Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells

Abstract

Cancerous inhibitor of protein phosphatase 2A (CIP2A) is involved in immune response, cancer progression, and Alzheimer's disease. However, an understanding of the mechanistic basis of its function in this wide spectrum of physiological and pathological processes is limited due to its poorly characterized interaction networks. Here we present the first systematic characterization of the CIP2A interactome by affinity-purification mass spectrometry combined with validation by selected reaction monitoring targeted mass spectrometry (SRM-MS) analysis in T helper (Th) 17 (Th17) cells. In addition to the known regulatory subunits of protein phosphatase 2A (PP2A), the catalytic subunits of protein PP2A were found to be interacting with CIP2A. Furthermore, the regulatory (PPP1R18, and PPP1R12A) and catalytic (PPP1CA) subunits of phosphatase PP1 were identified among the top novel CIP2A interactors. Evaluation of the ontologies associated with the proteins in this interactome revealed that they were linked with RNA metabolic processing and splicing, protein traffic, cytoskeleton regulation and ubiquitin-mediated protein degradation processes. Taken together, this network of protein-protein interactions will be important for understanding and further exploring the biological processes and mechanisms regulated by CIP2A both in physiological and pathological conditions.

Graphical abstract

A schematic representation of the CIP2A interactome study: CIP2A immunoprecipitation (IP) was performed after 72 ​h of polarization of naïve T cells towards the Th17 lineage. Two CIP2A specific antibodies (Ab1 and Ab2), targeting the N-terminal structured region and unstructured C-terminus, were used together with their respective IgG (IgG1 and IgG2) control antibodies. Following LC-MS/MS analysis to identify the CIP2A associated proteins, statistical analysis using the Significance Analysis of INTeractome (SAINT) algorithm was used to distinguish non-specific interactions.

Highlights

• •The first characterisation of the CIP2A interactome in Th17 ​cells.
• •Key interactions validated by targeted SRM-MS proteomics, western blot and confocal microscopy.
• •Pathway analysis of the interactome revealed interrelationships with proteins across a broad range of cellular processes.
• •The study identifies for the first time the interaction of phosphatase PP1 with CIP2A.

Introduction

Protein Phosphatase 2A (PP2A) is a recognized tumor suppressor that imparts crucial functions in regulating cell proliferation, differentiation, and apoptosis (Apostolidis et al., 2016, Apostolidis et al., 2013, Eitelhuber et al., 2011, Janssens and Goris, 2001, Katsiari et al., 2005, Long et al., 2014, Wlodarchak and Xing, 2016). Accordingly, down regulation of PP2A has been observed as a feature in many cancers. Cancerous Inhibitor of PP2A (CIP2A) was first characterized as an endogenous inhibitor of PP2A in cancer cells (Junttila et al., 2007). Previous studies have shown that many of the known actions of CIP2A are mediated through inhibition of PP2A (Laine et al., 2013, Niemelä et al., 2012). Overexpression of CIP2A has been associated with a poor prognosis in several human malignancies, and CIP2A has thus been seen as a potential therapeutic target for cancer therapy (Junttila et al., 2007, Khanna et al., 2013, Lucas et al., 2016). CIP2A supports the activity of many critical onco-proteins (Akt, MYC, E2F1) and promotes malignancy in most cancer types via PP2A inhibition (Junttila et al., 2007). Structurally, CIP2A forms a homodimer and this dimerization promotes its interaction with the PP2A regulatory subunits B56α and B56γ (Wang et al., 2017). Interestingly, inhibition of either CIP2A dimerization or B56α/γ expression destabilizes CIP2A in cancer cells (Wang et al., 2017). In Alzheimer's disease (AD), enhanced expression of CIP2A in the brain results in PP2A inhibition and tau protein hyperphosphorylation, which leads to abnormal tau localization with memory deficits and other pathological conditions (Shentu et al., 2018).

T helper (Th) 17 (Th17) cells are found at mucosal surfaces where they regulate tissue homeostasis (Guglani and Khader, 2010, Kleinewietfeld et al., 2013, Kleinewietfeld and Hafler, 2013, Romani, 2011). Dysregulation of Th17 ​cell differentiation, however, has been associated with a number of autoimmune inflammatory diseases (Bedoya et al., 2013, Di Cesare et al., 2009). Following our earlier report on the involvement of CIP2A in T cell activation (Côme et al., 2016), we subsequently observed that CIP2A negatively regulates Th17 ​cell differentiation, whereby CIP2A depletion resulted in enhanced IL17 expression both in human and mouse Th17 ​cells (Khan et al., 2020). Thus, CIP2A could have important implications in the differentiation and regulation of Th17 ​cells. Although CIP2A has been shown to regulate a range of pathological and physiological conditions, there has not been any systematic analysis of the full range of signaling pathways and processes regulated by CIP2A.

In the current study, we used immunoprecipitation (IP) followed by label free quantitative proteomics to gain insight into the proteins that are associated with CIP2A. We present the first interactome of CIP2A, with validation of interactors by selected reaction monitoring targeted mass spectrometry (SRM-MS) as well as by immunoprecipitation followed by western blotting (IP-WB) and Proximity ligation assay (PLA) followed by confocal microscopy. In addition to its known interaction with the regulatory subunit A and B of phosphatase PP2A, our data demonstrated CIP2A interaction with the PP2A catalytic subunits namely PPP2CA and PPP2CB. Furthermore, both the regulatory (PPP1R18, and PPP1R12A) and catalytic (PPP1CA) subunits of phosphatase PP1 were identified among the top interactors of CIP2A. This data further implicates the involvement of CIP2A in a number of biological processes that has previously not been described, and provides a basis for comprehensive understanding of CIP2A for translational studies targeting CIP2A in human diseases.

Results

Mass spectrometry analysis identify novel CIP2A binding partners

To identify the proteins interacting with CIP2A and thereby facilitate the understanding of its functions in human Th17 ​cells, IP was performed after 72 ​h of polarization of CD4+ T cells towards the Th17 lineage (Fig. 1A; S1 A-C). The 72 ​h time point was selected since at this stage of polarization IL-17 could reliably be detected in the supernatant. The cell model for the study was rationalized by our recent findings indicating that CIP2A is involved in T-cell activation (Côme et al., 2016) and important for Th17 ​cell differentiation (Khan et al., 2020). Two CIP2A specific antibodies (Ab1 and Ab2), recognizing distinct regions of CIP2A, together with their respective control IgG antibodies (IgG1 and IgG2), were used in two biological replicates. Ab1 was a monoclonal antibody targeting the N-terminal structured region of the CIP2A protein (residues surrounding Val 342) (Wang et al., 2017), while Ab2 was of polyclonal origin targeting the C-terminus (Hoo et al., 2002) (Fig. 1B).

Fig. 1.

