Dietary isoleucine improved flesh quality, muscle antioxidant capacity, and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish (Pelteobagrus vachelli♀ × Leiocassis longirostris♂)

Abstract

Background

Muscle is the complex and heterogeneous tissue, which comprises the primary edible part of the trunk of fish and mammals. Previous studies have shown that dietary isoleucine (Ile) exerts beneficial effects on growth in aquatic animals. However, there were limited studies regarding the benefits of Ile on fish muscle and their effects on flesh quality and muscle growth. Thus, this study was conducted to explore whether dietary Ile had affected flesh quality and muscle growth in hybrid bagrid catfish (Pelteobagrus vachelli♀ × Leiocassis longirostris♂).

Methods

A total of 630 hybrid fish, with an initial average body weight of 33.11 ± 0.09 g, were randomly allotted into seven experimental groups with three replicates each, and respectively fed seven diets with 5.0, 7.5, 10.0, 12.5, 15.0, 17.5, and 20.0 g Ile/kg diets for 8 weeks.

Results

In the present study, we demonstrated that Ile significantly: (1) increased muscle protein and lipid contents and the frequency distribution of myofibers with ≤ 20 μm and ≥ 50 μm of diameter; (2) improved pH value, shear force, cathepsin B and L activities, hydroxyproline content, resilience, cohesiveness, and decreased cooking loss, lactate content, hardness, springiness, gumminess, and chewiness; (3) decreased reactive oxygen species (ROS), malondialdehyde (MDA), and protein carbonyl (PC) contents, GCLC and Keap1 mRNA levels, and up-regulated CuZnSOD, CAT, GPX1a, GST, and Nrf2 mRNA levels; (4) up-regulated the insulin-like growth factor 1, 2 (IGF-1, IGF-2), insulin-like growth factor 1 receptor (IGF-1R), proliferating cell nuclear antigen (PCNA), Myf5, Myod, Myog, Mrf4, and MyHC mRNA levels, and decreased MSTN mRNA level; (5) increased muscle protein deposition by activating AKT-TOR-S6K1 and AKT-FOXO3a signaling pathways.

Conclusion

These results revealed that dietary Ile improved flesh quality, which might be due to increasing nutritional content, physicochemical, texture parameters, and antioxidant ability; promoting muscle growth by affecting myocytes hyperplasia and hypertrophy, and muscle protein deposition associated with protein synthesis and degradation signaling pathways. Finally, the quadratic regression analysis of chewiness, ROS, and protein contents against dietary Ile levels suggested that the optimal dietary Ile levels for hybrid bagrid catfish was estimated to be 14.19, 12.36, and 12.78 g/kg diet, corresponding to 36.59, 31.87, and 32.96 g/kg dietary protein, respectively.

Supplementary Information

The online version contains supplementary material available at 10.1186/s40104-021-00572-4.

Introduction

Recently, the global aquaculture production has reached about 82 million tons in 2018, and accounts for more than 50% of the worldwide fish food supply [1]. However, with the improvement of consumers’ health awareness, people pay more and more attention to the quality and nutritional composition of meat [2]. Thus, the aquaculture industry seems to be a growing interest in concerning about fish quality, which is influenced by a wide variety of external factors, such as feeding management, dietary composition, and amino acids ration level [3–6]. In fish, flesh quality is also regulated by nutritional factors, especially amino acids [7, 8]. As a member of branched-chain amino acids (BCAA), isoleucine (Ile) is not only the building block of protein but also the essential nutrient in aquafeeds, which plays a vital role in growth and flesh quality [9]. Our previous study have indicated that dietary Ile could improve the growth performance [10] and enhance the intestinal mucosal and physical barrier functions of hybrid bagrid catfish [11]. However, the effects of dietary Ile on flesh quality of hybrid bagrid catfish has not been extensively elucidated.

