Table 1. Restriction enzyme (RE) accessibility of N-2 and N-3 sites in phosphate starved cells measured in this study and corresponding accessibility values of Brown et al., 2013 (RE accessibility with mean ± standard deviation of two independent biological replicates and the fold-change standard deviation calculated using standard error propagation).

The sticky N-3 mutants feature manipulated DNA sequences at the N-3 site, which decrease the RE accessibility at the N-3 site compared to the wild-type. In our study, this sticky N-3 also decreases the accessibility of the N-2 site. In stage 2, we tested which regulated on-off-slide models with compatible configuration distribution in stage 1 can at the same time reproduce the accessibility fold-changes at sites N-2 and N-3 for both sticky N-3 mutants.

	Wild type	Sticky N-3 mutant 1		Sticky N-3 mutant 2
	accessibility	accessibility	wt fold-change	accessibility	wt fold-change
N-2 RE (ClaI)	${\displaystyle 64.0\pm 1.4\mathrm{\%}}$	$43.5\pm 2.1$	$0.68\pm 0.04$	$47.5\pm 4.9$	$0.74\pm 0.08$
N-2 Brown et al.	82%
N-3 RE (HhaI)	$58.5\pm 2.1$	$30.5\pm 13.4$	$0.52\pm 0.23$	$36.5\pm 7.8$	$0.62\pm 0.13$
N-3 Brown et al.	55%