CIP2A interactome top protein interaction in Th17 ​cells. (A) Western Blot (WB) analysis of CIP2A immunoprecipitated (IP) with two antibodies (Ab1 and Ab2), specific to different regions of CIP2A, in 72h polarised Th17 ​cells. For each CIP2A targeting antibody, an IgG control antibody was used. The figure is a representative WB of two experiments. Input, IgG control IP and CIP2A IP reactions are shown. (B) Schematic diagram of the human CIP2A protein structure. Regions specific to the antibodies used in the pulldown are shown. Ab1: against residues surrounding valine 343 (monoclonal), Ab2: 618-905 peptide (polyclonal). Protein domain borders are based on a previous publication (Wang et al., 2017) in which the Armadillo repeat, and the dimerization interface are shown in the CIP2A structured domain studied by Wang et al., 2017. (C) A Venn diagram showing the number of common and unique CIP2A interacting proteins identified with two distinct antibodies targeting CIP2A based on the mass spectrometry analysis. For details, see the method section. (D) Heatmap representation of the top fifty CIP2A interactors identified with both antibodies. R1 and R2 represent data from two biological replicates. Normalized spectral intensities are plotted as a heatmap to display protein abundance in respective IP reaction.

The availability of highly specific antibodies against CIP2A facilitated the IP of endogenous CIP2A without the requirement for overexpression. After preparation for proteomic analysis, the digested samples were analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) to identify the CIP2A associated proteins. Analysis of the data revealed 680 proteins meeting the criteria of identification of two or more unique peptides with a confidence threshold of 99%. In order to distinguish non-specific interactions and common contaminants, statistical analysis of the label-free quantification (LFQ) intensity data was made using the Significance Analysis of INTeractome (SAINT) algorithm (Choi et al., 2011). Using a threshold for the calculated SAINT probability scores of greater than or equal to 0.95, and exclusion of proteins represented in the contaminant's repository (Mellacheruvu et al., 2013) with a frequency of >60%, the list of candidate common interacting proteins was reduced to 335 (Supplementary Tables 1A–C). Over 80% interactors were common to both antibodies specific to CIP2A (Fig. 1C; S1D; Supplementary Table 1A-C). Intriguingly, the CIP2A interactors with both antibodies contained its known interacting partners PP2A-A (PPP2R1A) structural subunit and PP2A B regulatory subunit (Supplementary Tables 1A–C). Comparison of the exclusive CIP2A interacting proteins detected with either antibody revealed that several proteins associated with mRNA processing were detected only with Ab1, whilst the proteins particularly associated with Ab2 mostly included those involved in complement and coagulation pathways. To exemplify the interactions between CIP2A and its interaction partners, the top fifty common proteins, selected based on the detected signal intensity with both antibodies, were plotted in the form of a heatmap (Fig. 1D).

Distribution of CIP2A interactome between the nucleus and cytoplasm

Analysis of the cellular location of CIP2A in epithelial cells has indicated that it is mainly expressed in the cytoplasm (Junttila et al., 2007, Hoo et al., 2002). Further data indicate some representation in the nuclear fraction of epithelial cancer cells (Myant et al., 2015). To evaluate the distribution of CIP2A in Th17 ​cells, confocal microscopy measurements were conducted in this study. For these measurements, 4,6-diamidino-2-phenylindole (DAPI), and Phalloidin were used to stain the nuclear and cytoplasmic fractions, respectively (Fig. 2A). Statistical analysis of the confocal microscopy images revealed CIP2A is predominantly located in the cytoplasmic compartment of Th17 ​cells, in addition to some nuclear expression (Fig. 2B). Confirmatory measurements were provided with WB analysis of the cytoplasmic and nuclear fractionations of 72h polarised Th17 ​cells in two biological replicates, where vimentin and tubulin were used as control proteins for the nuclear and cytoplasmic fractions, respectively (Fig 2C). Whilst the observed expression of CIP2A was greater in the cytoplasmic region, CIP2A was also detected in the nuclear region of 72h differentiated Th17 ​cells (Fig 2C).

Fig. 2.

Cellular distribution of CIP2A and the proportions of CIP2A interactome in Th17 ​cells. (A) Immunostaining for the study of CIP2A localisation in 72h polarised Th17 ​cells. A representative image of three replicates are shown. Cells were stained for endogenous CIP2A (green). Phalloidin (red) and DAPI (blue) were used to stain cytoplasmic and nuclear regions, respectively. Scale bar is 7μm. (B) Statistical analyses of CIP2A expression for cellular localization studies from three replicates using GraphPad Prism version 8.0 ​d for Mac OS X (GraphPad Software). Statistical analysis was made by a two-tailed paired Student's T-test, where p-value ∗∗ represents <0.01. The error bars represent standard error of the mean. (C) WB analysis of two replicates for the cytoplasmic and nuclear fractions for CIP2A sub-cellular localisation in 72h polarised Th17 ​cells. WB probes with antibodies as controls for nuclear and cytoplasmic fractions detecting vimentin and tubulin proteins, respectively. WCL, NF and CF represent Whole Cell lysate, Nuclear Fraction, and Cytoplasmic Fraction, respectively. (D) Enriched cellular locations of the proteins in the CIP2A interactome presented in the form of pie chart. The enrichment analysis was performed using the Ingenuity pathway analysis (IPA; Qiagen).

Ingenuity Pathways Analysis (IPA; Qiagen) was used to map cellular locations of the proteins interacting with CIP2A. The analysis revealed that the cytoplasm (42%) and nucleus (43%) constituted the most frequent cellular locations of the associated proteins, whilst the plasma membrane (8%), extracellular space (4%), and undefined (other, 3%) accounted for the remainder (Fig. 2D). The observed distribution of the proteins detected in the CIP2A interactome is consistent with its association in these compartments in Th17 ​cells.

Cellular processes regulated by CIP2A interactome

To gain an overview of the biological processes associated with CIP2A, we analyzed the CIP2A interactome data with several Gene Ontology and interaction bioinformatics tools. To collate the known interactions between the proteins, a network was constructed using the STRING database (Szklarczyk et al., 2017). The resulting network was visualized with Cytoscape (Shannon et al., 2003), revealing “RNA metabolism or splicing” as the process most frequently linked with the associated proteins (Fig. 3A; Supplementary Tables 2 and 3). The top 20 proteins associated with RNA metabolic process, RNA splicing via transesterification and RNA splicing via spliceosome are represented in the form of heatmaps (Fig. S3A–C). In agreement with these results, a recent phosphoproteome screen also identified RNA splicing as one of the most significantly CIP2A-regulated processes (Kauko et al., 2018).

Fig. 3.

