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Construction of the Lycium barbarum polysaccharide (LBP) metabolic pathways in Saccharomyces cerevisiae through extraction of total RNA from different tissues of goji berry, analyzing the different expression strength in different parts. Using HMM and gene similarity network to analyze the predicted key enzymes, optimize the codons of the key enzyme would lead to the LBP production in engineered S. cerevisiae using synthetic biology strategies.
