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. 2021 Mar 17;13(3):493. doi: 10.3390/v13030493

Figure 6.

Figure 6

Chimeric CrPV-aNCV clone is infectious in Drosophila S2 cells. (A) Schematic of the chimeric CrPV-aNCV clone replacing CrPV IGR IRES with that of the aNCV IRES. (B) In vitro translation of CrPV and CrPV-aNCV RNAs in Sf21 extracts. CrPV-ORF1-STOP contains a stop codon within the N-terminal ORF1, thus preventing expression of the non-structural proteins. Reactions were resolved by SDS-PAGE and visualized by autoradiography. (C) Immunoblotting of CrPV VP2 structural protein and 1A non-structural protein. (D) RT-PCR of viral negative-strand RNA from Drosophila S2 cells transfected with the indicated viral clone RNAs at 72 and 144 h after transfection. (E) Immunoblotting of CrPV 1A and VP2 proteins from lysates of Drosophila S2 cells infected with CrPV or CrPV-aNCV chimera (MOI 5) at the indicated h.p.i. Shown are representative gels from at least three independent experiments.