Table 2.

Gene-specific primers used in this study.

Name	Sequences (5′–3′)	Function
ohrR MU F	GCTGTGGGTGGATATCAGGA	Construction of ∆ohrR
ohrR MU R	CAGTGACTCGTCTTAAAGAT	Construction of ∆ohrR
pk18_ohrup_F	CGAGCTCGGTACCCGGGCGG GACAACGAATAATTTTG	Construction of ∆ohr
ohrup-ohrdo-R	CCAATGAAGCAAGTGAAGCA TTATTTAGCTATATTTGTACGGAGC	Construction of ∆ohr
ohrup-ohrdo-F	GCTCCGTACAAATATAGCTA AATAATGCTTCACTTGCTTCATTGG	Construction of ∆ohr
ohrdo-pK18-R	AACGACGGCCAGTGCCATTT ACGTCTTGCTGGTCGTG	Construction of ∆ohr
pk18_ ohrRup_F	CGAGCTCGGTACCCGGGCAT CTACCCCTTTTGGCAAT	Construction of ∆ohrR∆ohr
ohrRup- ohrdo-R	CCAATGAAGCAAGTGAAGCA CCTCAGAAAACTAATGGGTGCT	Construction of ∆ohrR∆ohr
ohr Rup- ohr do-F	AGCACCCATTAGTTTTCTGA GGTGCTTCACTTGCTTCATTGG	Construction of ∆ohrR∆ohr
nptII-F	ATGATTGAACAAGATGGATTGC	Amplification of kanamycin resistant gene
nptII-R	TCAGAAGAACTCGTCAAGAAG	Amplification of kanamycin resistant gene
gyrase-PF	AACCTATATTTGCTAGGGAG	Amplification of gyraeB promoter region
gyrase-PR	TACTAGAGGAATCATAAGCC	Amplification of gyraeB promoter region
ohr-1 up	ACCAATTTTTTTACAAAATT GACTTGTTAAAATAATTAAGTCGTG	Amplification of ohr-c promoter region
ohr-1 bo	CACGACTTAATTATTTTAAC AAGTCAATTTTGTAAAAAAATTGGT	Amplification of ohr-c promoter region
ohr-2 up	TACGATATATTTGCATTCAA TTTAATTTAGGAATAAAAAG	Amplification of ohr promoter region
ohr-2 bo	CTTTTTATTCCTAAATTAAA TTGAATGCAAATATATCGTA	Amplification of ohr-c promoter region
Ohr-3_PF	TTGACTTGTTAAAATAATTAAGTCG	Amplification of ohr-c promoter region
ohr_PF	ACCAATTTTTTTACAAAATT	Amplification of ohr-c promoter region
ohr_PR	CTTTTTATTCCTAAATTAAA	Amplification of ohr-C promoter region
pMAC_ohr_PF	GGCCGATATAAGCTCTATTT	Amplification of ohr-p promoter region
pMAC_ohr_PR	TGTATATTACCTTGCTTAAT	Amplification of ohr-p promoter region
ohrR _kpnI_flag-R	GGGGTACCCTTGTCGTCATCGTCTTTG TAGTCTTCAGTCACAATATTAAACG	Construction of pOhrR-C-Pohr-ohr-C
ohrR F	CGGGATCCATGGACCAAGACTGTCAAA A	Specific primer of chromosomal ohrR-C
ohrR R	GGGGTACCTTATTTAGCTATATTTGTAC	Specific primer of chromosomal ohrR-C
ohrR qF	TGGACCAAGACTGTCAAAATC	qRT-PCR primer for ohrR-C
ohrR qR	TCCCACAACACCAACATCAC	qRT-PCR primer for ohrR-C
ohr qF-2	AAGCAACAGGTGGCCGTGAT	qRT-PCR and specific primer of chromosomal ohr-C
ohr qR-2	ACCGACTTCACCTTCAACATACGC	qRT-PCR and specific primer of chromosomal ohr-C
ABpMAC_ohrR_F	TGTCCAAGAATCAGCTTTGCT	qRT-PCR and specific primer of ohrR-p
ABpMAC_ohrR_R	TTTGTCCAAGATCACCCACA	qRT-PCR and specific primer of ohrR-p
ABpMAC_ohr_F	AAAGGTGATGCAACGAATCC	qRT-PCR and specific primer of ohr-p
ABpMAC_ohr_R	GTCAAGGCAAATCCACCATT	qRT-PCR and specific primer of ohr-p
gyrB-F	GGCGGTTTATCTGAGTTTGT	qRT-PCR primer for gyrase gene of A. baumannii
gyrB-R	TTTGTGGAATGTTGTTTGTG	qRT-PCR primer for gyrase gene of A. baumannii