Fish flesh quality is affected by multifarious aspects including nutritional content, physicochemical, and texture parameters [12, 13]. The nutritional content was mainly reflected by muscle protein and lipid contents. Emerging shreds of evidence have shown that dietary Ile could increase muscle protein and lipid contents of grass carp (Ctenopharyngodon idella) [9] and fingerling spotted snakehead (Channa punctatus) [14]. These results provided possible evidence that dietary Ile could affect muscle nutritional content and merit further study. Additionally, physicochemical (pH value, water holding capacity, lactate, cathepsin, and hydroxyproline content) and texture parameters (hardness, springiness, cohesiveness, gumminess, chewiness, and resilience) are also the important indices for flesh quality [15]. Previous study have demonstrated that diet had a potential benefit to improve muscle flesh quality by regulating the physicochemical parameters of grass carp [9]. However, knowledge about the effects of dietary Ile on muscle texture parameters is scarce, which deserves further research. Some studies also have shown that flesh quality was negatively linked to the serious oxidative stress in carp [3, 9, 16]. Oxidative damage was primarily caused by excessive accumulation of reactive oxygen species (ROS), which could be effectively eliminated by the antioxidant system in fish [3, 8]. Our previous study have found that Ile could improve intestine function by increasing antioxidant ability via Kelch-like ECH-associated protein 1 (Keap1)- nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway in hybrid bagrid catfish [10]. However, it remains unknown whether dietary Ile could improve the quality of flesh by regulating Keap1-Nrf2 signaling pathway in hybrid bagrid catfish.

The muscle is the complex and heterogeneous tissue, which comprises the primary edible part of the trunk of fish [17]. Fish muscle growth is a dynamic process involving both the hyperplasia of myofibers and hypertrophy of existing myofibers [18], which is under the control of a series of transcription factors such as insulin-like growth factors (IGFs), proliferating cell nuclear antigen (PCNA), myogenic regulatory factors (MRFs), and myostatin (MSTN) [19, 20]. The IGFs, including IGF-1 and IGF-2, play a pivotal role in the growth and functional maturation of fish muscle [21]. Previous studies have revealed that IGFs could improve muscle growth of Japanese amberjack (Seriola quinqueradiata) [22], hybrid bagrid catfish [23], and gilthead sea bream (Sparus aurata) [24]. The MRFs, including myogenic determination (Myod), myogenic factor 5 (Myf5), myogenic regulatory factor 4 (Mrf4), and myogenin (Myog), play a critical role in myoblast proliferation and myogenic differentiation [21]. The MSTN acts as a negative regulator of muscle growth by inhibiting the myogenesis in fish [25, 26]. Despite muscle growth induced by these factors have reported in gilthead sea bream, rainbow trout (Oncorhynchus mykiss), and zebrafish (Danio rerio) [24, 27, 28]. Several studies in our laboratory also have reported that dietary threonine [23] and leucine [29] could promote the muscle growth of hybrid bagrid catfish by regulating these factors. However, there was unclear whether dietary Ile could improve muscle growth through impacting hyperplasia and hypertrophy in fish.

Fish muscle growth primarily associated with protein deposition of muscle [30]. Muscle protein deposition is the result of a balance between protein synthesis and protein degradation. The IGF-1 and IGF-2 mediate muscle growth by binding to IGF-1 receptor (IGF-1R) at the cell membrane [31, 32]. Several studies have demonstrated that IGF-1 promotes muscle growth via the IGF-1R- phosphoinositide 3-kinase (PI3K)- protein kinase B (AKT) pathway [32–34]. As a response element of the PI3K-AKT pathway, target of rapamycin (TOR) acts a central role in cell growth, development, and metabolism. The TOR could promote protein synthesis by phosphorylating ribosomal protein S6 kinase 1 (S6K1) and the eukaryotic translation initiation factor 4E (eIF4E) binding protein 1 (4E-BP1) in fish [35, 36]. Previous studies have reported that Ile could activate TOR signaling pathway and regulate the phosphorylation of S6K1 and 4E-BP1 in bovine [37] and goat [38] mammary tissue, ultimately stimulating protein synthesis. Gan et al. [9] have reported that dietary Ile increased the mRNA expressions of TOR and S6K1 in grass carp muscle. On the other hand, protein synthesis and degradation pathways are interlinked in muscle [39]. The PI3K-AKT signaling pathway also regulates protein degradation by activating the forkhead box protein O3a (FOXO3a) and subsequently inhibiting the ubiquitin-proteasome system (UPS), which mediates 80–90% protein degradation [40, 41]. The UPS associated with two key ubiquitin ligases (muscle atrophy F box, MAFBX and muscle-specific RING finger 1, MURF-1) are responsible for specific induction of the muscle protein degradation [42–44]. In previous studies, BCAA decreased amount of MURF-1 and MAFBX during proliferation and differentiation in mice myocytes [45] and protein levels of MURF-1 and MAFBX in piglet muscle [46]. It has been well reported that dietary leucine could regulate protein synthesis and degradation through targeting the corresponding signaling pathways in fish muscle [29]. However, whether dietary Ile could affect protein synthesis and protein degradation involving IGF-1-AKT-TOR and IGF-1-AKT-FOXO3a signaling pathways in fish muscle remain unknown.

