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. 2021 Mar 4;10(3):530. doi: 10.3390/foods10030530

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of dietary treatments on genes are associated with barrier function damage in colon. MUC-2 and ECM1 gene expressions were evaluated in the colon of DSS-treated piglets by qPCR. The expression of target gene was normalized to the geometric mean of two reference genes; the results, expressed as fold changes (Fc) are presented as mean ± SEM. Values are the means from two independent replicates of colon samples (n = 5/group), with their standard errors represented by vertical bars. ^a,b Groups with unlike superscript letters were significantly different (p < 0.05; one-way ANOVA followed by Fisher test).