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. 2020 Aug 21;35(4):1073–1086. doi: 10.1038/s41375-020-01025-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a FC analysis of MEC-1 cells transduced with lentiviral particles coding for SLAMF1 or SLAMF7. b Proliferation of SLAMF1 or 7 overexpressing MEC-1 cells after 120 h compared to control cell line transduced with empty vector. N = 12. c FC analysis of MEC-1 cells after knockout of SLAMF1 or 7 using CRISPR/Cas9 technology. d Proliferation of MEC-1cells depleted of SLAMF1 or 7 after 120 h compared to control cell line transduced with a non-targeting (scr) gRNA. N = 9. e FC analysis of MEC-1 cells after the subsequent knockout of both, SLAMF1 and SLAMF7. f Proliferation of MEC-1 cells after knockout of SLAMF1 and SLAMF7 after 120 h compared to control cell line. N = 9. Data from independent experiments are shown as mean, error bars represent SEM, statistical significance was calculated by one-way ANOVA and Bonferroni’s post-hoc test. FC flow cytometry, SEM standard error of the mean.