Figure 5. Phenotypic validation of CD8 T cell responses after a single prime with the Ad5-SARS-2 spike vaccine.

(A) Representative FACS plots showing the frequencies of SARS CoV-2-specific CD8 T cells (K^b VL8) that differentiate into effector memory and central memory T cell subsets. (B) Summary of effector memory and central memory T cell subsets. (C) CD127 expression. (D) CD62L expression. (E) CD44 expression. (F) PD-1 expression. Panels B-F are gated from splenic SARS CoV-2-specific CD8 T cells (K^b VL8). All data are from day 28 post-prime. Data are from one experiment with n=5 per group. Experiment was repeated two additional times with similar results. Indicated P values were determined by Mann-Whitney U test.