The CIP2A protein interaction network. (A) The interaction network among the CIP2A interacting proteins. Protein-protein interactions for the network were downloaded from the STRING database and visualized using the Cytoscape software. Nodes in the network were clustered using the Markov clustering algorithm. The identified clusters with more than two members are indicated by distinct colors. A representative GO biological process term is shown for each cluster with four or more members. The GO enrichment analysis was performed using DAVID against a Th17 proteome reference background (Tripathi et al., 2019). The median MS intensity differences between the CIP2A immuno-precipitates and the IgG controls are shown as node inner color from white to grey according to increasingly stronger expression in the CIP2A immuno-precipitates compared to the IgG controls. (B) Enriched Biological processes associated with the proteins in the CIP2A interactome in human Th17 ​cells. The enrichment analyses was performed using PANTHER with the enrichment calculated from a background of proteins detected in Th17 proteomic reference background (Tripathi et al., 2019). The scale is –log FDR. (C) Functional classification of the CIP2A interactome in Th17 ​cells. The proportions of different enriched functional classes in the CIP2A interacting proteins. The enrichment analysis was performed using IPA (Qiagen).

To provide additional context and an appropriate milieu, functional enrichment analysis was made relative to a combined list of the proteins detected together with those found in a recent cellular proteomics analysis of human Th17 ​cells (Tripathi et al., 2019) rather than relative to a background of the whole genome. For this comparison the bioinformatics tools DAVID (Huang et al., 2009a, Huang et al., 2009b) and PANTHER (Mi et al., 2017, Mi et al., 2013) were used. The enriched Th17 pathways are represented in Fig. 3B. Again, mRNA processing and splicing were found to be the most significant biological process linked with CIP2A interactors. Collectively, these results suggest the involvement of CIP2A in multiple processes.

IPA analysis was also used to summarize the molecular functions of the CIP2A interacting proteins (Fig. 3C). This revealed enzymes (∼24%) as the major functional class of the proteins. In addition, transcription regulators (∼7.4%) and transporters (6.1%) are the next major class of proteins. A small fraction of proteins was classified as phosphatases (3.4%), kinases (2.0%), translation regulators and ion channels (1.0% each), cytokines and peptidases (<1% each). For a major fraction of the proteins in CIP2A the interactome (54%), however, analysis with the IPA database indicated that no specific function could be assigned (marked as “others” in Fig. 3C). Overall the IPA analysis indicated that CIP2A interacts with proteins with a variety of different functions. It is possible that via its interaction with these proteins CIP2A regulates a range of processes in different cell types, some of which are as of yet undefined.

Thus, the functional and network analysis of the associated proteins revealed how CIP2A and its interacting partners participate in a broad set of essential cellular processes, ranging from RNA metabolic processes and splicing to viral processes.

Validation of the Th17 ​cell CIP2A protein interactions

Validation experiments were performed to verify the interaction of selected proteins with CIP2A. The choice of targets focused on those with most significant interactions and nodal positions in CIP2A interactome (Fig. 1D). The validation experiments were carried out by SRM-MS, PLA confocal microscopy and/or IP-WB. analysis. The choice of method depended on the availability of suitable working antibodies or proteotypic peptide signatures, for PLA confocal microscopy and IP-WB or SRM-MS analysis, respectively.

SRM-MS analysis provided successful validation of twenty CIP2A interaction partners using immunoprecipitates from three independent biological replicates (Fig 4A; Supplementary Tables 4–5). We confirmed the known (Junttila et al., 2007) interaction of CIP2A with PP2A regulatory subunit A (PPP2R1A) (Fig. 4). In addition, we identified and validated among the CIP2A interactors, PP2A catalytic subunits PPP2CA and PPP2CB (Fig. 4A; S4A and Supplementary Table 5).

Fig. 4.

CIP2A interactome validation by SRM mass spectrometry. Selected reaction monitoring targeted mass spectrometry (SRM-MS) validation of the CIP2A interactome. CIP2A IP was performed using Ab2 antibody in 72h polarised Th17 ​cells and twenty proteins were validated by targeted SRM-MS. Averaged results from three replicates are presented in the form of a box plot. Statistical analysis was made by a two-tailed paired Student's T-test, where ∗ p-value represents <0.05; and ∗∗∗ p-value represents <0.001. The error bars represent 95% confidence interval.

Interestingly, we identified PP1 regulatory (PPP1R18 and PPP1R12A) and catalytic (PPP1CA) subunits as the top CIP2A interacting proteins (Fig 1D). By SRM-MS analysis, we further confirmed CIP2A interaction with the catalytic subunits (PPP1CA and PPP1CB) of phosphatase PP1 (Fig. 4A; S4A and Supplementary Table 5). Further, in a recent phospho-proteome analysis of CIP2A-regulated proteins, LMNB1 was found to be dephosphorylated in CIP2A silenced cells (Kauko et al., 2018) and here its interaction was detected and subsequently validated in the CIP2A interactome by SRM-MS analysis (Fig. 4). Previous studies have revealed the interaction of with phosphatase PP2A regulatory A subunit (Junttila et al., 2007). Accordingly, we observed and validated this interaction with PP2A-A in Th17 ​cells (Fig 5A). Additionally, we validated the interaction with the PP2A catalytic subunit PPP2CA. Notably, we were also able to validate interaction between CIP2A and PP1 catalytic subunit by co-immunoprecipitation followed by WB (Fig. 5B). The interaction of CIP2A with both PPP2CA, and PPP1CA could be because of high structural similarity of the two proteins (Shi, 2009). Further, PPP1CA interaction was independently validated by Proximity ligation assay (PLA) (Fig. 5C). The negative control PLA, conducted from the same samples without primary antibodies, did not show any background signals (Fig. 5C).

Fig. 5.

Validation of CIP2A protein interaction by IP-Western Blot and PLA Confocal microscopy. (A) CIP2A IP performed in Th17 ​cells and WB detection to validate the CIP2A known interaction with phosphatase PP2A-A. Total lysate (Input) and control IP (IgG) and CIP2A IP using Ab2 are shown on the blot. A conformational specific secondary antibody was used to probe proteins without interference from the denatured IgG heavy (50 kDa) and light chains (25 kDa). (B) WB validation of the CIP2A-PPP1CA interaction. CIP2A immunoprecipitated with two different antibodies (Ab1 and Ab2) in Th17 ​cells (72h) and detected as in A. (C-D) Proximity ligation assay (PLA) assay validation of the CIP2A interaction with PPP1CA (C) and TRIM21 and UBR5 (D) in Th17 ​cells (D). The GFP antibody was used as a negative control in the PLA. DAPI was used to stain the nuclei. Scale bar is 7μm. Representation of the interaction between CIP2A-PPP1CA (C) or TRIM21 and UBR5 with CIP2A (D) by PLA as a univariate dot plot. The average number of PLA signals (spots) per cell (n ​= ​10 images from a representative of three experiments) was plotted. Statistics by paired two tailed T-test, where ∗ represents P ​< ​0.05. and ∗∗< 0.01. Scale bar is 7μm. (E) Western blot validations representative of interactions between TRIM21, IRF4, STAT1 and PP2A with CIP2A in Th17 ​cells at 72h. IP was performed using Ab1 and Ab2 CIP2A antibodies and a conformational specific secondary antibody was used to detect the protein as in Fig. 5A. A representative Western blot of four biological replicates is shown, the remaining three Western blot replicates are shown in Figs. S5B–D. (F) CIP2A inhibition leads to enhanced Nf-κB activation. CIP2A is silenced in three different individual donor cell pools, and CIP2A, phospho (pp52 and pp110) and total (p52 and pp110) Nf-κB2 and Actin are shown on the blot. The bar chart on the right shows the quantification of the phosphorylation levels of p110 and p52 in CIP2A silenced Th17 ​cells. Quantification was performed using ImageJ software in which phosphorylation values were normalised with respective total protein p110 and p52 expression. Statistical significance calculated using Student's t-test (two-tailed paired) where ∗ denotes p-value <0.05.

