Table 2.

 Construction of MYB5a/NEGAN RNAi transgenes for use in M. l. variegatus

Insert	Insert length (bp)	Primers
M. l. variegatus 5′-UTR fragment	307	24 F: caccCAAATTTTGGTTTTTGCCATTT
		37 R: TCGGTCAAATTAAATGCACA
M. l. variegatus exon 4 fragment	377	60 F: caccGAAGAACGGCCAACGAC
		56 R: GCAGCTTCCCCGTTTCA

Both inserts were cloned into the pB7GWIWG2(I) RNAi vector. Primers are labeled with a number and F for Forward or R for Reverse. Their sequences are shown 5′ to 3′. Nucleotides added to primers for purposes of directional cloning or restriction enzyme digests are underlined. bp, base pairs. Lowercase cacc- represents nucleotides added to primers to enable cloning into the pENTR entry vector.