Table 1.
Phenolic Profile | |||||||||
---|---|---|---|---|---|---|---|---|---|
Peak | Identified Compound | Quantification (mg/g Extract) | |||||||
Basil | Sage | ||||||||
1 a | 4-Hydroxy-7-O-(3′hydroxy-4′-O-glucosylbenzyl)benzyl | 1.00 ± 0.05 | n.d | ||||||
2 b | Luteolin-O-glucuronide | n.d | 12.77 ± 0.07 | ||||||
3 c | Rosmarinic acid | 17.90 ± 0.37 | 23.42 ± 0.26 | ||||||
Total | 18.90 ± 0.41 | 36.19 ± 0.33 | |||||||
Antioxidant activity | |||||||||
Δt 120 min | Δt 180 min | ||||||||
OxHLIA (IC50 values) | Basil | 60 ± 1 | 87 ± 1 | ||||||
Sage | 2.6 ± 0.2 | 8.8 ± 0.4 | |||||||
Trolox | 41 ± 1 | 63 ± 1 | |||||||
Antimicrobial activity | |||||||||
Sage | Basil | E211 | E224 | ||||||
MIC | MBC | MIC | MBC | MIC | MBC | MIC | MBC | ||
Antibacterial | Staphylococcus aureus | 1 | 2 | 2 | 4 | 4 | 4 | 1 | 1 |
Bacillus cereus | 1 | 2 | 1 | 2 | 0.5 | 0.5 | 2 | 4 | |
Listeria monocytogenes | 1 | 2 | 2 | 4 | 1 | 2 | 0.5 | 1 | |
Escherichia coli | 1 | 2 | 2 | 4 | 1 | 2 | 0.5 | 1 | |
Salmonella Typhimurium | 1 | 2 | 2 | 4 | 1 | 2 | 1 | 1 | |
Enterobacter cloacae | 1 | 2 | 2 | 4 | 2 | 4 | 0.5 | 0.5 | |
MIC | MFC | MIC | MFC | MIC | MFC | MIC | MFC | ||
Antifungal | Aspergillus fumigatus | 0.25 | 0.5 | 0.25 | 0.5 | 1 | 2 | 1 | 1 |
Aspergillus niger | 0.5 | 1 | 0.5 | 1 | 1 | 2 | 1 | 1 | |
Aspergillus versicolor | 0.25 | 0.5 | 0.5 | 1 | 2 | 2 | 1 | 1 | |
Penicillium funiculosum | 0.25 | 0.5 | 0.25 | 0.5 | 1 | 2 | 0.5 | 0.5 | |
Penicillium verrucosum | 0.25 | 0.5 | 0.25 | 0.5 | 2 | 4 | 1 | 1 | |
Trichoderma viride | 0.5 | 1 | 0.5 | 1 | 1 | 2 | 0.5 | 0.5 |
n.d—non detected. Calibration curve standards: a cinnamic acid (y = 1E + 06x—222,204, R2 = 0.9993, LOD = 0.12 µg/mL and LOQ = 0.83 µg/mL); b—quercetin-3-O-glucoside (y = 34843x—160173; R2 = 1.000; LOD = 0.21 µg/mL and LOQ = 0.71 µg/mL); c—rosmarinic acid (y = 191291x—652903; R2 = 0.999; LOD = 0.15 µg/mL and LOQ = 0.68 µg/mL).