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. Author manuscript; available in PMC: 2021 May 11.
Published in final edited form as: Nature. 2020 Nov 11;588(7837):296–302. doi: 10.1038/s41586-020-2926-0

Extended Data Figure 1. The reconstituted activity of Split-TurboID in neurons and astrocytes in vitro.

Extended Data Figure 1

a, Schematics of constructs tested. b, Immunoblot analysis of construct expression and biotinylation activity. c, Schematic of neuron-astrocyte mixed-culture assay for Split-TurboID with cell-type specific AAVs in vitro. d, Cultured neurons and astrocytes were infected with AAV1/2-GfaABC1D-TurboID-HA-surface, AAV1/2-hSynI-V5-N-TurboID and/or AAV1/2-GfaABC1D-C-TurboID-HA at DIV14. Representative images of neuron and astrocyte at DIV14 after the treatment of 500 μM biotin for 6h are shown. n= 3 biological repeats.