TRIM21 was amongst the most enriched proteins in this interactome (Fig. 1D), and the interaction was validated by both SRM-MS (Fig. 4A) and PLA confocal microscopy (Fig. 5D). Interaction with the ubiquitin ligase UBR5 was validated by PLA confocal microscopy, both in Th17 ​cells and HeLa cells (Fig. 5D and S5A). In addition, IP-WB analysis were made for STAT1, IRF4 and TRIM21, which were consistent with the targeted SRM-MS analysis (Fig. 4, Fig. 5E; Fig S5B-DC). Further, NFKB2 was also confirmed to be a CIP2A interacting protein (Fig. 4A, S4). Importantly, we found that CIP2A inhibition led to increased NF-KB2 activation (Fig. 5F). In CIP2A silenced Th17 ​cells, enhanced phosphorylation of P110 and P52 was detected by WB analysis (Fig. 5F). The direct interaction of CIP2A with NF-KB2 and its role in NF-KB mediated gene regulation remains to be further studied.

Discussion

With these analyses of the CIP2A interactome in the Th17 milieu, and in relation to phosphatase biology, we verified the well-known interaction with structural subunit PP2A-A (PPP2R1A) and PP2A regulatory B subunit (Supplementary Tables 1A–C). Previously, Wang et al. (2017) demonstrated direct interaction between CIP2A and PP2A regulatory B subunits B56α and B56γ by yeast two-hybrid analysis (Y2H), and Junttila et al. (2007) discovered PP2A-A subunit interaction with CIP2A. In the present report, we detected and validated for the first time the interaction of CIP2A with the PP2A catalytic subunit (PPP2CA and PPP2CB). All of these interactions were validated with SRM-MS analysis (Fig. 4; and Supplementary Tables 4–5). In an earlier study, we observed that CIP2A is induced already after 24h of T cell activation (Côme et al., 2016). In view of the dynamics of Th17 differentiation and the contrast in protein expression at 24 and 72h (Tripathi et al., 2019, Tuomela et al., 2016) there could be changes in the interaction partners during differentiation.

Previously, in studies with T regulatory (Treg) cells, PP2A enhanced catalytic activity was identified as an indispensable element of the suppressive function of Treg cells (Apostolidis et al., 2016). Treg cells maintain tolerance and prevent autoimmune diseases by suppressing effector cells and modulating the immune system. Positive correlations with PP2A expression and the severity of systemic lupus erythematosus (SLE) and overproduction of IL17A cytokine have been previously reported (Katsiari et al., 2005, Sharabi et al., 2018). Further, in a recent study using PP2A catalytic subunit deficient mice and its chemical inhibitors, an essential role of PP2A through the regulation of the SMAD signaling in Th17 ​cells was reported (Sharabi et al., 2018, Xu et al., 2019) It remains to be studied if the interaction between CIP2A and the catalytic subunit of PP2A regulates the suppressive function of Treg cells and the pro-inflammatory Th17 ​cells or SLE.

The regulatory (PPP1R18 and PPP1R12A) and catalytic (PPP1CA) subunits of phosphatase PP1 were among the top CIP2A interacting partners. Interaction between CIP2A and PP1 has not been previously shown and further studies are required to demonstrate the importance of this association. Notably, in this study, the catalytic subunits of both phosphatases PP2A and PP1 were detected and confirmed in the CIP2A interactome. This interaction could arise due to the structural similarity between catalytic subunits of the PP2A and PP1 phosphatases (Shi, 2009). In a different milieu, CIP2A might similarly engage the catalytic subunits of other serine/threonine phosphatases, which could be an interesting focus for future studies.

In rheumatoid arthritis (RA) inflamed synovium, increased expression and activity of phosphatase PP1 results in reduced transcriptional activity of FOXP3 through specific dephosphorylation of the Ser418 site (Nie et al., 2013). Intriguingly, CIP2A expression in RA has been associated with the histopathological score of synovitis, as well as apoptotic resistance and invasive function of fibroblast-like synoviocytes (Lee et al., 2012, Lee et al., 2013). Thus, interaction between CIP2A and PP1 may play a role in regulatory T cell function and inflammation in RA.

Our study revealed that RNA processing and splicing was the most frequent process associated with the CIP2A interacting proteins. Previously, it was reported that alternative splicing of FOXP3 modulates Th17 differentiation (Mailer et al., 2015). In cancer cells, splicing results in the production of protein variants that promote anti-apoptotic pathways by opposing function of the same gene that support apoptotic cell death pathways in normal cells (David and Manley, 2010, Schwerk and Schulze-Osthoff, 2005). Further studies are needed to determine the role of CIP2A and its associated interactome in the regulation of RNA processing and splicing in Th cell differentiation and cancer cell development.

Several kinases related to T cell receptor (TCR) signaling (i.e. CSK and LCK) were identified as CIP2A interactors (Chow et al., 1993, Veillette et al., 2002). The initiation of TCR signalling is regulated by the SRC-related protein tyrosine kinase family, which includes LCK, whereas CSK is a potent inhibitor of TCR signalling (Chow et al., 1993). Notably, we have observed reduced T cell activation in CIP2A depleted T cells (Côme et al., 2016). It will be interesting to study if the loss of CIP2A binding to LCK or CSK contributes to impaired T cell activation.

The adaptor protein DOCK8 was amongst the most highly enriched proteins detected in this interactome. Combined immunodeficiency in humans is associated with loss-of-function mutations in the gene encoding DOCK8 (Zhang et al., 2009). Notably, the phenotype in immunodeficiency due to mutations in the gene encoding the transcription factor STAT3 is very similar to that caused by the loss of DOCK8 (Su, 2010). Jabara et al. reported that in B cells DOCK8 and STAT3 are components of the same signalling cascade (Jabara et al., 2012). In Th17 ​cells, CIP2A interacts with both DOCK8 and Lyn, and regulates STAT3 phosphorylation (Khan et al., 2020). It could be revealing to study whether CIP2A regulation of STAT3 phosphorylation is dependent on its interaction with Lyn and DOCK8.

Several of the proteins of this interactome, including IFI16, IRF4, MX2, STAT1 and TRIM21, have functions related to viral response and interferon signalling. Notably amongst these, the increased activity and DNA binding of IRF4 has previously been found in association with the increased capacity of PP2Ac transgenic T cells to produce IL-17 in response to TCR stimulation (Apostolidis et al., 2013). CIP2A directly interacts with and inhibits PP2A in cancer cells and depletion of CIP2A in T cells results in enhanced IL17 (Khan et al., 2020). On the basis of these associations, the influence of CIP2A interaction on the DNA binding of IRF4 in T cells could be an important focus of future studies.

Amongst the CIP2A interacting transcription factors were RUNX1 and STAT1, which were validated by IP-WB and SRM-MS. Future studies could address the relationship between CIP2A and STAT1 in Th17 ​cells. RUNX1 is important for the development of Th17 ​cells as it directly binds the promoter of RORC, a transcription factor important for Th17 ​cell development, and mediates its transactivation (Lazarevic et al., 2010). In addition, Runx1 has been reported to bind to the IFNγ locus and induces its expression in Th17 ​cells resulting in development of pathogenic IL17⁺ IFNγ⁺ Th17 Cells (Lazarevic et al., 2010, Wang et al., 2014). In view of the latter and our data of CIP2A-RUNX1 interaction, it will be of interest to find out if this interaction plays a role in RUNX1 mediated RORC and IFNγ induction in Th17 ​cells.

TRIM21, an E3 ubiquitin ligase, was prominent in the CIP2A interactome (Fig. 4). TRIM21 has been associated with the pathogenesis of several autoimmune diseases, including SLE and inflammatory bowel diseases (IBDs). TRIM21 plays a protective role in IBD pathogenesis, possibly through inhibition of Th1 and Th17 ​cell immune responses in the intestinal mucosa (Espinosa et al., 2009, Yoshimi et al., 2012, Zhou et al., 2017). Thus, in view of the the detected interaction, it is possible that pathways involving CIP2A are also active in the pathogenesis of autoimmune diseases like SLE and IBD.

Beta-spectrin (SPTBN1) was amongst the top most proteins enriched in CIP2A interactome (Fig. 1D). Beta spectrin is a molecular scaffold protein that is involved in cell shape and the organisation of organelles in the cell (Machnicka et al., 2012). Beta spectrin has been identified as an integral component of beta-amyloid plaques in Alzheimer's disease (AD) and spectrin breakdown products (SBDPs) have been implicated as potential biomarkers for neurodegenerative diseases including AD (Sihag and Cataldo, 1996, Yan et al., 2012). In a recent study of AD, CIP2A was found to be overexpressed and associated with tau hyperphosphorylation, mis-localization and AD-related cellular pathology (Shentu et al., 2018). In mice, overexpression of CIP2A in the brain induced AD-like cognitive deficits (Shentu et al., 2018). Future studies may determine whether CIP2A interaction with SPTBN1 modulates protein aggregation in AD pathology.

In summary, the results of this study present the first characterisation of CIP2A interactome in Th17 ​cells. In addition to the known interactions with the PP2A protein A and B regulatory subunits, our results detail several novel CIP2A interacting proteins. These include CIP2A interaction with the catalytic subunit of PP2A, as well as regulatory and catalytic subunits of PP1. Gene ontology and pathway analysis of the associated proteins suggest CIP2A is involved in several cellular functions that have not previously been described. Taken together, we anticipate that the new information of proteins depicted in this CIP2A interactome and their associated networks, could be useful in understanding the role of CIP2A in regulating cell signalling and its role in various human disorders.

Methods

Human CD4+ T cell isolation, activation, and differentiation

Umbilical cord blood was layered on Ficol (GE Healthcare, Cat# 17-1440-03) for isolation of white blood cells. CD4+ T cells were then isolated using the Dynal bead CD4+ isolation kit from Invitrogen (Cat# 11331D). For activation of T cells, a combination of plate-bound anti-CD3 (Beckman Coulter, REF# IM-1304) and soluble anti-CD28 (Beckman Coulter, REF# IM1376) antibodies was used. For Th17 cultures, cells were activated in presence of cytokines IL-6 (20ng/ml, Roche, Cat# 11138600 001); IL-1β (10ng/ml, R&D Systems, Cat# 201 LB) and TGF-β1 (10ng/ml, R&D Systems, Cat# 240); and neutralizing antibodies anti-IL-4 (1 ug/ml) R&D Systems, (Cat# MAB204) and anti–IFN–γ (1 μg/ml R&D Systems, Cat# MAB-285). As control (Th0), cells were, in parallel, activated in the presence of anti-IL-4 and anti–IFN–γ but without the cytokines.

Flow cytometry

CCR6 surface staining was performed for Th17 ​cell culture polarization control. Cells were stained with the CCR6 antibody (Cat# 559562, eBioscience) in FACS I buffer (1%FBS in PBS) at +4 °C for 30 ​min in dark. Cells were then washed two times with FACS I, and finally re-suspended in FACS I Buffer or 1% Formalin before acquisition in BD LSR II/Caliber (BD Biosciences). Data analysis was performed using FlowJo (BD) or Flowing Software (Version 2.5.1).

Luminex assay

As a Th17 polarization control, IL17 cytokine protein secretion was measured in culture supernatants from 72 ​h Th17 and Th0 cells using Luminex assay (Milliplex MAP human cytokine, Luminex 200 by Luminex xMAP technology), according to the manufacturer's instructions. To avoid cell proliferation bias, the cytokine concentrations were normalized by the number of cells, counted by flow cytometry, from each respective culture.

Immunoprecipitation

CIP2A immunoprecipitation (IP) was carried out with two specific anti-CIP2A antibodies that recognize distinct regions of CIP2A protein and the respective Immunoglobulin G (IgG) were used in control IPs. The Pierce™ MS-Compatible Magnetic IP Kit (Cat# 90409) was used to perform the IP. Briefly, cells were first harvested on ice and the culture medium removed. This was followed by washing the cells twice with PBS. Cells were then re-suspended on ice-cold IP-MS Cell Lysis Buffer, as recommended by the manufacturer, and incubated on ice for 10 ​min with periodic mixing. The lysate was centrifuged at ∼13,000 ​× ​g for 10 minto pellet the cell debris. The supernatant was transferred to a new tube for protein concentration determination using a NanoDrop™ spectrophotometer (Thermo Scientific). Cell lysates (500μg) were incubated with the IP antibody (1:50 dilution). The antibody/lysate solution was diluted with the manufacturer's proprietary IP-MS cell lysis buffer and incubated with mixing overnight at +4 ^oC to form the immune complex. On the next day, the immune complex was incubated with Protein A/G magnetic beads for 1 ​h at room temperature (RT). The beads were then washed and the target antigen eluted with the elution buffer provided and transferred to a new low protein-binding 1.5mL microcentrifuge tube. A vacuum centrifuge was used to dry the eluate prior to preparation for MS or SDS-PAGE and Western blot.

