Skip to main content
PMC home page
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1998 Jun 1;39(2):201–213. doi: 10.1186/BF03547793

The Effect of Prednisolone on Canine Neutrophil Function: In Vivo and in Vitro Studies

In vivo- och in vitroeffekter av prednisolon på granulocytfunktioner hos hund

G Trowald-Wigh 16,✉, L Håkansson 26, A Johannisson 36,46, LE Edqvist 56
PMCID: PMC8050686  PMID: 9787484

Abstract

The in vivo effect of a therapeutic dose of prednisolone on canine neutrophil adherence, random migration, Chemotaxis, phagocytosis of IgG and C3b opsonized yeast cells, chemiluminescence, Fc- and CR3-receptor expression was investigated. Prednisolone was also added in vitro to neutrophils as isolated cells and in whole blood. In the in vivo study, prednisolone increased the IgG mediated ingestion of yeast cells and the number of activated neutrophils in the phagocytosis assay, while flow cytometric investigation of the IgG-receptor FcγRIII with a monoclonal antibody showed similar expression before, during and after treatment. Prednisolone also increased the ingestion of C3b-opsonized yeast cells, while the expression of CR3-receptors (CD11b CD18) measured by flow cytometry was unchanged. Chemiluminescence and the chemotactic response towards zymosan activated serum were increased, while adherence to nylon wool was decreased. The in vitro studies revealed that prednisolone had no or a dampening effect on neutrophils in cell suspensions. Adherence as well as IgG mediated ingestion was decreased at the highest prednisolone concentration (800 ng/ml) in whole blood. The present study suggests that the part of the antiinflammatory effect of corticosteroids mediated through their influence on neutrophils, besides reduced adherence, may be exerted by increased clearance of microorganisms and IgG-complexes through an elevated functional capacity.

Keywords: adhesion, migration, Chemotaxis, phagocytosis, FcγIII-receptor-expression, CR3- receptor expression, corticosteroids

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Acknowledgments

The authors wish to thank Ulla Nestor for excellent and skillful laboratory assistance. We also wish to thank Inger Sjöstedt for hematological analysis and Åsa Karlsson for serum cortisol/prednisolone determinations. This study was supported by grants from Agria Insurance Company, Stockholm, Sweden. The flow cytometry equipment was financed by the Knut & Alice Wallenberg foundation.

