Corrigendum: Role of HK2 in the Enzootic Cycle of Borrelia burgdorferi

In the original article, there was a mistake in Figure 5 as published. Due to an error in compiling multi-panel images, a gap between the image of “B31-A3” and the image of “B31A3/flaBp-HD-GYP; B31A3/flaBp-hk2” was omitted. In this figure, unphosphorylated Rrp2 (lower lane) serves as an internal control for each sample. Overproduction of an unrelated protein HD-GYP (B31A3/flaBp-HD-GYP) serves as the negative control for overproduction of Hk2 (B31A3/flaBp-hk2), showing a reduction of Rrp2 phosphorylation by overexpression of Hk2. The corrected Figure 5 appears below.

Figure 5.

Overexpressing HK2 reduces the level of phosphorylated Rrp2 in B. burgdorferi. Phos-tag SDS-PAGE and immunoblotting was used to detect both phosphorylated and dephosphorylated Rrp2 in the cell. Wild-type B. burgdorferi B31A3, B31A3 carrying a shuttle vector harboring a unrelated protein HD-GYP (B31A3/flaBp-HD-GYP), or B31A3 carrying a shuttle vector harboring a hk2 gene driven by a flaB promoter GYP (B31A3/flaBp-hk2), were harvested at mid-log phase and cell lysates were prepared and separated on 7.5% SDS-PAGE containing 0, 5, 10, and 25 uM Phos-tag followed by immunoblotting using anti-Rrp2 antibody. p-Rrp2, the band corresponds to phosphorylated Rrp2. As a unphosphorylated Rrp2 control, B31A3 was also treated by boiling (lane 2) prior to Phos-tag SDS-PAGE (Rrp2 phosphorylation is unstable and sensitive to heat).

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.