Figure 5.

Spermidine prevents ethanol and and LPS-collagen deposition. Thick (4–6 µm) sections from mouse livers treated in the absence (control) or presence of ethanol (EtOH), LPS and/or spermidine (SPN) as indicated were stained with Sirius red to monitor collagen deposition (A–D). Labeling was only observed in sections from mice treated with ethanol alone or additionally treated with LPS (B,C). Bar = 100 µm Total hydroxyproline was detected colorimetrically from control or treated mice as an additional indicator of collagen deposition (E). For each experimental condition, four mice were analyzed (n = 4). Values are plotted as µg hydroxyproline detected per mg of liver. The values in E are means of triplicate experiments ± SE.