References

  1. Baltch AL, Hammer MC, Smith RP, Bishop MB, Suth-pen NT, Egy MA, Michelsen PB. Comparision of the effect of three adrenal corticosteroids on human granulocyte function against Pseudomonas aeruginosa. The Journal of Trauma. 1986;26:525–533. doi: 10.1097/00005373-198606000-00006. [DOI] [PubMed] [Google Scholar]
  2. Barr RD, Koekebakker M, Milner RA. Hydrocorti-sone-A possible regulator of human granulopoiesis. Scand. J. Hematol. 1983;31:31–38. doi: 10.1111/j.1600-0609.1983.tb02133.x. [DOI] [PubMed] [Google Scholar]
  3. Clark RAF, Gallin JI, Fauci AS. Effects of in vivo prednisone on in vitro eosinophil and neutrophil adherence and Chemotaxis. Blood. 1979;53(4):633–641. doi: 10.1182/blood.V53.4.633.633. [DOI] [PubMed] [Google Scholar]
  4. Colburn WA, Sibley CR, Buller RH. Comparative serum prednisone and Prednisolon concentrations following prednisone or prednisolone administration to beagle dogs. J. Pharm. Sci. 1979;65(7):997–1001. doi: 10.1002/jps.2600650711. [DOI] [PubMed] [Google Scholar]
  5. Cupps TR, Fauci AS. Corticosteroid-Mediated im-munoregulation in man. Immunol. Rev. 1982;65:133–155. doi: 10.1111/j.1600-065X.1982.tb00431.x. [DOI] [PubMed] [Google Scholar]
  6. Feldman EC, Nelson RW: Canine and Feline endocrinology and reproduction. Darlene Pedersen (editor). Saunders Company. 1987,218–227.
  7. Forslid J, Hed J. Hydrocortisone both decreases the up-regulation of complement receptors CR1 and CR3 and the ingestion process of human granulocytes. Int. Archs. Allergy appl. Immun. 1987;84:345–350. doi: 10.1159/000234448. [DOI] [PubMed] [Google Scholar]
  8. Guelfi JF, Courdouhji MK, Alvinerie M, Toutain PL. In vivo and in vitro effects of three glucocorticoids on blood leucocyte Chemotaxis in the dog. Vet. Immunol. Immunopathol. 1985;10:245–252. doi: 10.1016/0165-2427(85)90050-9. [DOI] [PubMed] [Google Scholar]
  9. Hed J. The extinction of fluorescence by chrystal violet and its use to differentiate between attached and ingested microorganisms in phagocytosis. FEMS Lett. 1977;1:357–361. doi: 10.1111/j.1574-6968.1977.tb00651.x. [DOI] [Google Scholar]
  10. Hirsch GJ, Church AB. Adrenal steroids and infection: the effect of cortisone administration on polymorphonuclear leucocytic functions and on serum opsonins and bactericidins. J. Clin. Invest. 1961;40:794–798. doi: 10.1172/JCI104312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inada K, Ogasawara M, Suzuki M, Kirikae T, Mori KJ, Yoshida M. Enhancement of in-vitro granulopoiesis and induction of differentiation of Ml cells by a glucocorticoid-dependent factor produced from P388D1 cells. Leukemia Research. 1988;12:305–314. doi: 10.1016/0145-2126(88)90045-8. [DOI] [PubMed] [Google Scholar]
  12. Kuzniar J, Sajewicz K W, Hruby Z. Chemilumi-nescence of neutrophils in patients with glomeru-linephritis treated with methylprednisolone. International Urology and Nephrology. 1991;23:527–534. doi: 10.1007/BF02583999. [DOI] [PubMed] [Google Scholar]
  13. Levine PH, Hardin JC, Scoon KL, Krinsky NI. Effect of corticosteroids on the production of superoxide and hydrogen peroxide and the appearance of chemiluminescence by phagocytosing polymorphonuclear leucocytes. Inflammation. 1981;5:19–27. doi: 10.1007/BF00910776. [DOI] [PubMed] [Google Scholar]
  14. Lewis SL, van Epps DE. Demonstration of specific receptors for fluoresceinated casein on human neutrophils and monocytes using flow cytometri. Inflammation. 1983;7:363–375. doi: 10.1007/BF00916301. [DOI] [PubMed] [Google Scholar]
  15. Liles WC, Dale DC, Klebanoff SJ. Glucocorticoids inhibit apoptosis of human neutrophils. Blood. 1995;86:3181–3188. doi: 10.1182/blood.V86.8.3181.3181. [DOI] [PubMed] [Google Scholar]
  16. MacGregor RR, Spagnuolo PJ, Lentnek AL. Inhibition of granulocyte adherence by ethanol, prednisone and aspirin measured with an assay system. N. Engl. J. Med. 1974;291:642–646. doi: 10.1056/NEJM197409262911302. [DOI] [PubMed] [Google Scholar]
  17. Mandell GL, Rubin W, Hook EW. The effect of on NADH oxidase inhibitor (hydrocortisone) on polymorphonuclear leucocyte bactericidal activity. J. Clin. Invest. 1970;49:1381–1388. doi: 10.1172/JCI106355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Molina JM, Garrido A, Anguiano A, Uceda A, Serrano E. Experimental study of the effect of 6-methyl-prednisolone on ingestion-microbicidal function activity in dog neutrophils. Comp. Immun. Microbiol. Infect. Dis. Vol. 1991;14:21–29. doi: 10.1016/0147-9571(91)90037-E. [DOI] [PubMed] [Google Scholar]
  19. Morris DD, Strzemienski PJ, Gaulin G, Spencer P. The effect of corticosteroid administration on the migration, phagocytosis and bactericidal capacity of equine neutrophils. Cornell Vet. 1988;78:243–252. [PubMed] [Google Scholar]
  20. Motomura S, Katsuno M, Kaneko S, Ibayashi H. The effect of hydrocortisone on the production and differentiation of granulocyte/macrophage progenitor cells in long-term bone marrow cultures. Exp. Hematol. 1983;11:56–62. [PubMed] [Google Scholar]
  21. Niwa Y, Somiya K, Miyachi Y, Kanoh T, Sakane T. Luminol independent chemiluminescence by phagocytes is markedly enhanced by dexametha-sone, not by other corticosteroids. Inflammation. 1987;11:163–174. doi: 10.1007/BF00916018. [DOI] [PubMed] [Google Scholar]
  22. Olds J W, Reed WP, Eberle B, Kisch AL. Corticosteroids, serum and phagocytosis: In vitro and in vivo studies. Infect, and Immun. 1974;9:524–529. doi: 10.1128/IAI.9.3.524-529.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Phillips TR, Yang WC, Schulz RD. The effects of glu-cocorticosteroids on the chemiluminescence response of bovine phagocytic cells. Vet. Immunol, and Immunopathol. 1987;14:245–256. doi: 10.1016/0165-2427(87)90093-6. [DOI] [PubMed] [Google Scholar]
  24. Roth JA, Kaeberle ML. Effects of in vivo dexametha-sone administration on in vitro bovine polymorphonuclear leucocyte function. Infection and Immunity. 1981;33:434–441. doi: 10.1128/IAI.33.2.434-441.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Suda T, Miura Y, Ijima H, Ozawa K, Motoyoshi K, Takuku F. The effect of hydrocortisone on human granulopoeisis in vitro with cytochemical analysis of colonies. Exp. Hematol. 1983;11:114–121. [PubMed] [Google Scholar]
  26. Trowald-Wigh G, Håkansson L, Johannisson A, Nor-rgren L. Hård af Segerstad, C. Leucocyte adhesion protein deficiency in Irish setter dogs. Veterinary Immunology and Immunopathology. 1991;32:261–280. doi: 10.1016/0165-2427(92)90050-z. [DOI] [PubMed] [Google Scholar]
  27. Trowald-Wigh G, Thorén-Tolling K. Phagocytosis induced by yeast cells in canine granulocytes: A methodological study. Acta Vet. Scand. 1990;31:87–95. doi: 10.1186/BF03547581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Trowald-Wigh G, Thorén-Tolling K. Chemilumines-cence and Chemotaxis assay of canine granulocytes: A methodological study. Acta Vet Scand. 1990;31:79–86. doi: 10.1186/BF03547580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Webster RO, Hong SR, Johnston RB, Jr, Henson PM. Biological effects of the human complement fragments C5a and C5a des Arg on neutrophil function. Immunopharmacology. 1980;2:201–219. doi: 10.1016/0162-3109(80)90050-8. [DOI] [PubMed] [Google Scholar]
  30. Wilkinsson PC. Chemotaxis and inflammation. London: Churchill Livingstone; 1974. [Google Scholar]
  31. Zigmond SH, Hirsch JG. Leucocyte locomotion and Chemotaxis. New methods for evaluation and demonstration of a cell derived chemotactic factor. J. Exp. Med. 1973;137:387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES