Figure 5.

Evaluation of oxidative stress mediators after HMEC-1 treatment with AHE or control. Antioxidant SOD (A) and catalase (B) defenses were both augmented at concentrations of 20 and 25 mg/L, in comparison with control group, analyzed by enzymatic assays. Production of reactive oxygen species (ROS) was reduced dose-dependently, measured after incubation with fluorescence dies, with statistical significance at 15 mg/L or higher (C). Results are presented as % inhibition rate of SOD activity (A) nmol of catalase activity/min/mL (B) and units of fluorescence intensity for ROS production (C) * p < 0.05 vs. control.