Western blotting

T cells were first resuspended in Triton-X-100 lysis buffer (TXLB). The composition of TXLB used was 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.5% Triton-X-100, 5% Glycerol and 1% SDS. For cell lysis, cells in TXLB were sonicated on ice for 5 ​min using a Bioruptor® sonicator (Diagenode). After cell lysis, the lysate was centrifuged at high speed (16,000 g) and the supernatant was transferred to a new tube to remove cell debris. Protein concentration was estimated using a DC Protein assay (Bio-Rad, Cat# 500-0111). Equal amounts of protein were loaded onto acrylamide gel (Bio-Rad Mini or Midi PROTEAN® TGX precast gels). For the transfer of proteins to the PVDF membrane, mini or a midi transfer packs from Bio-Rad were used depending on the gel size. Primary/secondary antibody incubations were performed in 5% non-fat milk or Bovine serum albumin (BSA) for phosphoproteins in TBST buffer (0.1%Tween 20 in Tris-buffered saline). Quantification of the bands detected was performed using Image J software after normalization relative to the loading control intensities. The following antibodies were used for western blotting, CIP2A antibody 1 (Ab1) (Cell Signaling Technology, Cat# 14805) CIP2A antibody 2 (Ab2) provided by Prof. Jukka Westermarck (Junttila et al., 2007, Hoo et al., 2002); PP2A-A/B (Santa Cruz, Cat# sc-15355); Trim21 antibody (Santa Cruz, Cat # SC25351); Irf4 antibody (Santa Cruz, Cat # SC6059); Beta-actin antibody (Sigma, Cat# A5441). PPP1A/PPP1CA antibody (Abcam, Cat# ab137512), PP1α Antibody (G-4): (Santa Cruz, Cat# sc-271762).

Immunofluorescence

Th17 ​cells were plated onto acid-washed glass coverslips. The cells were then fixed at RT using 4 % PFA for 10 ​min. After fixing, the cells were permeabilized in PBS containing 30% horse serum and 0.1% Triton X-100 for 10 ​min at RT, washed in PBS and then blocked in PBS-30% horse serum for 1 ​h at RT. The cells were then incubated with the CIP2A primary antibody overnight at 4 °C. On the next day, the cells were washed three times using PBS and incubated for 1 ​h at RT with AlexaFluor-conjugated secondary antibodies (Life Technologies). Cells were then mounted with Mowiol® for 30 ​min at 37 °C and Images taken with a Carl Zeiss LSM780 laser scanning confocal microscope equipped with 100 ​× ​1.40 oil plan-Apochromat objective (Zeiss). Corrected total cell fluorescence was calculated from confocal images to quantify the intensities in Th0 and Th17 ​cells (Burgess et al., 2010, McCloy et al., 2014).

Immunofluorescence staining of UBR5 in HeLa cells was performed similarly, the cells were cultured on chambered coverslip (80826, Ibidi). 4% paraformaldehyde was used to fix the cells for 15 ​min at RT, followed by cell permeabilization with 0.5% Triton X-100 in PBS on ice for 5 ​min. Next, the cells were blocked with 10% normal goat serum (Abcam, Cat# ab7481) diluted in PBS for 30 ​min, and followed by incubating with the primary antibodies anti-UBR5 (ab70311, Abcam) overnight at 4 °C. Subsequently, cells were washed with PBS and incubated with Alexa Fluor 488 goat anti-rabbit IgG secondary antibody (Invitrogen, Cat# A-11008) for 1 ​h at RT. After secondary antibody incubation, cells were washed with PBS and nuclei were stained with DAPI (Invitrogen, Cat# D1306) in PBS at RT for 10 ​min. Images were acquired with confocal microscope (LSM780, Carl Zeiss).

Proximity ligation assay

The PLA assay was performed according to the manufacturer's protocol (Duolink® PLA, Sigma). Briefly, Th17 or HeLa cells were plated on coverslips. The cells were fixed with 4% paraformaldehyde for 15 ​min at RT, and then permeabilized with 0.5% Triton X-100 in PBS on ice for 5 ​min. Next, cells were blocked with blocking solution, and incubated in a pre-heated humidity chamber for 30 ​min at 37 °C, followed by incubating with the primary antibodies (in blocking solution) anti-UBR5 (Abcam, Cat# ab70311) and anti-CIP2A (Santa Cruz, Cat# sc-80659), anti-PPP1CA (Abcam, Cat# ab137512), anti-UBR5 (Abcam, Cat# ab70311), anti-TRIM21 (Santa Cruz, Cat# sc-25351), anti-GFP (mouse) (Abcam, Cat# ab1218) and anti-GFP (rabbit) (Invitrogen Cat# A11122) overnight at 4 °C. Subsequently, cells were washed with the kit buffer A, and the PLA probe was incubated in a pre-heated humidity chamber for 1 ​h at 37 °C, followed by ligase reaction in a pre-heated humidity chamber for 30 ​min at 37 °C. Next, amplification polymerase solution for PLA was added, followed by incubating the cells in a pre-heated humidity chamber for 100 ​min at 37 °C. After amplification, the coverslips were washed with the kit buffer B, and mounted with DAPI. The PLA signal was detected by using a confocal microscope (LSM780, Carl Zeiss) and 3i CSU-W1 spinning disk microscope equipped with 100 ​× ​1.4 O Plan-apochromat objective (Zeiss). PLA signals per cell were calculated by dividing the amount of PLA signal dots in one field of view, determined using Cell Profiler software (Carpenter et al., 2006).

Cell transfection with siRNA

CD4+ T cells were isolated from the cord blood and four million cells were transfected in 100 μl volume of Opti-MEM™ (Gibco by Life Technologies, Cat# 31985-047). The Lonza nucleofector 2b system was used for siRNA transfections. After nucleofection cells were rested for 48 ​h in RPMI supplemented with 10% serum.

The siRNAs (synthesized by Sigma) are shown below:

siNT 5′- AAUUCUCCGAACGUGUCACGU -3´ (non-targeting siRNA control)

siCIP2A-1 5′-CUGUGGUUGUGUUUGCACUTT-3′

Cell fractionation

Human Th17 ​cells, collected 72 ​h post cell activation, were used to isolate cytoplasmic and nuclear fractions using NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Thermo Fisher Scientific, Cat# 78833). Cell fractions were analyzed by western blotting using 4–20% SDS-PAGE Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories, Cat# 4561094). Antibodies, ⍺-Tubulin (Santa Cruz, sc-55529) and ⍺-Vimentin (Santa Cruz, Cat# sc-6260) were used to detect control protein for cytoplasmic and nuclear fractions, respectively. The localization of CIP2A expression in cytoplasm and nuclear fractions was detected using a primary antibody specific for CIP2A (Cell Signaling Technology, Cat# 14805) with 1:1000 dilutions.

