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[Preprint]. 2021 Mar 23:2021.03.20.21252680. [Version 1] doi: 10.1101/2021.03.20.21252680

Age-related changes in the upper respiratory microbiome are associated with SARS-CoV-2 susceptibility and illness severity

Jillian H Hurst, Alexander W McCumber, Jhoanna N Aquino, Javier Rodriguez, Sarah M Heston, Debra J Lugo, Alexandre T Rotta, Nicholas A Turner, Trevor S Pfeiffer, Thaddeus C Gurley, M Anthony Moody, Thomas N Denny, John F Rawls, Christopher W Woods, Matthew S Kelly
PMCID: PMC8010748  PMID: 33791716

ABSTRACT

Children are less susceptible to SARS-CoV-2 and typically have milder illness courses than adults. We studied the nasopharyngeal microbiomes of 274 children, adolescents, and young adults with SARS-CoV-2 exposure using 16S rRNA gene sequencing. We find that higher abundances of Corynebacterium species are associated with SARS-CoV-2 infection and SARS-CoV-2-associated respiratory symptoms, while higher abundances of Dolosigranulum pigrum are present in SARS-CoV-2-infected individuals without respiratory symptoms. We also demonstrate that the abundances of these bacteria are strongly, and independently, associated with age, suggesting that the nasopharyngeal microbiome may be a potentially modifiable mechanism by which age influences SARS-CoV-2 susceptibility and severity.

Summary

Evaluation of nasopharyngeal microbiome profiles in children, adolescents, and young adults with a SARS-CoV-2-infected close contact identified specific bacterial species that vary in abundance with age and are associated with SARS-CoV-2 susceptibility and the presence of SARS-CoV-2-associated respiratory symptoms.

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