Dam-KDM5 and Dam samples. Called peaks are indicated by gray bars. (B) Venn diagram illustrating intersection of similarly dysregulated kdm5140 and kdm5 shRNA overlapping differentially expressed genes (DEGs) with Dam-KDM5 direct binding data and a previously published Dam-Pros targeted DamID (TaDa) binding dataset (Fisher’s exact test, p=2.20E-16 for KDM5-dysregulated and Pros gene overlap) (Liu et al., 2020). (C) Analysis of the 319 similarly dysregulated kdm5140 and kdm5 shRNA overlapping DEGs (with values plotted from the kdm5 shRNA TaDa). Direct Pros targets from the previously published Dam-Pros TaDa dataset (Liu et al., 2020) are labeled in orange. (D) Representative Z projections of OK107 > pros RNAi adults exhibiting significant α/β lobe defects. The α/β lobes are revealed by anti-fasciclin 2 (Fas2). (E) Quantification of α/β mushroom body (MB) lobe defects in flies expressing pros shRNA driven by OK107-Gal4. n = 16–19 (mean n = 17). ****p<0.0001 (chi-square test with Bonferroni correction). (F) Representative Z projections of representative kdm5140/+ and pros17/+ heterozygous adult α/β MB lobes (top) and kdm5140/+; pros17/+ transheterozygous adult α/β MB lobes (bottom). The α/β lobes are revealed by anti-Fas2. (G) Quantification of α/β MB lobe defects in kdm5140/+; pros17/+ transheterozygous adults and heterozygous controls. n = 28–37 (mean n = 34). ****p<0.0001 (chi-square test with Bonferroni correction). (H) Model proposing a genetic interaction between pros and kdm5 within ganglion mother cells (GMCs) and immature α/β Kenyon cells. KDM5 binds to the pros locus and positively regulates its transcription. Loss of kdm5 leads to downregulation of pros and its targets, resulting in defects to MB neurodevelopment and cognitive function. Image was created with BioRender and Venn diagrams with BioVenn (Hulsen et al., 2008). Scale bars represent 20 μm.

The transcription factor pros genetically interacts with kdm5 and is required for proper MB development

If KDM5 functions upstream of Pros to regulate its expression, it is likely that KDM5 may also indirectly regulate the expression of Pros targets. To explore this possibility, we compared our analyses with published Dam-Pros occupancy data within GMCs and immature neurons (Liu et al., 2020; Gene Expression Omnibus [GEO] accession number GSE136413). Strikingly, 45% (142 of 319) of KDM5-regulated genes were bound by Pros (p=2.20E-16, Fisher’s exact test) (Figure 7B, C; Supplementary file 6). In addition, a majority of Pros-bound targets were not co-bound by KDM5 (Figure 7B). These data suggest that Pros could be a key mediator of KDM5 function in GMCs and immature neurons. Our data also implicate Pros in the activation of genes as the majority of Pros-bound genes were downregulated upon KDM5 depletion (Figure 7C).

While roles for Pros in regulating NB and GMC asymmetric division are well-described (Choksi et al., 2006; Doe et al., 1991; Vaessin et al., 1991), its role in MB development remains unexplored. R71C09-Gal4-mediated knockdown of pros resulted in early larval lethality, preventing us from examining MB phenotypes. However, depleting Pros within the smaller cellular population of MB progenitor and Kenyon cells using OK107-Gal4 allowed for the formation of viable adults. Analyses of these adult brains revealed a complete disruption of MB morphology, with both growth and guidance defects present in all animals observed (Figure 7D, E).

As kdm5 or pros depletion within MB progenitor and Kenyon cells each independently resulted in significant axonal defects, we next assessed whether kdm5 and pros functioned within the same genetic pathway. To test this, we generated animals heterozygous for null alleles of both kdm5 and pros, thereby simultaneously reducing levels of both proteins. Animals heterozygous for either kdm5140 or pros17 did not display significant MB defects. In contrast, adults that were transheterozygous for kdm5140 and pros17 displayed MB abnormalities (Figure 7F, G). These data are consistent with KDM5 and Pros functioning synergistically to affect MB development. KDM5 is therefore likely to function within GMCs and immature neurons to modulate levels of pros and its downstream targets, thereby regulating MB development (Figure 7H).

Discussion

Here, we utilize in vivo transcriptional and binding approaches coupled with neuromorphological analyses to demonstrate that KDM5 is essential for the development of the adult MB. Although previous work in murine models has shown that knockout of the kdm5 ortholog Kdm5c leads to dendritic spine abnormalities in cortical brain regions, these studies utilized ubiquitous knockout systems that were not amenable to spatiotemporal genetic manipulation (Iwase et al., 2016; Vallianatos et al., 2020). Drosophila, however, provides a genetically tractable system to modulate gene expression in a spatiotemporally restrictive manner. Using the MB as a model for neuronal development, we demonstrate that KDM5 expression within GMCs and immature neurons is necessary and sufficient for proper neuropil development. Depletion of KDM5 within mature subpopulations of Kenyon cells did not affect gross MB structure, suggesting that there exists a critical neurodevelopmental window during which KDM5 is required to regulate genetic programs essential for axonal growth and guidance. Notably, the MB growth and guidance defects we observed upon depletion of KDM5 phenocopied those seen in other Drosophila ID models, such as the fmr1 and dnab2 RNA binding protein mutants (Kelly et al., 2016; Michel et al., 2004). It is thus possible that ID genes may function synergistically within common neurodevelopmental pathways to affect neuronal architecture and function.

We additionally performed RNA pol II TaDa analyses within GMCs and immature neurons to survey gene expression changes resulting from loss of kdm5. Although previous transcriptomic studies have utilized extracted RNA from dissected tissues or from neuronal or fibroblast cultures as input for RNA-seq (Brookes et al., 2015; Iwase et al., 2016; Liu and Secombe, 2015; Scandaglia et al., 2017; Vallianatos et al., 2020; Wei et al., 2016; Zamurrad et al., 2018), this is the first study describing changes to gene expression resulting from cell-specific loss of kdm5. This technique allowed us to survey gene expression changes within GMCs and immature neurons of kdm5 null and cell-type-specific knockdown animals, revealing a number of DEGs associated with neurodevelopment. It is important to note, however, that since we induced expression of UAS-dam and UAS-dam-Pol II beginning during the late larval stages, a subset of our data may reflect changes to gene expression within GMCs and immature neurons that are not part of the MB lineage. Nevertheless, TaDa induction during the larval stage was brief (<24 hr at 29°C), with the vast majority of transcriptomic changes occurring within MB-GMCs and immature Kenyon cells, which are some of the few cell types able to proliferate up to 96 hr after pupal formation (Truman and Bate, 1988).

Our data suggest that one means by which KDM5 functions in neurons is by binding to and regulating the expression of the homeodomain-containing transcription factor Pros. Pros is a cell fate determinant that is expressed in most neuronal precursors and immature neurons and is involved in cell cycle exit, neuronal differentiation, and axonal development (Choksi et al., 2006; Doe et al., 1991; Froldi et al., 2015; Tea et al., 2010; Vaessin et al., 1991). Loss of pros results in axonal routing defects within embryonic motor and sensory neurons (Vaessin et al., 1991) and can lead to the miswiring of olfactory projection neuron dendrites (Miyoshi et al., 2015; Tea et al., 2010). However, despite its demonstrated importance in other neuronal contexts, a role for Drosophila Pros or its orthologs in regulating axonal growth and guidance programs has remained largely uncharacterized.

Pros is evolutionarily well-conserved, with the mammalian ortholog PROX1 having been shown to promote neuronal differentiation and migration. Specifically, studies utilizing chick and mouse neural progenitor cells demonstrate that PROX1 is essential for cell cycle exit and differentiation of neuronal precursors via the transcriptional repression of Notch1 (Kaltezioti et al., 2010; Lavado et al., 2010). Additionally, studies leveraging conditional knockout mouse strains have shown that PROX1 regulates GABAergic cortical interneuron migration during embryonic and postnatal development (Miyoshi et al., 2015). However, despite its demonstrated importance in other neuronal contexts, a role for PROX1 or its orthologs in regulating axonal growth and guidance programs has remained largely uncharacterized. Here, we demonstrate that depletion of Drosophila Pros within MBNBs, MB-GMCs, and immature Kenyon cells leads to severe MB growth and guidance defects. Importantly, Pros is expressed primarily in neural precursors and immature neurons at a time when KDM5 function is critical for MB development (Doe et al., 1991; Vaessin et al., 1991).

Our transcriptomic data further reveal that a number of Pros interactors are dysregulated upon kdm5 depletion. For example, the direct Pros target nerfin-1, which encodes a zinc finger transcription factor required for early axon pathfinding by most CNS neurons in the Drosophila embryo (Kuzin et al., 2005; Liu et al., 2020), was significantly downregulated. Additionally, genes encoding Toy and Tap, which have been shown to regulate MB morphology (Furukubo-Tokunaga et al., 2009; Yuan et al., 2016), were similarly affected. Transcriptional dysregulation of pros and its targets may thus provide important molecular insight into the neuronal phenotypes associated with kdm5 loss of function.

Although we demonstrate that KDM5 binds to the pros gene and is needed for its activation, we do not know the precise mechanism by which this occurs. We predict, however, that it is independent of its canonical histone demethylase activity as animals lacking this enzymatic function have MBs that are phenotypically indistinguishable from those of wild type (Zamurrad et al., 2018). Because flies lacking KDM5 histone demethylase activity have cognitive deficits (Zamurrad et al., 2018), it is likely that KDM5 regulates neuronal development and function via multiple distinct mechanisms. Consistent with this model, several ID-associated mutations in KDM5C have been shown to alter H3K4me3-directed enzymatic activity in vitro, whereas others do not (Brookes et al., 2015; Vallianatos et al., 2018). Based on these data and the results reported here, it is likely that ID-causing mutations in human KDM5A, KDM5B, or KDM5C might fall into three classes: the first may only affect KDM5 demethylase activity, the second may only affect non-enzymatic activities necessary for transcriptional regulation, and the third may affect both. The extent to which canonical and non-canonical activities are disrupted may, indeed, correlate with the severity of the cognitive deficit or presence of syndromic features. Future work will further elucidate how individual patient mutations may disrupt these transcriptional regulatory mechanisms to influence neuronal and behavioral outputs, thus providing a promising strategy for predicting and treating patients with pathogenic variants in KDM5 family genes.

Materials and methods

Resource availability

Lead contact

Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Julie Secombe (Julie.Secombe@einsteinmed.org).

Materials availability

The kdm5:HA and UAS-LT3-dam-kdm5 strains generated in this study are available from the Lead Contact without restriction. DamID-Seq data have been deposited in the GEO under accession number GSE156010 for Dam-Pol II TaDa and GSE166116 for Dam-KDM5 TaDa.

Code availability

The code supporting the current study is available at https://github.com/owenjm/polii.gene.call (Marshall et al., 2013), https://github.com/owenjm/damidseq_pipeline (Marshall and Brand, 2015b), and https://github.com/owenjm/find_peaks (Marshall et al., 2016b).

Fly strains and genetics

A detailed list of the genotypes of the flies used in each figure is included in the Key Resources Table in the Appendix.

All GAL4 and GMR GAL4 lines were generated at the Janelia Research Campus/HHMI (Pfeiffer et al., 2008Jenett et al., 2012) and were obtained from the Bloomington Drosophila Stock Center (BDSC) at Indiana University.

The following transgenes were used: UAS-kdm5-shRNA (RRID:BDSC_35706), 20XUAS-IVS-CsChrimson.mVenus (RRID:BDSC_55136), UAS-tub-GAL80ts (RRID:BDSC_7108), UAS-pros-dsRNA (RRID:BDSC_42538), and UAS-myc-dsRNA (VDRC stock# KK106066). The UAS-LT3-dam and UAS-LT3-dam-pol II lines were kindly shared by Andrea Brand (U. Cambridge, Gurdon). The 5XUAS-unc84-2Xgfp line was kindly shared by Gilbert Henry and Todd Laverty (HHMI Janelia). The kdm5140 mutant allele and UAS-kdm5 transgene have been previously described (Drelon et al., 2018; Secombe et al., 2007).

Cloning and transgenesis

To tag the endogenous kdm5 locus with three in-frame HA epitope tags, we used CRISPR/Cas9-mediated knock-in. The HA epitopes and the homology arms, carrying a synonymous mutation for the PAM sequence, were PCR amplified from a clone containing the kdm5 locus from the wild-type strain w1118 (pattB.gkdm5; Navarro-Costa et al., 2016). The donor DNA repair template consisted of three PCR fragments cloned, by In-Fusion HD (Takara, Bio), into the pHD-ScarlessDsRed vector (Addgene plasmid #51434). AarI and SapI enzymes were used to linearize the plasmid. The flyCRISPR Optimal Target Finder tool was used to select the target genomic cleavage and design the gRNA (http://targetfinder.flycrispr.neuro.brown.edu). The top and bottom oligos for the gRNA were phosphorylated and annealed using T4 polynucleotide kinase (NEB) and then cloned into the pU6 vector (Addgene plasmid #53062), which was linearized with BpiI (NEB). The gRNA and donor DNA were sent to the BestGene for injection into embryos expressing Cas9 in the germline (y1, M{vas-Cas9.RFP-}ZH-2A, w1118; RRID:BDSC_55821). To remove the DSRed cassette, transformed flies were balanced and then crossed with flies carrying piggyBac transposase (RRID:BDSC_32073). Flies lacking expression of RFP were recovered and homozygous stocks were sequenced for the kdm5 locus. The correct removal of the DSRed cassette and presence of the 3xHA tag were confirmed by PCR sequencing and western blot.

To generate UAS-LT3-dam-kdm5 flies, kdm5 cDNA was PCR amplified from pB-Lid and cloned into the pUAST-attB-LT3-NDam vector (Marshall et al., 2016a). pUAST-attB-LT3-NDam was linearized with Notl and Xbal, and the cut plasmid and PCR insert were assembled by In-Fusion HD (Takara, Bio). The construct was sequenced and sent to BestGene for injection into embryos carrying the attP2 landing site (RRID:BDSC_8622).

Immunohistochemistry

For fixation of late third-instar larval brain tissue, the CNS was first removed via dissection in cold phosphate buffered saline (PBS, pH 7.4) and allowed to incubate in fixation buffer (4% paraformaldehyde in PBS) at room temperature (RT) for 40 min. After washing for three cycles of 15 min in PBS with 0.2% Triton X-100 (0.2% PBT), brains were incubated with blocking buffer (5% normal goat serum in 0.2% PBT) for 30 min at RT. The brains were then incubated with primary antibodies for 2 days at 4°C and washed for three cycles of 15 min in 0.2% PBT. Secondary antibodies were then added and brains were incubated for 2 days at 4°C. After being washed in PBS for three cycles of 15 min at RT, brains were incubated in a drop of Vectashield mounting medium (Vector Laboratories, H-1000) or DAPI Fluoromount G (SouthernBiotech, OB010020) overnight at 4°C. Brains were then mounted on glass slides (Superfrost Plus, Fisherbrand), flanked by glass spacers and covered with a final glass coverslip before being used for image analysis.

A similar protocol was followed for immunostaining of pharate adult and 3- to 5-day-old adult brain tissue with the following exceptions. Pharate adult heads or whole adult animals were allowed to incubate in fixation buffer (4% paraformaldehyde in 0.2% PBT) at 4°C for 3 hr. Heads or whole animals were then washed in 0.2% PBT for three cycles of 15 min at RT. Brains were then dissected from the fixed tissue and blocked for 30 min at RT. Antibody incubation and mounting was identical to that described above.

The following primary antibodies were used: mouse anti-brp (1:50, DSHB cat# nc82, RRID:AB_2314866), mouse anti-Fas2 (1:25, DSHB cat# 1D4 anti-fasciclin II, RRID:AB_528235), rabbit anti-HA (1:100, Cell Signaling Technology cat# 3724; RRID:AB_1549585), mouse anti-HA (1:100, Cell Signaling Technology cat# 2367, RRID:AB_10691311), and rat anti-Dpn (1:100, Abcam cat# ab195173; RRID:AB_2687586). Primary antibodies were prepared in 5% NDS/0.2% PBT with 0.02% NaN3. The following secondary antibodies were used: goat anti-mouse Alexa-488 (1:500, Thermo Fisher Scientific cat# A32723; RRID:AB_2633275), goat anti-mouse Alexa-568 (1:500, Thermo Fisher Scientific cat# A11004; RRID:AB_2534072), goat anti-rabbit Alexa-488 (1:500, Thermo Fisher Scientific cat# A11034; RRID:AB_2576217), goat anti-rabbit Alexa-568 (1:500, Thermo Fisher Scientific cat# A11004, RRID:AB_2534072), and goat anti-rat Alexa 568 (1:500, Thermo Fisher Scientific cat# A11077; RRID:AB_2534121). All secondary antibodies were diluted in 5% NDS/0.2% PBT.

Western blotting

Western analysis was carried out as previously described (Drelon et al., 2019). Briefly, 3- to 5-day-old adult fly heads were homogenized in 2× NuPAGE LDS sample buffer, sonicated for 10 min, treated with DTT, run on a 4–12% Bis-Tris 1 mm gel, and transferred to a PVDF membrane. The following primary antibodies were used: rabbit anti-KDM5 (1:1000, Secombe J; Genes Dev. 2007; cat# lid, RRID:AB_2569502) and mouse anti-HA (1:1000, Cell Signaling Technology cat# 2367, RRID:AB_10691311). The following secondary antibodies were used: IRDye 680RD donkey anti-mouse IgG (1:8000; LI-COR Biosciences cat# 925-68072, RRID:AB_2814912) and IRDye 800CW donkey anti-rabbit IgG (1:8000; LI-COR Biosciences cat# 926-32213, RRID:AB_621848). Blots were scanned and processed using a LI-COR Odyssey Infrared scanner.

Image acquisition

All tissue images were taken on a Leica SP8 confocal microscope using either a ×20 air lens (N.A. = 0.75 air, W.D. = 0.64 mm) or a ×63 immersion lens (N.A. = 1.4 oil, W.D. = 0.14 mm). All MB confocal stacks were taken under either ×2 or ×2.5 zoom, in a 1024 × 1024 configuration and using 1 μm resolution. Image stacks were processed with Figi (ImageJ). Figures were composed using Microsoft Powerpoint.

Targeted DamID and analyses

To profile Pol II occupancy in GMCs and immature neurons of kdm5140 pupae, kdm5140/CyO:gfp; UAS-LT3-dam or kdm5140/CyO:gfp; UAS-LT3-dam-pol II flies were crossed with kdm5140/CyO:gfp; R71C09-Gal4 flies and were allowed to lay eggs overnight for 12–14 hr at 25°C. Embryos were then moved to 18°C for a period of 16 days, and GFP-negative wandering third-instar larvae were transferred to a restrictive temperature of 29°C for 3 days to induce the expression of the UAS-dam and UAS-dam-pol II transgenes. As a parallel control, kdm5 wild-type animals carrying UAS-LT3-dam or UAS-LT3-dam-pol II were crossed with R71C09-Gal4 and were also allowed to lay eggs for 24 hr at 25°C. Embryos were then moved to 18°C for 9 days and subsequently transferred to 29°C for 3 days.

For kdm5 knockdown experiments, flies carrying either UAS-LT3-dam; UAS-kdm5_shRNA or UAS-LT3-dam-pol II; UAS-kdm5_shRNA were crossed with flies carrying UAS-tubulin-Gal80ts; R71C09-Gal4. Parallel crosses were also performed with flies bearing a scrambled shRNA sequence in place of kdm5 shRNA. All flies were allowed to lay eggs overnight for 12–14 hr at 25°C. Embryos were then transferred to 18°C for 4 days and then subsequently transferred to 29°C for 6 days.

To determine genomic regions directly bound by KDM5 within GMC and immature neurons, UAS-LT3-Dam or UAS-LT3-Dam-kdm5 animals were crossed with flies carrying tubulin-Gal80ts; R71C09-Gal4 and allowed to lay eggs for 12–14 hr at 25°C. Embryos were transferred to 18°C for 9 days and subsequently moved to 29°C for 3 days.

Tissue processing for all TaDa experiments was performed as previously described in Marshall et al., 2016a with the following modifications. A total of 40 pupae of each genotype were homogenized in 500 mM EDTA followed by DNA extraction using the Zymo Quick-DNA Miniprep Plus Kit. DpnI digestion, PCR adaptor ligation, DpnII digestion, and PCR amplification were performed as described. DNA was sonicated using a Diagenode Bioruptor for 8–10 cycles (5 min at high power, 30 s on/30 s off) and analyzed using an Agilent Bioanalyzer. DamID adaptor removal and DNA cleanup were performed as previously described (Marshall et al., 2016a), and samples were submitted to BGI for sequencing.

Sequencing libraries were prepared at BGI Genomics following a ChIP-seq workflow. DNA fragments were first end-repaired and dA-tailed using End Repair and A-Tailing enzyme. Adaptors were then ligated for sequencing and ligated DNA purified using AMPure beads. DNA was then PCR amplified with BGI primers for eight cycles and PCR purified with AMPure beads. DNA was then homogenized, circularized, digested, and again purified. DNA was then prepared into proprietary DNA nanoballs (DNB) for sequencing on a BGISEQ-500 platform with 50 bp read length and 20 M clean reads.

