Antibodies |
Mouse anti-OT neurophysin |
Whitnall et al., 1985
Ben-Barak et al., 1985; gift from Harold Gainer, PhD |
PS38 |
Alexa 488 donkey anti-mouse |
Life Technologies |
A-21202 |
Alexa 647 donkey anti-mouse |
Life Technologies |
A-31571 |
Alexa 647 goat anti-mouse |
Life Technologies |
A-21235 |
Alexa 350 goat anti-mouse |
Life Technologies |
A-11045 |
Rabbit anti-FluoroGold |
Fluorochrome |
NA |
Alexa 647 donkey anti-rabbit |
Life Technologies |
A-31573 |
Chicken anti-GFP |
ABCAM |
Ab13970 |
Alexa 488 goat anti-chicken |
Life Technologies |
A-11039 |
Goat anti-mCherry |
SICGEN |
AB0040 |
Alexa 555 donkey anti-goat |
Life Technologies |
A-21432 |
Mouse anti-FMRP |
Developmental Studies Hybridoma Bank |
2F5-1 |
Alexa 647 goat anti-mouse IgG2b |
Jackson ImmunoResearch |
115-607-187; RRID: AB 2632546 |
Rabbit anti-OT |
Altstein and Gainer, 1988; gift from Harold Gainer, PhD |
VA10 |
Alexa 488 donkey anti-rabbit |
Life Technologies |
A-21206 |
|
|
|
Bacterial and Virus Strains |
rgAAV-EF1 a-fDIO-Cre |
Schneeberger et al., 2019 |
Addgene 121675- AAVrg |
rgAAV-CAG-fDIO-GFP-Cre |
Stanford University Neuroscience Gene Vector and Virus Core |
N/A |
CAV-2-GFP |
Plateforme de Vectorologie de Montpellier |
N/A |
CAV-2-Cre-GFP |
Plateforme de Vectorologie de Montpellier |
N/A |
|
|
|
Chemicals, Peptides, and Recombinant Proteins |
Neurobiotin |
Vector Laboratories |
SP-1120 |
Alexa 350 streptavidin conjugate |
Life Technologies |
S11249 |
FluoroGold |
Fluorochrome |
N/A |
Red Retrobeads |
Lumafluor |
N/A |
4-Aminopyridine |
Sigma-Aldrich |
275875 |
Cocaine hydrochloride |
Sigma-Aldrich |
C5776 |
|
|
|
Critical Commercial Assays |
Worthington Papain Dissociation System |
Worthington Biochemical Corporation |
Cat#LK003182
|
Illumina TruSeq kit |
Illumina |
Cat#RS-122-200 |
|
|
|
Deposited Data |
Accession number GSE147092
|
GEO |
|
|
|
|
Experimental Models: Organisms/Strains |
C57BL6/J |
Jackson Laboratories |
Stock # 000664 |
Calbl-IRES-Cre |
Jackson Laboratories |
Stock # 028532 |
Flp-dependent GFP reporter mice |
Mutant Mouse Resource Center |
Stock # 32038 |
Ai9 Cre-dependent TdTomato reporter mice |
Jackson Laboratories |
Stock # 007909 |
Constitutive Fmr1 knockout |
Jackson Laboratories |
Stock # 003025 |
Conditional Fmr1 knockout |
Mientjes et al., 2006 |
N/A |
Oxytocin-2A-Flp-optimized |
Nardou et al., 2019 |
N/A |
|
|
|
Oligonucleotides |
see supplemental tables (HCR) |
|
|
|
|
|
Recombinant DNA |
fDIO-Cre-GFP |
Penzo et al., 2015 |
Dr. Bo Li and Dr. Linda Van Aelst |
|
|
|
Software and Algorithms |
R version 3.5 |
The R project |
https://www.r-project.org/ |
MATLAB |
Mathworks |
https://www.mathworks.com/ |
Prism 5 |
GraphPad |
https://www.graphpad.com/ |
Igor Pro |
WaveMetrics |
https://www.wavemetrics.com/ |
Recording Artist (plugin for Igor Pro) |
Richard C Gerkin, PhD |
https://github.com/rgerkin/recording-artist |