Table 3.
Western blot analysis of intracellular signaling pathways in massaged limb (WBM or HSM) and contralateral non-massaged limb (WBM-L or HSM-L) in response to massage
| WB | WBM | HS7 | HSM | WBM-L | HSM-L | |
|---|---|---|---|---|---|---|
| Total Akt | 0.94 ± 0.04 | 1.00 ± 0.12 | 1.23 ± 0.12 | 1.28 ± 0.09 | 1.09 ± 0.07 | 1.31 ± 0.05 |
| Phospho-Akt | 0.97 ± 0.14 | 1.31 ± 0.23 | 1.12 ± 0.07 | 1.00 ± 0.15 | 1.01 ± 0.15 | 1.10 ± 0.17 |
| Total FOXO3A | 1.04 ± 0.15 | 0.92 ± 0.09 | 1.05 ± 0.09 | 1.01 ± 0.09 | 0.98 ± 0.08 | 1.19 ± 0.06 |
| Phospho-FOXO3A | 1.08 ± 0.13 | 1.10 ± 0.09 | 1.20 ± 0.08 | 1.30 ± 0.12 | 1.33 ± 0.09 | 1.32 ± 0.08 |
| Total FAK | 4.74 ± 0.61 | 4.38 ± 0.70 | 4.25 ± 0.73 | 4.68 ± 0.59 | 3.89 ± 0.56 | 4.92 ± 0.52 |
| Phospho-FAK | 0.87 ± 0.05 | 1.15 ± 0.15 | 1.00 ± 0.12 | 1.11 ± 0.09 | 0.92 ± 0.12 | 0.94 ± 0.08 |
| Total ERK1/2 | 1.01 ± 0.07 | 1.07 ± 0.03 | 1.19 ± 0.09 | 1.26 ± 0.04 | 1.24 ± 0.10 | 1.11 ± 0.06 |
| Phospho-ERK1/2 | 1.07 ± 0.05 | 1.08 ± 0.05 | 1.08 ± 0.05 | 1.09 ± 0.05 | 1.08 ± 0.05 | 1.13 ± 0.06 |
| Total eEF2 | 1.19 ± 0.10 | 1.18 ± 0.07 | 1.14 ± 0.05 | 1.06 ± 0.07 | 1.24 ± 0.08 | 1.13 ± 0.04 |
| Phospho-eEF2 | 1.05 ± 0.07 | 1.15 ± 0.06 | 0.91 ± 0.09 | 1.03 ± 0.07 | 1.08 ± 0.08 | 1.16 ± 0.12 |
| Total S6K1 | 1.11 ± 0.06 | 1.03 ± 0.09 | 0.95 ± 0.06 | 1.05 ± 0.04 | 1.12 ± 0.02 | 1.04 ± 0.05 |
| Phospho-S6K1 | 1.51 ± 0.21 | 1.47 ± 0.25 | 0.91 ± 0.17 | 0.63 ± 0.07 | 1.76 ± 0.39 | 0.74 ± 0.20 |
| Total 4EBP1 | 0.90 ± 0.02 | 0.91 ± 0.04 | 1.18 ± 0.08 | 1.18 ± 0.07 | 0.87 ± 0.04 | 1.09 ± 0.07 |
| Phospho-4EBP1 | 0.82 ± 0.08 | 0.77 ± 0.07 | 1.19 ± 0.14 | 1.10 ± 0.07 | 0.72 ± 0.07 | 1.22 ± 0.11 |
| Total rpS6 | 1.08 ± 0.15 | 1.10 ± 0.18 | 0.89 ± 0.15 | 0.87 ± 0.10 | 0.84 ± 0.04 | 0.77 ± 0.13 |
| Phospho-rpS6 | 1.33 ± 0.16 | 1.17 ± 0.19 | 0.80 ± 0.16 | 0.72 ± 0.19 | 1.15 ± 0.09 | 0.63 ± 0.17 |
| Total MuRF-1 | 1.04 ± 0.11 | 1.02 ± 0.09 | 1.06 ± 0.09 | 1.10 ± 0.09 | 1.05 ± 0.06 | 1.06 ± 0.06 |
| Total UBF-1 | 0.81 ± 0.13 | 0.91 ± 0.08 | 0.72 ± 0.07 | 0.85 ± 0.08 | 0.79 ± 0.12 | 0.78 ± 0.11 |
| Phospho-UBF-1 | 1.28 ± 0.11 | 1.15 ± 0.08 | 0.97 ± 0.12 | 1.06 ± 0.06 | 1.28 ± 0.13 | 1.01 ± 0.10 |
| Total c-Myc | 1.44 ± 0.13 | 1.16 ± 0.19 | 1.30 ± 0.21 | 1.31 ± 0.21 | 1.11 ± 0.19 | 0.97 ± 0.18 |
Total and phosphorylated analysis of signaling proteins for WB (n = 7), WB massaged limb WBM (n = 8), HS for 7 days HS7 (n = 8), HS massaged limb HSM (n = 8), WB non-massaged contralateral limb WBM-L (n = 8), and HS non-massaged contralateral limb HSM-L (n = 8). Two-tailed independent sample t tests between the respective control (WB or HS) and either the massaged limb (WBM or HSM) or contralateral non-massaged limb (WBM-L or HSM-L) was used to determine statistical significance. All values are in arbitrary density units. Values are mean ± SEM. Representative images for the data within this table are in Supplemental Fig. 1