Quantification and statistical analyses

Statistical analyses

For MB morphological analyses, results are presented as bar plots for which percentage of brains with MB lobe growth and/or guidance defects are calculated. For these analysis, N = number of brains examined. ‘Growth defects’ were defined by an overgrown, stunted, or absent lobe, and ‘guidance defects’ were defined by a full or partially misprojected lobe. In the case where both defect types were observed in a single brain, the defect was categorized as a ‘growth and guidance defect’. All MB statistical analysis was performed using GraphPad Prism 8.4 (GraphPad Software, Inc, CA, USA). A 2 × N contingency table was used when comparing MB defects of more than two genotypes, where N = number of genotypes, and significance was determined using a chi-square test with either a Yates’ or Bonferroni correction with * p<0.05, ** p<0.01, *** p<0.001, and **** p<0.0001.

GO enrichment analysis was done using PANTHER Overrepresentation testing (http://geneontology.org; Mi et al., 2013) with a Fisher’s exact test (FDR < 0.05). Annotation version and release date: GO Ontology database DOI: 10.5281/zenodo.3873405, released 2020-06-01.

For targeted DamID analyses, sequencing data were aligned to release six of the Drosophila melanogaster genome and processed using damidseq_pipeline as previously described (Marshall and Brand, 2017; Marshall et al., 2016aMarshall and Brand, 2015b). RNA Pol II occupancy over gene bodies was calculated via polii.gene.call (Marshall et al., 2016aMarshall et al., 2013). DEGs were called via the NOIseq R package (Tarazona et al., 2011); briefly, RNA pol II gene occupancy scores were scaled, and inverse log values used as input to NOIseq with parameters of upper quantile normalization and biological replicates. DEGs were called with a q value of 0.95.

For CATaDa analyses, Dam-only BAM files generated via damidseq_pipeline were converted to 75 nt bins via bam2coverage, and replicates averaged. Peaks were called separately on the wild-type and mutant conditions via find_peaks (Marshall et al., 2016aMarshall et al., 2016b) with a minimum quantile of 0.95, before combining and merging peaks with BEDTools (Quinlan and Hall, 2010), and plotting heat maps with SeqPlots (Stempor and Ahringer, 2016). Average Dam occupancy values over peaks for each biological replicate were determined via polii.gene.call and differential occupancy called via NOIseq as with RNA Pol II occupancy above. Peaks called as significant were associated with the nearest gene promoter via peaks2genes (Marshall et al., 2016a).

For Dam-KDM5 analyses, after converting to bedgraphs via damidseq_pipeline as above, peaks were called using find_peaks (using the parameters fdr = 0.01, min_quant = 0.9) (Marshall et al., 2016a) on the averaged replicates, and genes overlapping peaks identified using peaks2genes (Marshall et al., 2016a).

Acknowledgements

We thank all the frontline and essential workers who worked tirelessly to protect and assist others during the COVID-19 pandemic. We additionally thank Nicholas Baker, Hannes Bülow, Andreas Jenny, Bernice Morrow, Anna Francesconi, and all members of the Secombe Lab for their feedback and edits on the manuscript. We appreciate the confocal microscope training and assistance provided to us by Hillary Guzik and members of the Einstein Analytical Imaging Facility (AIF). We thank Gilbert Henry and Andrea Brand for their generous donations of fly stocks and reagents. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were also used in this study. The 1D4 and 9.4A monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at the University of Iowa. We are additionally grateful to the NIH Special Instrument Grant S10OD023591 and the Cancer Center Support Grant P30CA013330. This research was supported by the NIH Ruth L Kirschstein National Research Service Award F31NS110278, the Einstein MSTP Training Grant T32GM007288, and the Junior Investigator in Neuroscience Research Award (JINRA) from the Dominick P Purpura Department of Neuroscience to HAMH, NIH R01GM112783 and support from the Irma T Hirschl Trust to JS, and NHMRC grants APP1128784 and APP1185220 to OJM.

Appendix 1

Appendix 1—key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Antibody Mouse monoclonal anti-brp DSHB Cat# nc82; RRID:AB_2314866 IF (1:50)
Antibody Rabbit monoclonal anti-HA-Tag Cell Signaling Technology Cat# 3724; RRID:AB_1549585 IF (1:100)
Antibody Mouse monoclonal anti-HA-Tag Cell Signaling Technology Cat# 2367; RRID:AB_10691311 IF (1:100)
WB (1:1000)
Antibody Rat monoclonal anti-Deadpan Abcam Cat# ab195173; RRID:AB_2687586 IF (1:100)
Antibody Goat polyclonal anti-mouse Alexa-488 Thermo Fisher Scientific Cat# A32723; RRID:AB_2633275 IF (1:500)
Antibody Goat polyclonal anti-mouse Alexa-568 Thermo Fisher Scientific Cat# A11004; RRID:AB_2534072 IF (1:500)
Antibody Goat polyclonal anti-rabbit Alexa-488 Thermo Fisher Scientific Cat# A11034; RRID:AB_2576217 IF (1:500)
Antibody Goat polyclonal anti-rabbit Alexa-568 Thermo Fisher Scientific Cat# A11004, RRID:AB_2534072 IF (1:500)
Antibody Goat polyclonal anti-rat Alexa-568 Thermo Fisher Scientific Cat# A11077; RRID:AB_2534121 IF (1:500)
Antibody Mouse monoclonal anti-Fas2 DSHB Cat# 1D4; RRID:AB_528235 IF (1:25)
Antibody Rabbit polyclonal anti-KDM5 Secombe Lab; Secombe et al., 2007 RRID:AB_2569502 WB (1:1000)
Antibody IRDye 680RD donkey monoclonal anti-mouse IgG secondary antibody LI-COR Biosciences LI-COR Biosciences Cat# 925-68072, RRID:AB_2814912 WB (1:8000)
Antibody IRDye 800CW donkey monoclonal anti-rabbit IgG secondary antibody LI-COR Biosciences LI-COR Biosciences Cat# 926-32213, RRID:AB_621848 WB (1:8000)
Cell line (Escherichia coli) NEB 5-alpha competent E. coli New England BioLabs Cat# C2987
Commercial assay or kit Clontech CloneAmp HiFi PCR Premix Clontech Cat# 639298
Commercial assay or kit Advantage 2 cDNA polymerase Clontech Cat# 639201
Commercial assay or kit Agencourt AMPure XP Beads Beckman Coulter Cat# A63880
Commercial assay or kit Takara In-Fusion HD Cloning Plus Takara Cat# 638909
Commercial assay or kit Quick​ ​Ligation ​Kit​ New England BioLabs Cat# M2200S
Commercial assay or kit Zymo​ ​Quick-DNA​ ​miniprep​ ​plus Zymo Research Cat#​ ​D4069
Commercial assay or kit Macherey-Nagel​ ​NucleoSpin​ ​Gel​ ​and​ ​PCR​ ​Clean-up​ Kit Takara Cat# 740609.250
Commercial assay or kit Qubit dsDNA HS Assay Kit Invitrogen Cat# Q32851
Genetic reagent (Drosophila melanogaster) Drosophila: kdm5:3xHA This study N/A Endogenous kdm5:HA strain (Figures 24). Available from lead contact.
Genetic reagent (D. melanogaster) Drosophila: OK107-Gal4 Bloomington Drosophila Stock Center RRID:BDSC_854
Genetic reagent (D. melanogaster) Drosophila: 5XUAS-unc84::2XGFP Janelia Research Campus; Henry et al., 2012 N/A
Genetic reagent (D. melanogaster) Drosophila: wor-Gal4 Bloomington Drosophila Stock Center RRID:BDSC_56553
Genetic reagent (D. melanogaster) Drosophila: UAS-kdm5RNAI Bloomington Drosophila Stock Center RRID:BDSC_35706
Genetic reagent (D. melanogaster) Drosophila: insc-Gal4 Bloomington Drosophila Stock Center RRID:BDSC_8751
Genetic reagent (D. melanogaster) Drosophila: c708a-Gal4 Bloomington Drosophila Stock Center RRID:BDSC_50743
Genetic reagent (D. melanogaster) Drosophila: c305a-Gal4 Bloomington Drosophila Stock Center RRID:BDSC_30829
Genetic reagent (D. melanogaster) Drosophila: H24-Gal4 Bloomington Drosophila Stock Center RRID:BDSC_51632
Genetic reagent (D. melanogaster) Drosophila: 201Y-Gal4 Bloomington Drosophila Stock Center RRID:BDSC_4440
Genetic reagent (D. melanogaster) Drosophila: UAS-Dcr-2 Bloomington Drosophila Stock Center RRID:BDSC_24650
Genetic reagent (D. melanogaster) Drosophila: GMR71C09-GAL4 Bloomington Drosophila Stock Center RRID:BDSC_39575
Genetic reagent (D. melanogaster) Drosophila: 20XUAS-IVS-CsChrimson.mVenus Bloomington Drosophila Stock Center RRID:BDSC_55136
Genetic reagent (D. melanogaster) Drosophila: kdm5NP4707 Kyoto Stock Center; Hayashi et al., 2002 ; stock# 104754
Genetic reagent (D. melanogaster) Drosophila: kdm5140 Secombe Lab; Drelon et al., 2018
Genetic reagent (D. melanogaster) Drosophila: UASt-kdm5 Secombe Lab; Secombe et al., 2007
Genetic reagent (D. melanogaster) Drosophila: UAS-dMycRNAi Vienna Drosophila Resource Center Stock# KK106066
Genetic reagent (D. melanogaster) Drosophila: UAS-prosRNAi Bloomington Drosophila Stock Center RRID:BDSC_42538
Genetic reagent (D. melanogaster) Drosophila: tubP-Gal80ts Bloomington Drosophila Stock Center RRID:BDSC_7019
Genetic reagent (D. melanogaster) Drosophila: UAS-LT3-NDam Brand Lab; Southall et al., 2013
Genetic reagent (D. melanogaster) Drosophila: UAS-LT3-NDam-RpII215 Brand Lab; Southall et al., 2013
Genetic reagent (D. melanogaster) Drosophila: w1118 Bloomington Drosophila Stock Center RRID:BDSC_5905
Genetic reagent (D. melanogaster) Drosophila: UAS-LT3-NDam-kdm5 This study N/A Used for KDM5 TaDa (Figures 6 and 7). Available from lead contact.
Sequence-based reagent AdRt Vogel et al., 2007 PCR primers CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA
Sequence-based reagent AdRb Vogel et al., 2007 PCR primers TCCTCGGCCG
Sequence-based reagent DamID_PCR Vogel et al., 2007 PCR primers GGTCGCGGCCGAGGATC
Sequence-based reagent scram_shRNA This study PCR primers GGATAATAGAATAGTTATATTCAAGCATATTCTATTATCC
Sequence-based reagent Fw DsRed_KDM5_AarI This study PCR primers tatagtgtcttcggggccgaCAGGAGCTGTGGCGCATTCTAGAAAC
Sequence-based reagent PAM Rv This study PCR primers AATCTGGAACATCGTATGGGTACTGCGGCCGCGCTCGCGC
Sequence-based reagent PAM Fw This study PCR primers AGCAGCGGGCGGTGCAATCGGCGCGAGCGCGGCCGCAGTA
Sequence-based reagent Rv DsRed_KDM5_AarI This study PCR primers gattatctttctagggttaaAGGAAAAAGTCAAATAAAACGTAAGAAAACTTTGC
Sequence-based reagent Fw DsRed_KDM5_SapI This study PCR primers gactatctttctagggttaaTCAAAGGCGAAGGCGACTCT
Sequence-based reagent Rv DsRed_KDM5_SapI This study PCR primers atatggtcttcttttcccggAACATGTTCCTCTTTTAAGGTGCTCTTT
Sequence-based reagent Dam-kdm5_NotI-Fw This study PCR primers cgcagatctgcggccgATGTCCGCCAAAACTGAGG
Sequence-based reagent Dam-kdm5_XbaI-Rv This study PCR primers acaaagatcctctagCTACCGCGCCGATTGCAC
Recombinant DNA reagent pU6-BbsI-chiRNA Addgene; Gratz et al., 2013 RRID:Addgene_45946
Recombinant DNA reagent pValium20 Drosophila Genomics Resource Center; Ni et al., 2009 DGRC# 1467
Recombinant DNA reagent pHD-ScarlessDsRed Drosophila Genomics Resource Center DGRC# 1364
Software, algorithm Fiji https://fiji.sc/ RRID:SCR_002285
Software, algorithm Prism 6 GraphPad RRID:SCR_002798
Software, algorithm Cytoscape https://cytoscape.org/ RRID:SCR_003032
Software, algorithm Gene Ontology http://www.geneontology.org RRID:SCR_002811
Software, algorithm R 3.5.1 The R Foundation RRID:SCR_001905
Software, algorithm ggplot2 (R package) CRAN RRID:SCR_014601
Software, algorithm damidseq_pipeline Marshall and Brand, 2015a http://owenjm.github.io/damidseq_pipeline/
Software, algorithm find_peaks
Wolfram et al., 2012
http://github.com/owenjm/find_peaks
Software, algorithm bowtie2
Langmead and Salzberg, 2012
http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Software, algorithm BioVenn Hulsen et al., 2008 https://www.biovenn.nl/
Other Vectashield Mounting Medium Vector Labs Cat# H-1000
Other Normal donkey serum Fisher Scientific Cat# 50-413-367
Other DAPI-Fluoromount-G SouthernBiotech Cat# OB010020
Other T4 DNA ligase New England BioLabs Cat# M0202
Other T4 polynucleotide kinase New England BioLabs Cat# M0201
Other AarI Thermo Scientific Cat# ER1581
Other SapI New England BioLabs Cat# R0569
Other BpiI Thermo Scientific Cat# ER1011
Other NotI New England BioLabs Cat# R0189
Other PstI New England BioLabs Cat# R0140
Other NheI New England BioLabs Cat# R0131
Other EcoRI New England BioLabs Cat# R0101
Other DpnI​ New England BioLabs ​Cat# R0176
Other DpnI​I New England BioLabs Cat# R0543
Other AlwI​ New England BioLabs Cat# R0513

