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. 2021 Apr 12;9:88. doi: 10.1186/s40168-021-01043-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — T6SS1 in different species and strains. A multiple alignment of the T6SS1 region and 35Kb upstream and downstream were evaluated for 4 reference genomes. The figure shows the collinear blocks identified between the clinical strain (RIMD 2210633), the one that does not produce AHPND (NCKU_TN_S02) and the one that produces AHPND (BA94) of V. parahaemolyticus, and the strain of V. diabolicus ILI. Each gene from the T6SS1 is represented in the top segment with different colors, conservation of each gene and the upstream and downstream regions can be traces by the vertical lines. The ILI strain contains at least partially T6SS1, and the other part is found on chromosome II, which is why we call it T6SS2B. All V. parahaemolyticus strains analyzed in this study contain a highly conserved T6SS1. T6SS1 in ILI contains the effectors described for V. parahaemolyticus by Salomon et al. [47] shown in blue, purple, and orange boxes