LC-MS/MS analysis of the proteomic profiles of the immuno-precipitates

The immuno-precipitates were denatured with 8 M urea and cysteine residues reduced with dithiothreitol then alkylated with iodoacetamide. After digestion with trypsin (37 ⁰C, 16 ​h), the samples were desalted using Empore C₁₈ disks (3M, Cat# 2215). Based on using UV absorption measurements with a NanoDrop-1000 UV spectrophotometer (Thermo Scientific), equivalent aliquots of the digested peptides were analyzed in triplicate with an Easy-nLC 1200 coupled to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). A 20 ​× ​0.1 mm i.d. pre-column was used in conjunction with a 150 ​mm ​× ​75 ​μm i.d. analytical column, both packed with 5 μm Reprosil C₁₈-bonded silica (Dr Maisch GmbH). A separation gradient from 8 to 45% B in 78 ​min was used at a flow rate of 300 nl/min. The mobile phase compositions were, water with 0.1 % formic acid (A) and 80% acetonitrile 0.1% formic acid (B). The MS/MS data were acquired in positive ion mode with a data dependent acquisition setting for higher-energy C-trap dissociation (HCD) of the 10 most intense ions (m/z 300-2000, charge states ​> ​1+). Further details of the preparation and instrument parameters are available as supplementary information. The LC-MS/MS discovery data are available via the PRIDE (Vizcaíno et al., 2016) partner repository from the ProteomeXchange Consortium with the dataset identifier PXD008983.

LC-MS/MS validation of selected targets

Selected reaction monitoring (SRM) mass spectrometry was used to validate the relative abundance of twenty of the proteins that were found to be associated with the CIP2A immuno-precipitates (Supplementary Table 4). Heavy-labeled synthetic peptide (lysine ¹³C₆ ¹⁵N₂ and arginine ¹³C₆ ¹⁵N₄) analogues for the targets of interest were selected on the basis of their consistency and intensity in the discovery data and obtained from Thermo Scientific. For these validations, three additional cultures were prepared from the cord blood of three donors.

Using Skyline software (MacLean et al., 2010), the top five most intense transitions for each peptide were evaluated from the MS/MS spectra of heavy labelled peptides and the relative performance of the native peptides in the spiked validation samples.

The validation samples were prepared using the same digestion and desalting protocols used for discovery. These were then spiked with synthetic heavy labelled analogues of the peptide targets and a retention time standard (MSRT1, Sigma) for scheduled SRM. The LC-MS/MS analyses were conducted using Easy-nLC 1000 liquid chromatograph (Thermo Scientific) coupled to a TSQ Vantage Triple Quadrupole Mass Spectrometer (Thermo Scientific). The column configuration was the same as used in the discovery experiments and the peptides were separated using a gradient of 8% to 43% B in 27 ​min, then to 100% B in 3 ​min, at a flow rate of 300 nl/min (the mobile phase compositions are as indicated above). The raw SRM data are available through PASSEL (Farrah et al., 2012) with the dataset identifier PASS01186.

Data analysis and statistics

The peptide/protein identification was performed in Andromeda search engine (Cox et al., 2011), incorporated in MaxQuant software (Cox & Mann, 2008), with a UniProt human protein sequence database (version June 2016, 20,237 entries). Trypsin digestion with a maximum of two missed cleavages, carbamidomethylation of cysteine as a fixed modification, and methionine oxidation and N-terminal acetylation as variable modifications were used in the search parameters. A false discovery rate (FDR) of 0.01 at the peptide and protein level was applied. MaxQuant's label-free quantification (LFQ) algorithm (Cox et al., 2014) was used to calculate the relative protein intensity profiles across the samples. The “match between the runs” option was enabled with a time window of 0.7 ​min.

The MaxQuant output was further processed using Perseus (Tyanova et al., 2016). The downstream processing included the removal of contaminants, reverse hits and proteins with less than three valid replicate values in the sample groups (IgG or CIP2A pulldown). To identify the potential interacting proteins, Significance Analysis of INTeractome (SAINT) analysis was performed (Mellacheruvu et al., 2013) using the Resource for Evaluation of Protein Interaction Networks (REPRINT) interface (https://reprint-apms.org/). This algorithm uses the distribution, relative intensity and variation of the prey protein detection to calculate probability scores for the certainty of their interaction with the bait. Additionally, the protein lists were compared against a list of common contaminants identified in a catalogue of immunoprecipitation experiments (CRAPome). The thresholds applied to define the putatively interacting proteins included identification on the basis of two or more unique peptides and a SAINT probability score of ≥0.95. Although there was no direct match for our sample type and IP conditions in the contaminants repository database, additional filtering was made to exclude proteins that were more than 60% of the datasets. Skyline was used to design the SRM experiments and process the data generated. The MS Stats (3.8.4) plugin included in the Skyline software was used for the group comparison between cases and controls (Choi et al., 2014).

Network analysis

Previously reported protein-protein interactions for the detected CIP2A interacting proteins were downloaded from the STRING functional protein association networks database version 10.5 (Szklarczyk et al., 2017). Both predicted and known high confidence (interaction score ​≥ ​0.7) interactions were considered. The resulting network was visualized with Cytoscape version 3.6.0 (Shannon et al., 2003). Clusters in the network were identified using the Markov clustering algorithm implemented in the Cytoscape plug-in clusterMaker v2 algorithm MCL Cluster (Morris et al., 2011) with the granularity parameter (inflation value) set to 1.8 as suggested by (Brohée & van Helden, 2006). The MS intensity differences between CIP2A immuno-precipitates and the IgG controls were calculated as median differences across all replicates in the normalized log2-transformed intensities (color coded as a continuous gradient from white to grey as node inner color in Fig. 3A).

Enrichment analysis

The enriched GO biological processes were identified using the Database for Annotation, Visualization and Integrated Discovery (DAVID) version 6.8 (Huang et al., 2009a, Huang et al., 2009b) and the Protein Annotation Through Evolutionary Relationship (PANTHER) classification system version 13.0 (Mi et al., 2017, Mi et al., 2013). The enrichment analyses were performed using the detected CIP2A interactome as the input and the whole detected Th17 proteome from a Th17 profiling study (Tripathi et al., 2019) as the background reference. The GO FAT terms from DAVID, which filter out the broadest, non-specific terms, were considered for calculating the most enriched processes within each identified cluster with more than four members. The GO SLIM terms from PANTHER, which give only the broadest terms and filter the more specific ones, were considered for a summary of the enriched biological processes in the CIP2A interactome. A biological process was considered enriched if it had FDR ≤0.05. To summarize the cellular locations and protein classes the protein list was annotated using Ingenuity Pathway Analysis (Qiagen).