Funding Statement

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Contributor Information

Julie Secombe, Email: julie.secombe@einsteinmed.org.

Laura A Bradfield, University of Technology Sydney, Australia.

K VijayRaghavan, National Centre for Biological Sciences, Tata Institute of Fundamental Research, India.

Funding Information

This paper was supported by the following grants:

  • National Institutes of Health R01GM112783 to Julie Secombe.

  • National Health and Medical Research Council APP1128784 to Owen J Marshall.

  • National Health and Medical Research Council APP1185220 to Owen J Marshall.

  • National Institutes of Health F31NS110278 to Hayden AM Hatch.

  • National Institutes of Health T32GM007288 to Hayden AM Hatch.

  • Irma T. Hirschl Trust to Julie Secombe.

Additional information

Competing interests

No competing interests declared.

Author contributions

Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Writing - original draft, Writing - review and editing.

Resources, Validation, Writing - review and editing.

Conceptualization, Formal analysis, Supervision, Funding acquisition, Investigation, Writing - review and editing.

Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Writing - review and editing.

Additional files

Supplementary file 1. DEGs from kdm5140 TaDa.

Related to Figure 5.

elife-63886-supp1.xlsx (5.3MB, xlsx)
Supplementary file 2. GO categories from kdm5140 TaDa.

Related to Figure 5.

elife-63886-supp2.xlsx (25KB, xlsx)
Supplementary file 3. DEGs from kdm5 shRNA TaDa.

Related to Figure 5.

elife-63886-supp3.xlsx (5.3MB, xlsx)
Supplementary file 4. GO categories from kdm5 shRNA TaDa.

Related to Figure 5.

elife-63886-supp4.xlsx (19.3KB, xlsx)
Supplementary file 5. KDM5-regulated genes that are direct Dam-KDM5 targets.

Related to Figures 6 and 7.

elife-63886-supp5.xlsx (17.5KB, xlsx)
Supplementary file 6. KDM5-regulated genes that are direct Dam-Pros targets.

Related to Figure 7.

elife-63886-supp6.xlsx (17.1KB, xlsx)
Transparent reporting form

Data availability

TaDa data have been deposited in GEO under the accession codes GSE156010 and GSE166116.

The following datasets were generated:

Hatch HAM, Belalcazar HM, Marshall OJ, Secombe J. 2021. Targeted DamID analyses of neural progenitor cells and immature neurons of kdm5[140] and kdm5 shRNA Drosophila larvae and pupae. NCBI Gene Expression Omnibus. GSE156010

Hatch HAM, Belalcazar HM, Marshall OJ, Secombe J. 2021. Genome-wide binding profiles of KDM5 in Drosophila GMCs and immature neurons. NCBI Gene Expression Omnibus. GSE166116

The following previously published datasets were used:

Liu X, Shen J, Xie L, Wei Z, Wong C, Li Y, Zheng X, Li P, Song Y. 2020. Genome-wide binding profiles of HP1a and Prospero in Drosophila central brain neural precursors and neurons. NCBI Gene Expression Omnibus. GSE136413

Drelon C, Belalcazar B, Secombe J. 2018. RNA-seq analysis of kdm5 null mutant wing discs. NCBI Gene Expression Omnibus. GSE109201

