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[Preprint]. 2021 Apr 1:2021.03.31.437931. [Version 1] doi: 10.1101/2021.03.31.437931

Figure 6. A LD prime elicits CD8 T cell responses with intrinsically superior anamnestic capacity.

Figure 6.

CD45.2+ mice were immunized intramuscularly with 106 or 109 PFU of Ad5-SARS-2 spike, and at day 28, splenic CD8 T cells were MACS-sorted. Subsequently, live, CD8+, CD44+, Kb VL8+ cells were FACS-sorted to ~99% purity, and numbers were normalized for adoptive transfer into CD45.1+ recipient mice. (A) Experimental approach for evaluating secondary expansion of donor CD8 T cells. (B) Representative FACS plots showing the frequencies of donor CD8 T cells after boosting. (C) Summary of donor-derived CD8 T cells in PBMCs. (D) Summary of donor-derived CD8 T cells in spleen. (E) Summary of donor-derived CD8 T cells in draining lymph nodes. Data from panels B-E are from day 14 post-boost. Data are from one experiment with n=5 per group. Experiment was repeated once with similar results. Indicated P values were determined by Mann-Whitney U test.