Figure 3.

MAIT17 cells are reduced in the absence of Bcl11b in the thymus and periphery and have reduced IL17A production at steady state

(A) Representative dot plots of RORγt and Tbet within stage 3 thymic TCRβ+MR1t-5-OP-RU + cells of Bcl11b^F/F/PLZFcre and WT mice.

(B) Frequencies of RORγt + Tbet-, RORγt-Tbet+, RORγt + Tbet+, and RORγt-Tbet-within developmental stage 3 TCRβ+MR1t-5-OP-RU + MAIT cells of Bcl11b^F/FPLZFcre and WT mice. The data are derived from 8 pairs of mice in which two FACS files from the same type of mice (Bcl11b^F/F/PLZFcre or WT mice) were concatenated.

(C) Representative dot plots of RORγt and Tbet within lin-TCRβ+MR1t-5-OP-RU+ in the lung of Bcl11b^F/F/PLZFcre and WT mice.

(D) Frequencies of Bcl11b KO and WT RORγt+Tbet-, RORγt-Tbet+, RORγt+Tbet+ and RORγt-Tbet-lin-TCRβ+MR1t-5-OP-RU + MAIT cell subsets in the lung.

(E) Representative dot plots of Bcl11b KO and WT RORγt+ IL17A+ lin-TCRβ+MR1t-5-OP-RU+ MAIT cells in lung.

(F) Frequencies of Bcl11b KO and WT RORγt+ IL17A+ TCRβ+MR1t-5-OP-RU+ MAIT cells in the lung. Each point represents a concatenated file of 2 lung samples run independently.

Data are represented as mean ± SEM. All statistics were conducted as paired t test. p < 0.05 was considered significant.