References

  1. Androschuk A, Al-Jabri B, Bolduc FV. From learning to memory: what flies can tell us about intellectual disability treatment. Frontiers in Psychiatry. 2015;6:1–16. doi: 10.3389/fpsyt.2015.00085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aso Y, Hattori D, Yu Y, Johnston RM, Iyer NA, Ngo TT, Dionne H, Abbott LF, Axel R, Tanimoto H, Rubin GM. The neuronal architecture of the mushroom body provides a logic for associative learning. eLife. 2014;3:e04577. doi: 10.7554/eLife.04577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aughey GN, Estacio Gomez A, Thomson J, Yin H, Southall TD. CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo. eLife. 2018;7:e32341. doi: 10.7554/eLife.32341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Aughey GN, Cheetham SW, Southall TD. DamID as a versatile tool for understanding gene regulation. Development. 2019;146:dev173666. doi: 10.1242/dev.173666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bates KE, Sung CS, Robinow S. The unfulfilled gene is required for the development of mushroom body neuropil in Drosophila. Neural Development. 2010;5:4. doi: 10.1186/1749-8104-5-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Belalcazar HM, Hendricks EL, Zamurrad S, Liebl FLW, Secombe J. The histone demethylase KDM5 is required for synaptic structure and function at the Drosophila neuromuscular junction. Cell Reports. 2021;34:108753. doi: 10.1016/j.celrep.2021.108753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bellosta P, Gallant P. Myc function in Drosophila. Genes & Cancer. 2010;1:542–546. doi: 10.1177/1947601910377490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bhaumik SR, Smith E, Shilatifard A. Covalent modifications of histones during development and disease pathogenesis. Nature Structural & Molecular Biology. 2007;14:1008–1016. doi: 10.1038/nsmb1337. [DOI] [PubMed] [Google Scholar]
  9. Brookes E, Laurent B, Õunap K, Carroll R, Moeschler JB, Field M, Schwartz CE, Gecz J, Shi Y. Mutations in the intellectual disability gene KDM5C reduce protein stability and demethylase activity. Human Molecular Genetics. 2015;24:2861–2872. doi: 10.1093/hmg/ddv046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chen K, Luan X, Liu Q, Wang J, Chang X, Snijders AM, Mao JH, Secombe J, Dan Z, Chen JH, Wang Z, Dong X, Qiu C, Chang X, Zhang D, Celniker SE, Liu X. Drosophila histone demethylase KDM5 regulates social behavior through immune control and gut Microbiota maintenance. Cell Host & Microbe. 2019;25:537–552. doi: 10.1016/j.chom.2019.02.003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Choksi SP, Southall TD, Bossing T, Edoff K, de Wit E, Fischer BE, van Steensel B, Micklem G, Brand AH. Prospero acts as a binary switch between self-renewal and differentiation in Drosophila neural stem cells. Developmental Cell. 2006;11:775–789. doi: 10.1016/j.devcel.2006.09.015. [DOI] [PubMed] [Google Scholar]
  12. Doe CQ, Chu-LaGraff Q, Wright DM, Scott MP. The Prospero gene specifies cell fates in the Drosophila central nervous system. Cell. 1991;65:451–464. doi: 10.1016/0092-8674(91)90463-9. [DOI] [PubMed] [Google Scholar]
  13. Doupé DP, Marshall OJ, Dayton H, Brand AH, Perrimon N. Drosophila intestinal stem and progenitor cells are major sources and regulators of homeostatic niche signals. PNAS. 2018;115:12218–12223. doi: 10.1073/pnas.1719169115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Drelon C, Belalcazar HM, Secombe J. The histone demethylase KDM5 is essential for larval growth in Drosophila. Genetics. 2018;209:773–787. doi: 10.1534/genetics.118.301004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Drelon C, Rogers MF, Belalcazar HM, Secombe J. The histone demethylase KDM5 controls developmental timing in Drosophila by promoting prothoracic gland endocycles. Development. 2019;146:dev182568. doi: 10.1242/dev.182568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Dubnau J, Grady L, Kitamoto T, Tully T. Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memory. Nature. 2001;411:476–480. doi: 10.1038/35078077. [DOI] [PubMed] [Google Scholar]
  17. Elbert A, Bérubé NG. Chromatin structure and intellectual disability syndromes. Neuroscience and Biobehavioral Reviews. 2014;46:161–174. doi: 10.5772/55730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Froldi F, Szuperak M, Weng CF, Shi W, Papenfuss AT, Cheng LY. The transcription factor Nerfin-1 prevents reversion of neurons into neural stem cells. Genes & Development. 2015;29:129–143. doi: 10.1101/gad.250282.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Furukubo-Tokunaga K, Adachi Y, Kurusu M, Walldorf U. Brain patterning defects caused by mutations of the twin of eyeless gene in Drosophila melanogaster. Fly. 2009;3:263–269. doi: 10.4161/fly.10385. [DOI] [PubMed] [Google Scholar]
  20. Gajan A, Barnes VL, Liu M, Saha N, Pile LA. The histone demethylase dKDM5/LID interacts with the SIN3 histone deacetylase complex and shares functional similarities with SIN3. Epigenetics & Chromatin. 2016;9:1–23. doi: 10.1186/s13072-016-0053-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Gombos R, Migh E, Antal O, Mukherjee A, Jenny A, Mihaly J. The formin DAAM functions as molecular effector of the planar cell polarity pathway during axonal development in Drosophila. Journal of Neuroscience. 2015;35:10154–10167. doi: 10.1523/JNEUROSCI.3708-14.2015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Gratz SJ, Wildonger J, Harrison MM, O'Connor-Giles KM. CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand. Fly. 2013;7:249–255. doi: 10.4161/fly.26566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Greer EL, Shi Y. Histone methylation: a dynamic mark in health, disease and inheritance. Nature Reviews Genetics. 2012;13:343–357. doi: 10.1038/nrg3173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Grewal SS, Li L, Orian A, Eisenman RN, Edgar BA. Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development. Nature Cell Biology. 2005;7:295–302. doi: 10.1038/ncb1223. [DOI] [PubMed] [Google Scholar]
  25. Harmeyer KM, Facompre ND, Herlyn M, Basu D. JARID1 histone demethylases: emerging targets in Cancer. Trends in Cancer. 2017;3:713–725. doi: 10.1016/j.trecan.2017.08.004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Hattori D, Demir E, Kim HW, Viragh E, Zipursky SL, Dickson BJ. Dscam diversity is essential for neuronal wiring and self-recognition. Nature. 2007;449:223–227. doi: 10.1038/nature06099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Hayashi S, Ito K, Sado Y, Taniguchi M, Akimoto A, Takeuchi H, Aigaki T, Matsuzaki F, Nakagoshi H, Tanimura T, Ueda R, Uemura T, Yoshihara M, Goto S. GETDB, a database compiling expression patterns and molecular locations of a collection of Gal4 enhancer traps. Genesis. 2002;34:58–61. doi: 10.1002/gene.10137. [DOI] [PubMed] [Google Scholar]
  28. Heisenberg M, Borst A, Wagner S, Byers D. Drosophila mushroom body mutants are deficient in olfactory learning. Journal of Neurogenetics. 1985;2:1–30. doi: 10.3109/01677068509100140. [DOI] [PubMed] [Google Scholar]
  29. Henry GL, Davis FP, Picard S, Eddy SR. Cell type-specific genomics of Drosophila neurons. Nucleic Acids Research. 2012;40:9691–9704. doi: 10.1093/nar/gks671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Hulsen T, de Vlieg J, Alkema W. BioVenn - a web application for the comparison and visualization of biological lists using area-proportional venn diagrams. BMC Genomics. 2008;9:488. doi: 10.1186/1471-2164-9-488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Ito K, Hotta Y. Proliferation pattern of postembryonic neuroblasts in the brain of Drosophila melanogaster. Developmental Biology. 1992;149:134–148. doi: 10.1016/0012-1606(92)90270-Q. [DOI] [PubMed] [Google Scholar]
  32. Iwase S, Brookes E, Agarwal S, Badeaux AI, Ito H, Vallianatos CN, Tomassy GS, Kasza T, Lin G, Thompson A, Gu L, Kwan KY, Chen C, Sartor MA, Egan B, Xu J, Shi Y. A mouse model of X-linked intellectual disability associated with impaired removal of histone methylation. Cell Reports. 2016;14:1000–1009. doi: 10.1016/j.celrep.2015.12.091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Jenett A, Rubin GM, Ngo TT, Shepherd D, Murphy C, Dionne H, Pfeiffer BD, Cavallaro A, Hall D, Jeter J, Iyer N, Fetter D, Hausenfluck JH, Peng H, Trautman ET, Svirskas RR, Myers EW, Iwinski ZR, Aso Y, DePasquale GM, Enos A, Hulamm P, Lam SC, Li HH, Laverty TR, Long F, Qu L, Murphy SD, Rokicki K, Safford T, Shaw K, Simpson JH, Sowell A, Tae S, Yu Y, Zugates CT. A GAL4-driver line resource for Drosophila neurobiology. Cell Reports. 2012;2:991–1001. doi: 10.1016/j.celrep.2012.09.011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Kaltezioti V, Kouroupi G, Oikonomaki M, Mantouvalou E, Stergiopoulos A, Charonis A, Rohrer H, Matsas R, Politis PK. Prox1 regulates the notch1-mediated inhibition of neurogenesis. PLOS Biology. 2010;8:e1000565. doi: 10.1371/journal.pbio.1000565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Kelly SM, Bienkowski R, Banerjee A, Melicharek DJ, Brewer ZA, Marenda DR, Corbett AH, Moberg KH. The Drosophila ortholog of the Zc3h14 RNA binding protein acts within neurons to pattern axon projection in the developing brain. Developmental Neurobiology. 2016;76:93–106. doi: 10.1002/dneu.22301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Kong SY, Kim W, Lee HR, Kim HJ. The histone demethylase KDM5A is required for the repression of astrocytogenesis and regulated by the translational machinery in neural progenitor cells. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology. 2018;32:1108–1119. doi: 10.1096/fj.201700780R. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Kuzin A, Brody T, Moore AW, Odenwald WF. Nerfin-1 is required for early axon guidance decisions in the developing Drosophila CNS. Developmental Biology. 2005;277:347–365. doi: 10.1016/j.ydbio.2004.09.027. [DOI] [PubMed] [Google Scholar]
  38. Langmead B, Salzberg SL. Fast gapped-read alignment with bowtie 2. Nature Methods. 2012;9:357–359. doi: 10.1038/nmeth.1923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Lavado A, Lagutin OV, Chow LM, Baker SJ, Oliver G. Prox1 is required for granule cell maturation and intermediate progenitor maintenance during brain neurogenesis. PLOS Biology. 2010;8:e1000460. doi: 10.1371/journal.pbio.1000460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Lee MG, Norman J, Shilatifard A, Shiekhattar R. Physical and functional association of a trimethyl H3K4 demethylase and Ring6a/MBLR, a polycomb-like protein. Cell. 2007;128:877–887. doi: 10.1016/j.cell.2007.02.004. [DOI] [PubMed] [Google Scholar]
  41. Lee N, Erdjument-Bromage H, Tempst P, Jones RS, Zhang Y. The H3K4 demethylase lid associates with and inhibits histone deacetylase Rpd3. Molecular and Cellular Biology. 2009;29:1401–1410. doi: 10.1128/MCB.01643-08. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Leonard H, Wen X. The epidemiology of mental retardation: challenges and opportunities in the new millennium. Mental Retardation and Developmental Disabilities Research Reviews. 2002;8:117–134. doi: 10.1002/mrdd.10031. [DOI] [PubMed] [Google Scholar]
  43. Li G, Ruan X, Auerbach RK, Sandhu KS, Zheng M, Wang P, Poh HM, Goh Y, Lim J, Zhang J, Sim HS, Peh SQ, Mulawadi FH, Ong CT, Orlov YL, Hong S, Zhang Z, Landt S, Raha D, Euskirchen G, Wei CL, Ge W, Wang H, Davis C, Fisher-Aylor KI, Mortazavi A, Gerstein M, Gingeras T, Wold B, Sun Y, Fullwood MJ, Cheung E, Liu E, Sung WK, Snyder M, Ruan Y. Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation. Cell. 2012;148:84–98. doi: 10.1016/j.cell.2011.12.014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Li HH, Kroll JR, Lennox SM, Ogundeyi O, Jeter J, Depasquale G, Truman JW. A GAL4 driver resource for developmental and behavioral studies on the larval CNS of Drosophila. Cell Reports. 2014;8:897–908. doi: 10.1016/j.celrep.2014.06.065. [DOI] [PubMed] [Google Scholar]
  45. Liefke R, Oswald F, Alvarado C, Ferres-Marco D, Mittler G, Rodriguez P, Dominguez M, Borggrefe T. Histone demethylase KDM5A is an integral part of the core Notch-RBP-J repressor complex. Genes & Development. 2010;24:590–601. doi: 10.1101/gad.563210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Liu X, Greer C, Secombe J. KDM5 interacts with foxo to modulate cellular levels of oxidative stress. PLOS Genetics. 2014;10:e1004676. doi: 10.1371/journal.pgen.1004676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Liu X, Shen J, Xie L, Wei Z, Wong C, Li Y, Zheng X, Li P, Song Y. Mitotic implantation of the transcription factor Prospero via phase separation drives terminal neuronal differentiation. Developmental Cell. 2020;52:277–293. doi: 10.1016/j.devcel.2019.11.019. [DOI] [PubMed] [Google Scholar]
  48. Liu X, Secombe J. The histone demethylase KDM5 activates gene expression by recognizing chromatin context through its PHD reader motif. Cell Reports. 2015;13:2219–2231. doi: 10.1016/j.celrep.2015.11.007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Lloret-Llinares M, Pérez-Lluch S, Rossell D, Morán T, Ponsa-Cobas J, Auer H, Corominas M, Azorín F. dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes. Nucleic Acids Research. 2012;40:9493–9505. doi: 10.1093/nar/gks773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Lopez-Bigas N, Kisiel TA, Dewaal DC, Holmes KB, Volkert TL, Gupta S, Love J, Murray HL, Young RA, Benevolenskaya EV. Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation. Molecular Cell. 2008;31:520–530. doi: 10.1016/j.molcel.2008.08.004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Lussi YC, Mariani L, Friis C, Peltonen J, Myers TR, Krag C, Wong G, Salcini AE. Impaired removal of H3K4 methylation affects cell fate determination and gene transcription. Development. 2016;143:3751–3762. doi: 10.1242/dev.139139. [DOI] [PubMed] [Google Scholar]
