In the September 2015 issue of Human Gene Therapy (vol. 26, no. 9; 593–602) the article entitled Stability and Safety of an AAV Vector for Treating RPGR-ORF15X-Linked Retinitis Pigmentosa, by Deng, et al. requires correction. The original version of this article contained errors in the sequences in Fig. 2 and the figure and legend have been replaced. Also the corresponding text in the Results section of the original article has been corrected accordingly.
The original Figure 2 and legend read as follows:
Figure 2.
Amino acid sequence alignment of AAV-hIRBP-hRPGR, and AAV-hGRK1-hRPGR with reference to the GenBank hRPGR sequence in the ORP15 domain where variations exist. (–) indicate amino acid deletion. Amino acids that are different are in red.
The updated Figure 2 and legend reads:
Figure 2.
Amino acid sequence alignment of AAV-hIRBP-hRPGR, and AAV-hGRK1-hRPGR with reference to the GenBank hRPGR sequence in the ORP15 domain where variations exist. (–) indicate amino acid deletion. Amino acids that are different are in red.
A part of the first paragraph of the Results section under the heading AAV-hIRBP-hRPGR and AAV-hGRK1-hRPGR plasmids contain sequence variations within ORF15, located on page 598, originally read as follows:
All the differences are within the ORF15 domain between nucleotides 2461 and 3057 (Fig. 2). Specifically, the clone contained 7 deletions resulting in a loss of 45 basepairs (bp) but retaining the original reading frame, one 3 bp insertion, and 65 bp substitutions spread throughout the AG-rich region. These changes translate into 15 amino acid deletions, 1 amino acid insertion, and 26 amino acid substitutions. We hypothesize that these in-frame changes within the AGrich region may not affect normal RPGR function because of its natural splicing complexity within this region, and that this ORF15 transcript retains an intact C-terminal LELK amino acid sequence.11
The section has been updated to read:
“All the differences are within the ORF15 domain between amino acid 821 and 1006 (Fig. 2). Specifically, the clone contained 16 amino acid deletions, 2 amino acid insertions, and 15 amino acid substitutions. Most importantly, the clone retained the original reading frame. We hypothesize that these in-frame changes within the AG rich region may not affect normal RPGR function because of its natural splicing complexity within this region, and that this ORF15 transcript retains an intact C-terminal LELK amino acid sequence.11”
The online version of this article has been corrected to reflect the corrected Figure 2 and legend and the updated Results section.
The authors apologize for this oversight.