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Exosomal Siglec-11 can be secreted. (A) Schematic representation of the F1-GFP protein and expression plasmid. (B) Cell lysates and culture medium of V5-tagged hSiglec-11(4D) and F1-GFP cotransfected HEK293 cells transiently for 5 days were lysed and analyzed by western blotting with anti-V5 tag antibody [hSiglec-11(4D)], anti-GFP (F1-GFP), anti-flotillin-1 (lipid raft/exosome marker) and β-actin (loading control). SH-SY5Y cells cultured with active/inactive Endo-NF.