  52. Marchetti G, Tavosanis G. Steroid hormone ecdysone signaling specifies mushroom body neuron sequential fate via chinmo. Current Biology. 2017;27:3017–3024. doi: 10.1016/j.cub.2017.08.037. [DOI] [PubMed] [Google Scholar]
  53. Mariani L, Lussi YC, Vandamme J, Riveiro A, Salcini AE. The H3K4me3/2 histone demethylase RBR-2 controls axon guidance by repressing the actin-remodeling gene wsp-1. Development. 2016;143:851–863. doi: 10.1242/dev.132985. [DOI] [PubMed] [Google Scholar]
  54. Marshall OJ, Brand AH, Southall TD, Gold KS, Egger B, Davidson CM, Caygill EE. polii.gene.call. ff3417dGitHub. 2013 https://github.com/owenjm/polii.gene.call
  55. Marshall OJ, Southall TD, Cheetham SW, Brand AH. Cell-type-specific profiling of protein-DNA interactions without cell isolation using targeted DamID with next-generation sequencing. Nature Protocols. 2016a;11:1586–1598. doi: 10.1038/nprot.2016.084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  56. Marshall OJ, Southall TD, Cheetham SW, Brand AH. find_peaks. 2259915GitHub. 2016b https://github.com/owenjm/find_peaks
  57. Marshall OJ, Brand AH. damidseq_pipeline: an automated pipeline for processing DamID sequencing datasets. Bioinformatics. 2015a;31:3371–3373. doi: 10.1093/bioinformatics/btv386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  58. Marshall OJ, Brand AH. damidseq_pipeline. 21fb45fGitHub. 2015b https://github.com/owenjm/damidseq_pipeline
  59. Marshall OJ, Brand AH. Chromatin state changes during neural development revealed by in vivo cell-type specific profiling. Nature Communications. 2017;8:1–9. doi: 10.1038/s41467-017-02385-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  60. Mi H, Muruganujan A, Casagrande JT, Thomas PD. Large-scale gene function analysis with the panther classification system. Nature Protocols. 2013;8:1551–1566. doi: 10.1038/nprot.2013.092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  61. Michel CI, Kraft R, Restifo LL. Defective neuronal development in the mushroom bodies of Drosophila fragile X mental retardation 1 mutants. Journal of Neuroscience. 2004;24:5798–5809. doi: 10.1523/JNEUROSCI.1102-04.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  62. Miyoshi G, Young A, Petros T, Karayannis T, McKenzie Chang M, Lavado A, Iwano T, Nakajima M, Taniguchi H, Huang ZJ, Heintz N, Oliver G, Matsuzaki F, Machold RP, Fishell G. Prox1 regulates the Subtype-Specific development of caudal ganglionic Eminence-Derived GABAergic cortical interneurons. Journal of Neuroscience. 2015;35:12869–12889. doi: 10.1523/JNEUROSCI.1164-15.2015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  63. Morris JH, Apeltsin L, Newman AM, Baumbach J, Wittkop T, Su G, Bader GD, Ferrin TE. clusterMaker: a multi-algorithm clustering Plugin for cytoscape. BMC Bioinformatics. 2011;12:1–14. doi: 10.1186/1471-2105-12-436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  64. Navarro-Costa P, McCarthy A, Prudêncio P, Greer C, Guilgur LG, Becker JD, Secombe J, Rangan P, Martinho RG. Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling. Nature Communications. 2016;7:12331. doi: 10.1038/ncomms12331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  65. Ni JQ, Liu LP, Binari R, Hardy R, Shim HS, Cavallaro A, Booker M, Pfeiffer BD, Markstein M, Wang H, Villalta C, Laverty TR, Perkins LA, Perrimon N. A Drosophila resource of transgenic RNAi lines for neurogenetics. Genetics. 2009;182:1089–1189. doi: 10.1534/genetics.109.103630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Nishibuchi G, Shibata Y, Hayakawa T, Hayakawa N, Ohtani Y, Sinmyozu K, Tagami H, Nakayama J. Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. Journal of Biological Chemistry. 2014;289:28956–28970. doi: 10.1074/jbc.M114.573725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  67. Park S, Kim GW, Kwon SH, Lee JS. Broad domains of histone H3 lysine 4 trimethylation in transcriptional regulation and disease. The FEBS Journal. 2020;287:2891–2902. doi: 10.1111/febs.15219. [DOI] [PubMed] [Google Scholar]
  68. Parkel S, Lopez-Atalaya JP, Barco A. Histone H3 lysine methylation in cognition and intellectual disability disorders. Learning & Memory. 2013;20:570–579. doi: 10.1101/lm.029363.112. [DOI] [PubMed] [Google Scholar]
  69. Pfeiffer BD, Jenett A, Hammonds AS, Ngo TT, Misra S, Murphy C, Scully A, Carlson JW, Wan KH, Laverty TR, Mungall C, Svirskas R, Kadonaga JT, Doe CQ, Eisen MB, Celniker SE, Rubin GM. Tools for neuroanatomy and neurogenetics in Drosophila. PNAS. 2008;105:9715–9720. doi: 10.1073/pnas.0803697105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  70. Quinlan AR, Hall IM. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics. 2010;26:841–842. doi: 10.1093/bioinformatics/btq033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  71. Ropers HH. Genetics of early onset cognitive impairment. Annual Review of Genomics and Human Genetics. 2010;11:161–187. doi: 10.1146/annurev-genom-082509-141640. [DOI] [PubMed] [Google Scholar]
  72. Scandaglia M, Lopez-Atalaya JP, Medrano-Fernandez A, Lopez-Cascales MT, Del Blanco B, Lipinski M, Benito E, Olivares R, Iwase S, Shi Y, Barco A. Loss of Kdm5c causes spurious transcription and prevents the Fine-Tuning of Activity-Regulated enhancers in neurons. Cell Reports. 2017;21:47–59. doi: 10.1016/j.celrep.2017.09.014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  73. Secombe J, Li L, Carlos L, Eisenman RN. The trithorax group protein lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth. Genes & Development. 2007;21:537–551. doi: 10.1101/gad.1523007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  74. Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B, Ideker T. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Research. 2003;13:2498–2504. doi: 10.1101/gr.1239303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  75. Southall TD, Gold KS, Egger B, Davidson CM, Caygill EE, Marshall OJ, Brand AH. Cell-type-specific profiling of gene expression and chromatin binding without cell isolation: assaying RNA pol II occupancy in neural stem cells. Developmental Cell. 2013;26:101–112. doi: 10.1016/j.devcel.2013.05.020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  76. Stempor P, Ahringer J. SeqPlots - Interactive software for exploratory data analyses, pattern discovery and visualization in genomics. Wellcome Open Research. 2016;1:14. doi: 10.12688/wellcomeopenres.10004.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  77. Syed MH, Mark B, Doe CQ. Steroid hormone induction of temporal gene expression in Drosophila brain neuroblasts generates neuronal and glial diversity. eLife. 2017;6:e26287. doi: 10.7554/eLife.26287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  78. Tarazona S, García-Alcalde F, Dopazo J, Ferrer A, Conesa A. Differential expression in RNA-seq: a matter of depth. Genome Research. 2011;21:2213–2223. doi: 10.1101/gr.124321.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  79. Tea JS, Chihara T, Luo L. Histone deacetylase Rpd3 regulates olfactory projection neuron dendrite targeting via the transcription factor Prospero. Journal of Neuroscience. 2010;30:9939–9946. doi: 10.1523/JNEUROSCI.1643-10.2010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  80. Technau G, Heisenberg M. Neural reorganization during metamorphosis of the corpora pedunculata in Drosophila melanogaster. Nature. 1982;295:405–407. doi: 10.1038/295405a0. [DOI] [PubMed] [Google Scholar]
  81. The Gene Ontology Consortium Gene ontology: tool for the unification of biology. Gene Expression. 2000;25:25–29. doi: 10.1038/75556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  82. The Gene Ontology Consortium The gene ontology resource: 20 years and still GOing strong. Nucleic Acids Research. 2019;47:D330–D338. doi: 10.1093/nar/gky1055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  83. Thurman RE, Rynes E, Humbert R, Vierstra J, Maurano MT, Haugen E, Sheffield NC, Stergachis AB, Wang H, Vernot B, Garg K, John S, Sandstrom R, Bates D, Boatman L, Canfield TK, Diegel M, Dunn D, Ebersol AK, Frum T, Giste E, Johnson AK, Johnson EM, Kutyavin T, Lajoie B, Lee BK, Lee K, London D, Lotakis D, Neph S, Neri F, Nguyen ED, Qu H, Reynolds AP, Roach V, Safi A, Sanchez ME, Sanyal A, Shafer A, Simon JM, Song L, Vong S, Weaver M, Yan Y, Zhang Z, Zhang Z, Lenhard B, Tewari M, Dorschner MO, Hansen RS, Navas PA, Stamatoyannopoulos G, Iyer VR, Lieb JD, Sunyaev SR, Akey JM, Sabo PJ, Kaul R, Furey TS, Dekker J, Crawford GE, Stamatoyannopoulos JA. The accessible chromatin landscape of the human genome. Nature. 2012;489:75–82. doi: 10.1038/nature11232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  84. Truman JW, Bate M. Spatial and temporal patterns of neurogenesis in the central nervous system of Drosophila melanogaster. Developmental Biology. 1988;125:145–157. doi: 10.1016/0012-1606(88)90067-X. [DOI] [PubMed] [Google Scholar]
  85. Vaessin H, Grell E, Wolff E, Bier E, Jan LY, Jan YN. Prospero is expressed in neuronal precursors and encodes a nuclear protein that is involved in the control of axonal outgrowth in Drosophila. Cell. 1991;67:941–953. doi: 10.1016/0092-8674(91)90367-8. [DOI] [PubMed] [Google Scholar]
  86. Vallianatos CN, Farrehi C, Friez MJ, Burmeister M, Keegan CE, Iwase S. Altered Gene-Regulatory function of KDM5C by a novel mutation associated with autism and intellectual disability. Frontiers in Molecular Neuroscience. 2018;11:1–12. doi: 10.3389/fnmol.2018.00104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  87. Vallianatos CN, Raines B, Porter RS, Bonefas KM, Wu MC, Garay PM, Collette KM, Seo YA, Dou Y, Keegan CE, Tronson NC, Iwase S. Mutually suppressive roles of KMT2A and KDM5C in behaviour, neuronal structure, and histone H3K4 methylation. Communications Biology. 2020;3:278. doi: 10.1038/s42003-020-1001-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  88. Vallianatos CN, Iwase S. Disrupted intricacy of histone H3K4 methylation in neurodevelopmental disorders. Epigenomics. 2015;7:503–519. doi: 10.2217/epi.15.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  89. van Bokhoven H. Genetic and epigenetic networks in intellectual disabilities. Annual Review of Genetics. 2011;45:81–104. doi: 10.1146/annurev-genet-110410-132512. [DOI] [PubMed] [Google Scholar]
  90. van Oevelen C, Wang J, Asp P, Yan Q, Kaelin WG, Kluger Y, Dynlacht BD. A role for mammalian Sin3 in permanent gene silencing. Molecular Cell. 2008;32:359–370. doi: 10.1016/j.molcel.2008.10.015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  91. Vogel MJ, Peric-Hupkes D, van Steensel B. Detection of in vivo protein-DNA interactions using DamID in mammalian cells. Nature Protocols. 2007;2:1467–1478. doi: 10.1038/nprot.2007.148. [DOI] [PubMed] [Google Scholar]
  92. Wei G, Deng X, Agarwal S, Iwase S, Disteche C, Xu J. Patient mutations of the intellectual disability gene KDM5C downregulate netrin G2 and suppress neurite growth in Neuro2a cells. Journal of Molecular Neuroscience. 2016;60:33–45. doi: 10.1007/s12031-016-0770-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  93. Witteveen JS, Willemsen MH, Dombroski TCD, van Bakel NHM, Nillesen WM, van Hulten JA, Jansen EJR, Verkaik D, Veenstra-Knol HE, van Ravenswaaij-Arts CMA, Wassink-Ruiter JSK, Vincent M, David A, Le Caignec C, Schieving J, Gilissen C, Foulds N, Rump P, Strom T, Cremer K, Zink AM, Engels H, de Munnik SA, Visser JE, Brunner HG, Martens GJM, Pfundt R, Kleefstra T, Kolk SM. Haploinsufficiency of MeCP2-interacting transcriptional co-repressor SIN3A causes mild intellectual disability by affecting the development of cortical integrity. Nature Genetics. 2016;48:877–887. doi: 10.1038/ng.3619. [DOI] [PubMed] [Google Scholar]
  94. Wolfram V, Southall TD, Brand AH, Baines RA. The LIM-homeodomain protein islet dictates motor neuron electrical properties by regulating K(+) channel expression. Neuron. 2012;75:663–674. doi: 10.1016/j.neuron.2012.06.015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  95. Yuan L, Hu S, Okray Z, Ren X, De Geest N, Claeys A, Yan J, Bellefroid E, Hassan BA, Quan XJ. The Drosophila neurogenin tap functionally interacts with the Wnt-PCP pathway to regulate neuronal extension and guidance. Development. 2016;143:2760–2766. doi: 10.1242/dev.134155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  96. Zamurrad S, Hatch HAM, Drelon C, Belalcazar HM, Secombe J. A Drosophila model of intellectual disability caused by mutations in the histone demethylase KDM5. Cell Reports. 2018;22:2359–2369. doi: 10.1016/j.celrep.2018.02.018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  97. Zhan XL, Clemens JC, Neves G, Hattori D, Flanagan JJ, Hummel T, Vasconcelos ML, Chess A, Zipursky SL. Analysis of dscam diversity in regulating axon guidance in Drosophila mushroom bodies. Neuron. 2004;43:673–686. doi: 10.1016/j.neuron.2004.07.020. [DOI] [PubMed] [Google Scholar]

