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. 2021 Mar 30;26(7):1946. doi: 10.3390/molecules26071946

Figure 3.

Figure 3

The effects of alkaloids on intracellular reactive oxygen species (ROS) level in 2.5 μM Dox-induced H9c2 cells. The cells were pretreated with different concentrations of alk-A and alk-B (10, 20, and 40 µM) for 1 h, then exposed to Dox for 24 h. CCCP (carbonyl cyanide 3-chlorophenylhydrazone) was used as a negative contrast group. Results are mean ± SEM from three independent experiments. DCFH-DA(2,7-Dichlorodi -hydrofluorescein diacetate) is a fluorescent probe to measure ROS level. ## p < 0.01 vs. control, * p < 0.05, ** p < 0.01 vs. Dox group.