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. 2021 Mar 26;10:e67262. doi: 10.7554/eLife.67262

Figure 5. Lateral entorhinal cortex-layer Vb (LEC-LVb) neurons target layer Va (LVa) pyramidal neurons as well as LII/III pyramidal neurons.

(A) Representative image of semicoronal slice showing expression of oChIEF-citrine in LVb neurons (green) and recorded neurons labeled with biocytin (magenta) in LEC. Inset shows a low-power image of the section indicating the position of the higher-power image. Scale bars represent 500 μm (inset) and 100 μm. (B) Voltage responses to injected current steps recorded from neurons shown in (A): i, pyramidal cell in LVa; ii, pyramidal cell in LIII; iii, pyramidal cell in LII; iv, fan cell in LII. (C) Voltage responses to light stimulation (light blue line) recorded from neurons shown in (A). Average traces (blue) are superimposed on the individual traces (gray). (D–G) The proportion of responding cells (D), excitatory postsynaptic potential (EPSP) amplitude (E), the normalized EPSP based on LIII response (F, one-way ANOVA, F3,75 = 7.675, ***p=0.0002, Bonferroni’s multiple comparison test, **p<0.01, ***p<0.001), and the input resistance (G, one-way ANOVA, F3,101 = 11.75, ***p<0.0001, Bonferroni’s multiple comparison test, *p<0.05, ***p<0.001) was examined for each cell type (error bars: mean ± standard errors). (H) Latency of EPSP onset for LEC neurons to optical activation (one-way ANOVA, F3,47 = 11.65). (I, J) Comparison of the proportion of responding cells (I) and the normalized EPSP based on LIII response (J) between medial entorhinal cortex (MEC) and LEC (error bars: mean ± standard errors; two-tailed unpaired t-test, t21 = 2.239, *p=0.0361). Figure 5—source data 1. See also Figure 5—figure supplement 1.

Figure 5—source data 1. Patch-clamp recording data in lateral entorhinal cortex.

Figure 5.

Figure 5—figure supplement 1. Representative patch-clamp recording after optical stimulation of layer Vb (LVb) fibers in lateral entorhinal cortex (LEC).

Figure 5—figure supplement 1.

(A) Semicoronal LEC slice showing expression of oChIEF-citrine in LVb neurons (green) and biocytin-filled recorded layer II/III neurons (magenta). The inset indicates the position of the higher-power image. Scale bars represent 500 μm for inset and 100 μm for high-power image. (B, C) Voltage responses to injected current steps (B) and to light stimulation (light blue line, C) recorded from neurons shown in (A) (i, ii, pyramidal cell in LVa; iii, pyramidal cell in LIII; iv, fan cell in LII). Average traces (blue) are superimposed on the individual traces (gray).