Decision letter

Editor: Laura A Bradfield1
Reviewed by: Shigeki Iwase2

In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.

Acceptance summary:

This study uses Drosophila to examine KDM5, histone H3K4 demethylase, that has been implicated in intellectual disabilities. The results show how KDM5 in the mushroom body is required for axonal development, independently of its histone demethylase activity. Insights revealed by this study include how KDM5 is involved in the growth and development of ganglion mother cells and immature neurons.

Decision letter after peer review:

Thank you for submitting your article "A KDM5-Prospero transcriptional axis functions during early neurodevelopment to regulate mushroom body formation" for consideration by eLife. Your article has been reviewed by three peer reviewers, and the evaluation has been overseen by a Reviewing Editor and K VijayRaghavan as the Senior Editor.

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

As the editors have judged that your manuscript is of interest, but as described below that additional experiments or substantial rewriting are required before it is published, we would like to draw your attention to changes in our revision policy that we have made in response to COVID-19 (https://elifesciences.org/articles/57162). First, because many researchers have temporarily lost access to the labs, we will give authors as much time as they need to submit revised manuscripts. We are also offering, if you choose, to post the manuscript to bioRxiv (if it is not already there) along with this decision letter and a formal designation that the manuscript is "in revision at eLife". Please let us know if you would like to pursue this option. (If your work is more suitable for medRxiv, you will need to post the preprint yourself, as the mechanisms for us to do so are still in development.)

Summary:

Overall the reviews of your manuscript were mixed, with two reviewers very positive, and one reviewer with several important comments that need to be addressed. Nevertheless, the consensus was that the manuscript is sufficiently interesting, well written, and novel enough to warrant the consideration of a revised version.

Reviewer #3 in particular had several concerns that would either require either more experiments, or the re-focusing of the central story of the manuscript. I will leave it to the authors to decide which option to take.

Essential revisions:

The suggested extra experiments are:

1) Perform their mutant analysis with entirely different genetics: Making these lines will take time – although maybe since the lab has studied the role of KDM5 in many biological settings they have these lines already?

2) Perform their Tada and behaviour analyses with more specific Gal4 lines, if developmentally expressed, MB-specific Gal4 lines exist.

3) Perform more genetic epistatic experiments with pros.

If the authors prefer to re-focus their story, however, this is the specific suggestion from reviewer #3:

"In my opinion, what's interesting is that different domains of KDM5 have different functions. In their 2018 paper (Zamurrad et al., 2018) they generated fly lines carrying mutations linked with ID in humans. These lines showed no neuroanatomical defects in the mushroom body but had defects in short and long term memory.

In this paper that we're discussing, the authors focus on a mutation that affects a different part of the gene. This, they find, causes severe neuroanatomical defects in the MB (probably elsewhere too), likely through its interaction with prosperso, a potent transcriptional regulator. This is interesting! I would urge the authors to make this the centerpiece of their story:

1) Thoroughly describe the cellular mechanism of the phenotype.

2) Thoroughly establish the KDM5>pros genetic link.

3) Describe the transcriptional programme that is affected.

If they chose to do this, I don't think that they need to focus on the mushroom body – both pros and KDM5 are ubiquitously expressed. This way, I also think that the behaviour experiments are not necessary. And finally, this way, the authors need not revisit their Tada experiments. They could focus only on getting the genetics of the cellular phenotypes and the pros interaction going and on re-focussing the text."

The specific reviews are appended:

Reviewer #1:

In this paper, Hatch et al. describe how KDM5 functions within the developing adult Drosophila brain to control normal formation of the mushroom body (MB). They show that loss of KDM5 functions within specific neural progenitor cells leads to defects in neuronal guidance and growth within the MB, and leads to disrupted cognitive function. Using a nice combination of whole-genome expression profiling approaches, they identify the gene targets regulated by KDM5 with neural progenitor cells. In particular they identify Prospero as a key downstream mediator of KDM5 function in the MB.

Overall, I liked this paper a lot. The experiments are all well performed, the paper is well written, and, most importantly, the discoveries should be of interest to a broad group of developmental and behavioural neurobiologists.

Reviewer #2:

The work describes the first cell-type-specific analysis of histone H3K4 demethylase KDM5 in fly brain development. The experiments and analyses appear to be well designed and executed. The data support most of the claims. The authors used the state-of-the-art genomic profiling assays for pol II binding and chromatin accessibility. An important finding is the striking overlap of pro occupancy and KDM5-regulated genes, which is backed up by the robust genetic data demonstrating the functional interaction of the two genes. Mutations in multiple KDM5-family enzymes are responsible for human neurodevelopmental disorders. Thus, the work is an essential step towards understanding the pathophysiology of the conditions.

Reviewer #3:

In this manuscript the authors study the role gene histone demethylase gene, KDM5, in the development of the mushroom body. In vertebrates, loss of function of homologues of this gene have been shown to be involved in intellectual disabilities. The authors generate a tagged form of the protein to assess its expression pattern and find it to be expressed ubiquitously in the brain. They show that a loss of this gene results in dramatic defects in the mushroom body and use a combination of Gal4 lines to narrow down which cells it might be required in – stem cells, intermediate precursors and immature neurons. They profile these brains transcriptionally and identify targets that share >40% overlap with Pros target genes. They test a possible interaction KDM5 and pros through a transheterozygous interaction between the two genes.

The manuscript is generally well written and presented.

[Editors' note: further revisions were suggested prior to acceptance, as described below.]

Thank you for submitting your article "A KDM5-Prospero transcriptional axis functions during early neurodevelopment to regulate mushroom body formation" for consideration by eLife. Your article has been reviewed by three peer reviewers, and the evaluation has been overseen by a Reviewing Editor and K VijayRaghavan as the Senior Editor. The reviewers have opted to remain anonymous.

The reviewers have discussed their reviews with one another, and the Reviewing Editor has drafted this to help you prepare a revised submission.

Essential Revisions:

Please see the revisions suggested by reviewer #3. Upon consultation, it was decided that none of these revisions are necessarily essential, but if the authors could make some efforts to 1) increase cellular resolution analysis, and 2) strengthen the KDM5-pros link, in the manner described below. If this is not feasible for any reason please let us know.

Reviewer #1:

This paper uses a nice combination of genetics and whole-genome investigation of transcription to reveal a kdm5-pros transcriptional module that is important for regulating neuronal gen expression and for controlling development of the mushroom body. Given that both kdm5 and pros are conserved, these findings may be important for our understanding of neuronal gene expression in other contexts.

In this revised manuscript, the authors provide new data that strengthens the original paper. I particularly liked the new kdm5 Dam ID experiments showing kdm5 localization at the pros gene – these results, along with the original genetic epistasis experiments, emphasize an important kdm5-pros transcriptional network in the control of MB development.

The authors have satisfactorily addressed all my original comments and I am happy to accept the paper for publication in eLife. Congratulations to the authors on a very nice piece of work

Reviewer #2:

The work describes the first cell-type-specific analysis of histone H3K4 demethylase KDM5 in fly brain development. The experiments and analyses appear to be well designed and executed. The data support most of the claims. The authors used the state-of-the-art genomic profiling assays for pol II binding and chromatin accessibility. An important finding is the striking overlap of pro occupancy and KDM5-regulated genes, which is backed up by the robust genetic data demonstrating the functional interaction of the two genes. Furthermore, the authors were able to show pro is a direct target gene KDM5C regulates in a specific neuron type of the fly brain. Mutations in multiple KDM5-family enzymes are responsible for human neurodevelopmental disorders. Thus, the work is an essential step towards understanding the pathophysiology of the conditions.

The authors addressed comments from the reviewers well. It is nice to see that KDM5C directly regulates the expression of pro rather than the two factors work in concert.

Reviewer #3:

In this manuscript the authors study the role of a histone demethylase gene, KDM5, in the development of the mushroom body. In vertebrates, loss of function of homologues of this gene have been shown to be involved in intellectual disabilities. The authors generate a tagged form of the protein to assess its expression pattern and find it to be expressed ubiquitously in the brain. They show that a loss of this gene results in dramatic defects in the mushroom body and use a combination of Gal4 lines to narrow down which cells it might be required in – stem cells, intermediate precursors and immature neurons. They profile these brains transcriptionally, as well profile KDM5 binding in these brains. They find that KDM5 binds to the pros locus, whose transcript is down regulated in the absence of KDM5. Interestingly they show that this is not due to KDM5's role in chromatin regulation. They find a significant overlap in the targets of KDM5 and pros and postulate that the two act together in growth and patterning. They test a possible interaction between KDM5 and pros through a transheterozygous interactions between the two genes in MB patterning.

While interesting, I have some concerns that I am listing below.

My major concern with this manuscript is the resolution of analysis. The authors analyze mutant phenotypes and assign cell-specific requirements based on Gal4 drivers and their previously ascribed cell specificities. A more thorough approach would have been to assess the requirement of KDM5 in clonal analysis that irrefutably manipulates the function of KDM5 in these specific cell types and then assess their effects at a cellular level.

