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Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1999 Dec 1;40(4):315–321. doi: 10.1186/BF03547011

Identification of Coagulase-Positive Staphylococci Isolated from Bovine Milk

Identifiering av koagulaspositiva stafylokocker isolerade från bovin mjölk

A Capurro 1, C Concha 2,✉, L Nilsson 2, K Östensson 1
PMCID: PMC8043227  PMID: 10918901

Abstract

A total of 414 coagulase-positive staphylococcal strains obtained at the mastitis laboratory, National Veterinary Institute, Uppsala, Sweden, were studied. One hundred and seventy seven strains were used for a frequency study. Ninety-seven per cent were identified as Staphylococcus aureus, 2% as Staphylococcus intermedius and 1% as Staphylococcus hyicus. Two hundred and thirty seven strains with atypical hemolysis reactions on bovine blood agar were randomly selected, with the aim to increase the number of S. intermedius and S. hyicus strains available for testing. Eight different characteristics, including physiological, enzymatical and biochemical properties, were used to identify the coagulase-positive Staphylococcus species. The results of this study suggest that the following tests should be included for correct identification of the 3 different species of coagulase-positive staphylococci: P agar supplemented with acriflavin, β-galactosidase and hemolytic reaction on chocolate agar. These 3 tests are simple and quick to perform and enable accurate for easy differentiation of the 3 coagulase-positive Staphylococcus species.

Keywords: cow, mastitis, differentiation

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