Fig. 3.

Knockdown of Axin posteriorizes sea urchin embryos. (A) Top panel shows a control pluteus-stage larva and the bottom panel shows an Axin-knockdown embryo at the same stage. (B) Gene expression in control and Axin-knockdown embryos at the hatching blastula stage analyzed using qPCR. The bar graph shows the fold change in expression of each gene between Axin MO- and control MO-injected embryos. Blimp1, Hox11/13b, Endo16, Brachyury, FoxA, GataE, Alx1, Delta and GCM are endomesoderm gene markers; Foxq2 and Six3 are ANE markers. qPCR experiments were replicated three times with three technical replicates for each experiment. Dashed line indicates a two-fold change. Data are mean±s.e.m. *P<0.05. Scale bars: 10 µm. Experiments were carried out in S. purpuratus.