The authors profile the transcriptome of KDM5 depleted GMCs and neurons using the broadly expressed R71C09-Gal4. Similarly, they assay for KDM5 binding in the R71C09-Gal4 domain. As the knockdown of KDM5 is also dependent on the Gal4 (or is a whole animal mutant), in my view, the authors are studying these regulatory processes and interactions in all NBs and GMCs. They however interpret these data as being specific to the mushroom body due to the timing of the experiments. I do not understand the reasoning behind this interpretation.

I find the data interesting. I believe the authors have uncovered a general process by which neurons mature and form connections as both pros and KDM5 are ubiquitously expressed. This will be of interest – as will the datasets that have been generated in this study – to the field in general.

I think this revised manuscript is much better without the behaviour. However, two of my earlier concerns still remain unaddressed. I'll reiterate them here:

1) I would recommend improved cellular resolution of their analysis. This could be done by performing clonal analysis to show the specific cell type in which KDM5 is required. The authors could use the MB as an illustrative cell-type, but I don't think it necessary – KDM5 is ubiquitously expressed and their accompanying TaDa analysis has been done with the broadly expressed R71C09-Gal4. The authors could take this decision based on whatever genetics is easier done. Such clonal analysis will also give the authors far better cellular resolution of their phenotypes.

2) Strengthen their KDM5-pros link. In this revised manuscript, the authors show that KDM5 binds pros, but the binding seems barely above background (was it picked up in a peak call?). They also say that no physical interaction was picked up between the two in IP experiments. So, if this is central to their story, I would recommend they build this axis with more convincing data. For example, if KDM5 acts through pros, is pros protein downregulated in the KDM5 null? (There are very good pros antibodies out there.). Can Pros rescue KDM5 mutant phenotype?

eLife. 2021 Mar 17;10:e63886. doi: 10.7554/eLife.63886.sa2

Author response


Essential revisions:

The suggested extra experiments are:

1) Perform their mutant analysis with entirely different genetics: Making these lines will take time – although maybe since the lab has studied the role of KDM5 in many biological settings they have these lines already?

2) Perform their Tada and behaviour analyses with more specific Gal4 lines, if developmentally expressed, MB-specific Gal4 lines exist.

3) Perform more genetic epistatic experiments with pros.

If the authors prefer to re-focus their story, however, this is the specific suggestion from reviewer #3:

"In my opinion, what's interesting is that different domains of KDM5 have different functions. In their 2018 paper (Zamurrad et al., 2018) they generated fly lines carrying mutations linked with ID in humans. These lines showed no neuroanatomical defects in the mushroom body but had defects in short and long term memory.

In this paper that we're discussing, the authors focus on a mutation that affects a different part of the gene. This, they find, causes severe neuroanatomical defects in the MB (probably elsewhere too), likely through its interaction with prosperso, a potent transcriptional regulator. This is interesting! I would urge the authors to make this the centerpiece of their story:

1) Thoroughly describe the cellular mechanism of the phenotype.

2) Thoroughly establish the KDM5>pros genetic link.

3) Describe the transcriptional programme that is affected.

If they chose to do this, I don't think that they need to focus on the mushroom body – both pros and KDM5 are ubiquitously expressed. This way, I also think that the behaviour experiments are not necessary. And finally, this way, the authors need not revisit their Tada experiments. They could focus only on getting the genetics of the cellular phenotypes and the pros interaction going and on re-focussing the text."

In response to editor and reviewer suggestions, we have included data which significantly strengthen our model linking KDM5 and Prospero (Figures 6,7; Supplementary files 5,6). Specifically, we generated a fly strain expressing a UAS-dam-kdm5 transgene under control of the R71C09-Gal4 driver. This allowed us to assess direct genetic targets of KDM5 within immature neurons and GMCs during a similar developmental window as for our Pol II TaDa datasets. Not only does this represent the first cell-specific analysis of KDM5 occupancy, but it has allowed us to demonstrate that KDM5 directly binds to pros at transcriptional start sites to regulate its expression (Figure 6B-D; Figure 7A-C). This additional data provide clear support for our model that a KDM5-Pros transcriptional axis is critically important for mushroom body development.

[Editors' note: further revisions were suggested prior to acceptance, as described below.]

Essential Revisions:

Please see the revisions suggested by reviewer #3. Upon consultation, it was decided that none of these revisions are necessarily essential, but if the authors could make some efforts to 1) increase cellular resolution analysis, and 2) strengthen the KDM5-pros link, in the manner described below. If this is not feasible for any reason please let us know.

Reviewer #3:

[…]

I think this revised manuscript is much better without the behaviour. However, two of my earlier concerns still remain unaddressed. I'll reiterate them here:

1) I would recommend improved cellular resolution of their analysis. This could be done by performing clonal analysis to show the specific cell type in which KDM5 is required. The authors could use the MB as an illustrative cell-type, but I don't think it necessary – KDM5 is ubiquitously expressed and their accompanying TaDa analysis has been done with the broadly expressed R71C09-Gal4. The authors could take this decision based on whatever genetics is easier done. Such clonal analysis will also give the authors far better cellular resolution of their phenotypes.

It is unclear from reviewer 3’s comment what significant new information examining the effects of kdm5 loss at a single cell level would provide to our investigation. Our current cell type-specific knockdown data already convincingly demonstrate that KDM5 is required within ganglion mother cells (GMCs) and immature neurons for proper axonal growth and guidance. While the 71C09-Gal4 driver is expressed in many GMCs within the brain, we chose to focus our analyses on the Mushroom Body due to its highly characterized morphology and links to cognitive function. We do, however, note in text that depletion of KDM5 using this Gal4 driver could lead to defects within other neuronal structures, the characterization of which are outside the scope of this manuscript.

2) Strengthen their KDM5-pros link. In this revised manuscript, the authors show that KDM5 binds pros, but the binding seems barely above background (was it picked up in a peak call?). They also say that no physical interaction was picked up between the two in IP experiments. So, if this is central to their story, I would recommend they build this axis with more convincing data. For example, if KDM5 acts through pros, is pros protein downregulated in the KDM5 null? (There are very good pros antibodies out there.). Can Pros rescue KDM5 mutant phenotype?

Regarding the Dam:KDM5 TaDa binding data, it is possible that reviewer 3 is more familiar with looking at ChIP-seq genome browser tracks and not those generated by TaDa analyses. As described in detail in the Materials and methods section, Dam:KDM5 binding at the pros gene was significant when compared to expression of Dam alone (using a 1% FDR cutoff and six biological replicates). To further clarify this matter, we now show the two peaks called within the pros gene in Figure 7, which have p(adj) values of 0.00052 and 0.00363, respectively. A file containing all significant called peaks, along with p(adj) values has been added to the referenced GEO submission.

Reviewer 3’s second concern relates to whether levels of Pros protein are affected by KDM5 depletion as assessed via IHC. We have performed IHC staining of pupal and adult brains using two independent Pros antibodies, one of which was obtained from Drs. Lily and Yuh-Nung Jan and the other from the Developmental Studies Hybridoma Bank. Although these antibodies work well in embryonic and larval brain tissue, they do not clearly detect Pros protein within pupal and adult tissue. The reason for this is unclear but may be related to the fact that these antibodies do not detect all Pros isoforms.

Reviewer 3’s third concern relates to whether overexpression of Pros can rescue the kdm5140 mutant (or knockdown) phenotype. We obtained a UAS-Pros transgene from the Bloomington Drosophila Stock Center to carry out this experiment. Unfortunately, overexpression of Pros using R71C09-Gal4 kills animals during early embryonic and larval development. Additionally, overexpression of Pros within Kenyon cells using OK107-Gal4 results in complete ablation of the mushroom body. This toxicity prevented us from performing the suggested rescue experiments.

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Data Citations

    1. Hatch HAM, Belalcazar HM, Marshall OJ, Secombe J. 2021. Targeted DamID analyses of neural progenitor cells and immature neurons of kdm5[140] and kdm5 shRNA Drosophila larvae and pupae. NCBI Gene Expression Omnibus. GSE156010
    2. Hatch HAM, Belalcazar HM, Marshall OJ, Secombe J. 2021. Genome-wide binding profiles of KDM5 in Drosophila GMCs and immature neurons. NCBI Gene Expression Omnibus. GSE166116
    3. Liu X, Shen J, Xie L, Wei Z, Wong C, Li Y, Zheng X, Li P, Song Y. 2020. Genome-wide binding profiles of HP1a and Prospero in Drosophila central brain neural precursors and neurons. NCBI Gene Expression Omnibus. GSE136413
    4. Drelon C, Belalcazar B, Secombe J. 2018. RNA-seq analysis of kdm5 null mutant wing discs. NCBI Gene Expression Omnibus. GSE109201

    Supplementary Materials

    Supplementary file 1. DEGs from kdm5140 TaDa.

    Related to Figure 5.

    elife-63886-supp1.xlsx (5.3MB, xlsx)
    Supplementary file 2. GO categories from kdm5140 TaDa.

    Related to Figure 5.

    elife-63886-supp2.xlsx (25KB, xlsx)
    Supplementary file 3. DEGs from kdm5 shRNA TaDa.

    Related to Figure 5.

    elife-63886-supp3.xlsx (5.3MB, xlsx)
    Supplementary file 4. GO categories from kdm5 shRNA TaDa.

    Related to Figure 5.

    elife-63886-supp4.xlsx (19.3KB, xlsx)
    Supplementary file 5. KDM5-regulated genes that are direct Dam-KDM5 targets.

    Related to Figures 6 and 7.

    elife-63886-supp5.xlsx (17.5KB, xlsx)
    Supplementary file 6. KDM5-regulated genes that are direct Dam-Pros targets.

    Related to Figure 7.

    elife-63886-supp6.xlsx (17.1KB, xlsx)
    Transparent reporting form

    Data Availability Statement

    TaDa data have been deposited in GEO under the accession codes GSE156010 and GSE166116.

    The following datasets were generated:

    Hatch HAM, Belalcazar HM, Marshall OJ, Secombe J. 2021. Targeted DamID analyses of neural progenitor cells and immature neurons of kdm5[140] and kdm5 shRNA Drosophila larvae and pupae. NCBI Gene Expression Omnibus. GSE156010

    Hatch HAM, Belalcazar HM, Marshall OJ, Secombe J. 2021. Genome-wide binding profiles of KDM5 in Drosophila GMCs and immature neurons. NCBI Gene Expression Omnibus. GSE166116

    The following previously published datasets were used:

    Liu X, Shen J, Xie L, Wei Z, Wong C, Li Y, Zheng X, Li P, Song Y. 2020. Genome-wide binding profiles of HP1a and Prospero in Drosophila central brain neural precursors and neurons. NCBI Gene Expression Omnibus. GSE136413

    Drelon C, Belalcazar B, Secombe J. 2018. RNA-seq analysis of kdm5 null mutant wing discs. NCBI Gene Expression Omnibus. GSE109201


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