Ethical approval

Usage of the blood of unknown donors is approved by the Ethics Committee, Hospital District of Southwest Finland.

Author contributions

M.M.K. designed and performed the experiments, analyzed data and prepared figures related to WB, TaqMan-PCR and Flowcytometry, and wrote the manuscript. T.V. analyzed data, prepared figures for network analysis and wrote part of the manuscript. M.H.K. designed and performed the experiments, prepared figures, and wrote part of the manuscript. R.M. performed SRM-proteomics, analyzed the proteomics data, and contributed to writing the manuscript. U.U. contributed to the pathway and network analyses and writing of the manuscript. S.D.B performed the proteomics experiments data analysis. E.K. performed experiments, and analyzed the data. J.W. provided scientific expertise, and reagents and edited the manuscript. L.E. designed experiments, supervised the study and edited the manuscript. R.L. designed and supervised the study and wrote the manuscript.

Accession numbers

The mass spectrometry data for the interactome have been deposited with the ProteomeXchange Consortium via the PRIDE (Vizcaíno et al., 2016) partner repository with the dataset identifier PXD008983. The validation data have similarly been submitted to the Peptide Atlas (Desiere et al., 2006) with the identifier PASS01186.

Declaration of Competing Interest

The authors declare that there are no conflict of interest.

Appendix A. Supplementary data

The following are the Supplementary data to this article:

Multimedia component 1

Supplementary Table S1A CIP2A interactome in Th17 cells. (Related to Fig. 1). List of proteins associated with the CIP2A interactome.

Multimedia component 2

Supplementary Table S1B CIP2A interactome in Th17 cells. (Related to Fig. 1). Ingenuity Pathway Analysis (IPA) cellular location and functional class assigned IPA annotation for the proteins detected in the CIP2A interactome.

Multimedia component 3

Supplementary Table S1C CIP2A interactome in Th17 cells. (Related to Fig. 1). Information on CIP2A interactome associated proteins listed together with the data from the MaxQuant results (unique peptides, sequence coverage, intensity and Andromeda score), representation in the CRAPome, and SAINT analysis showing Saint Probability (SP). The data is filtered to only include proteins with a SP values of > 0.95 and less than 60% representation in the CRAPOME.

Multimedia component 4

Supplementary Table S1D CIP2A interactome in Th17 cells. (Related to Fig. 1). The CIP2A interactome detected with individual antibodies specific to CIP2A protein region that is against residues surrounding valine 343 (monoclonal, Ab1), and 618-905 peptide (polyclonal, Ab2).

Multimedia component 5

Supplementary Table S2 (Related to Fig. 3). The 20 most enriched GO FAT biological processes (BP) in each cluster of the CIP2A interactome network in Figure 3A. The GO enrichment was performed using DAVID (Huang et al., 2009a, Huang et al., 2009b) against a Th17 proteome reference background (Tripathi et al., 2019). The GO FAT BP terms, which filter out the broadest higher level GO terms, were considered for more specific enrichment information. For each cluster with four or more members, 20 of the most frequently occurring GO FAT terms among the cluster members are listed. For each term, the number of cluster members (proteins or nodes) with the term, the proportion of cluster members with the term, cluster number and annotation for the cluster in Figure 3A, are shown.

Multimedia component 6

Supplementary Table S3 (Related to Fig. 3). The enriched GO biological processes in the detected CIP2A interactome. The enrichment analysis was performed using PANTHER (Mi et al., 2013, Mi et al., 2017) against a Th17 proteome reference background (Tripathi et al., 2019). The broad GO SLIM terms, which include only the higher level GO terms, were considered for a functional summary of the interactome. Fold enrichment, significance value (p-value) and false discovery rate is presented for each term.

Multimedia component 7

Supplementary Table S4 SRM Targets for the CIP2A interactome validations. (Associated with Fig. 4). The table lists the peptides, associated transitions, collision energies and retention time windows used for the SRM validations of the CIP2A vs. control IgG pull downs.

Multimedia component 8

Supplementary Table S5 SRM MS CIP2A interactome validations. (Associated with Fig. 4). The table lists the log₂ transformed relative abundance data from the SRM analysis of the CIP2A IP vs. control IgG pull downs from three biological replicates for proteins validated for the CIP2A interactome.

Multimedia component 9

Figure S1 (Related to Fig. 1). The polarization of the Th17 cultures used in the study and a schematic representation of the interactome study. (A) The proportion of CCR6+ cells from the flow-cytometry analysis of CCR6 surface expression at 72h in TCR activated control Th0 cells and polarized Th17 cells. The IL17A mRNA expression (B), and IL17A protein secretion (C) from the same cultures of (Fig S1A) are shown. The data (A-C) is from six independent cultures and each culture consisted of cells from more than four individual donors. Error bars represent SEM estimates across the biological replicates and P-values calculated using the paired two-tailed student’s t-test. P <0.01(∗∗) and P <0.001(∗∗∗). (D) A schematic layout representation of the interactome study.

Multimedia component 10

Figure S2 (Related to Fig. 3). The normalized expression of the top CIP2A interacting proteins in selected highly enriched GO biological processes in the detected CIP2A interactome. The 20 strongest CIP2A interacting proteins in the (A) RNA metabolic process (B) RNA splicing via transesterification and (C) RNA splicing via spliceosome enriched GO biological processes. The expression values are normalized log₂ transformed protein intensities from the MS analysis of the CIP2A immuno-precipitates (IP) and the IgG controls. The expression values for both CIP2A antibodies (Ab1 and Ab2) specific to different regions for two individual replicates are shown. The proteins in each heatmap are in descending order based on the median expression differences between the CIP2A-IPs and the IgG controls.

Multimedia component 11

Figure S3 (Related toFig. 4). The averaged logarithmic fold changes and logarithmic significance values (log FDR) for the targeted mass spectrometry (SRM-MS) validations analysis of the twenty selected (shown in Figure 4A) proteins from the CIP2A interactome.

Multimedia component 12

Figure S4 (Related toFig. 5). (A) UBR5 immunostaining in Hela cells and PLA validation for interaction between CIP2A and UBR5 by confocal microscopy. For PLA, the secondary antibodies were used as negative controls. DAPI was used to stain nuclei. Scale bar = 10μm. (B-D) Three replicates of WB validations of selected proteins in CIP2A interactome. CIP2A IP, IgG control IP and Input reactions are shown on the blots. CIP2A IP was performed using either Ab2 (B-C) or both Ab1 and Ab2 antibodies (D) and conformational specific secondary antibody was used to probe the proteins without interference from denatured IgG heavy (50 kDa) and light chains (25 kDa).