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. 2021 Apr 12;9:89. doi: 10.1186/s40168-021-01036-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Biological characterisation of ΦcrAss002. a Transmission electron micrograph, generated from ΦcrAss002 enriched lysate, stained with uranyl acetate. Micrographs show podovirus-like virions with a diameter of ~77 nm and a simple tail structure. Scale bars represent 100 nm. b In vitro propagation of ΦcrAss002 over 11 days. Titre quantification was performed using qPCR. c Investigation of the ability of ΦcrAss002 to propagate on commercial B. xylanisolvens DSM18836 and the only other confirmed crAss-like phage host to date, B. intestinalis 919/174 via liquid propagation over 5 days. Error bars indicate standard deviation (n = 3)