Figure 6.

Distinct features of severe versus moderate MIS-C

(A) PCA of TRBV usage in CD4⁺ and CD8⁺ memory cells, along with frequency of TRBV11-2 usage, in the pediatric cohort. Statistical significance for PCA calculated by permutation test, and by one-sided Wilcoxon test for TRBV11-2 frequency comparisons.

(B) PRF1 and GZMA expression in effector memory CD8⁺ T cells along with dot plot depicting relative average expression and percent expression for four cytotoxicity genes (right). Two-sided Wilcoxon rank sum tests were used to calculate significance.

(C) Flow cytometric evaluation of Granzyme A in TEMRA CD8⁺ compartment in C.HD (n = 6), MIS-C-S (n = 7), and MIS-C-M (n = 5) patients. Statistical significance was assessed using an ordinary one-way ANOVA test.

(D) Correlation between BCR diversity and TCR diversity relating to combined Ki67⁺ and memory CD4⁺ T cells. P7.1 was excluded from TCR analysis due to low cell numbers (see STAR Methods).

(E) B cell diversity, plasmablast frequency, and proportion of mutated IGHG within MIS-C cohort.

(F) Serum E-selectin in pediatric healthy and MIS-C donors. Statistical significance was calculated as in (B).

(G) Median fluorescence intensity (normalized to average HD) of serum IgG binding to cultured human cardiac microvascular endothelial cells (HCMEC) by flow cytometry (left). A non-parametric two-sided Wilcoxon rank sum test was used to calculate significance. Error bars represent mean with SD. MIS-C-S (acute n = 3; P1-3); MIS-C-M (acute n = 2; P4-5 and recovered n = 1; P11); and HD (n = 6; 1 C.HD and 5 A.HD). Representative histogram of a patient representing the median of the MIS-C-S cohort and sampled prior to IVIG treatment (right).