Yellow catfish (Pelteobagrus fulvidraco) is a very extensive and commercially important freshwater fish species farmed in China [47]. Recently, our laboratory began to breed hybrid bagrid catfish (Pelteobagrus vachelli♀ × Leiocassis longirostris♂) in 2017, and optimal dietary threonine [23] and leucine [29] levels based on the optimal muscle growth have been investigated. This study used the same growth trial from our previous study, which have determined the effects of dietary Ile on fish growth performance [10]. The present study was performed to explore the effects of dietary Ile on flesh quality, muscle growth, protein synthesis- and degradation-related signaling pathways in hybrid bagrid catfish.

Materials and methods

Experimental diets and design

All experimental conditions and protocols were approved by the Animal Ethic Advisory Committee of Sichuan Agricultural University under permit No. DKY-S20170512. The proximate composition of the basal diet is given in the additional files (Table S1), which is the same as our previous study [10]. Fish meal, soybean meal, rapeseed meal, and corn gluten meal were used as the primary protein sources. Soybean oil and wheat flour were used as the main lipid and starch sources. Dietary crude protein and lipid were fixed at 387.8 and 71.0 g/kg diets respectively. The Ile concentrations of seven experimental diets were 5.0 (control diet), 7.5, 10.0, 12.5, 15.0, 17.5, and 20.0 g/kg diet. All diets were made iso-nitrogenous with the addition of appropriate amounts of glycine. After thoroughly mixing all diet ingredients, water was added to achieve a proper pelleting consistency and pelleted mixture through the screw Extruder (SYX62, Xiamen, China) at 2-mm diameter. The processing conditions were as follows: 100 r/min screw speed, 127 °C temperature, and 30–45 atm pressure. Floating extruded pellets were air-dried and then stored at − 20 °C in plastic bags until used.

Fish feeding and management

Hybrid bargid catfish were purchased from Rong Sen Corporation (Sichuan, China). Fish were adapted to the experimental environment for 4 weeks. A total of 630 fish of the approximate size with an average initial weight of 33.11 ± 0.09 g, were randomly allocated into 21 concrete tanks (210 L; 2.00 m × 1.00 m × 1.05 m), resulting in 30 fish each tank. During the 8-week trial, fish were fed with their respective diets to apparent satiation two times (8:00 and 18:00) under natural light and dark cycle. The water quality, temperature, dissolved oxygen, pH, nitrite, and ammonia content were recorded throughout the experiment (25 ± 2 °C, 5 mg/L, 7.0 ± 0.3, < 0.05 mg/L, < 0.5 mg/L). Continuous flowing water was maintained the rate of 1.2 L/min each tank.

Sample collection and analysis

After a fasting period of 24 h, fish in each tank were counted and weighed at the termination of the feeding trial. Then, 12 fish from each tank were randomly selected and anaesthetized in 50 mg/L benzocaine tub [48]. Muscle samples from the left sides of 6 fish in each tank were taken and kept frozen (− 20 °C) for muscle composition analysis. Meanwhile, from the right sides of the same fish, muscle samples were obtained determination of physiochemical- and antioxidant-related parameters. The muscle samples from the left sides of another 6 fish were frozen in liquid nitrogen, and then stored at − 80 °C for biochemical parameters and molecular analysis [49]. The muscle from the right sides of same 4 fish were cut into two parts of 1 cm × 1 cm × 1 cm muscle blocks using a mold, which were used for the determination of texture parameters [6]. The muscle from the right sides of another 2 fish were used for histological analysis. Although 12 fish from each treatment were sampled, 6 sampled fish from each tank were randomly selected for further analysis.

The moisture, ash, protein, and lipid contents of muscle were determined according to the previously described [50]. Muscle pH, shear force, and cooking loss were determined as previously mentioned [51]. The lactic acid content in the muscle was measured using commercially kits according to the manufacturer’s protocols (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). The muscle hydroxyproline content was determined using the method reported by Periago et al. [17]. The activities of cathepsin B and L were measured using the procedure, according to Jiang et al. [3].

The muscle samples were homogenized in 10 volumes (mg/mL) of ice-cold physiological saline solution and centrifuged at 6000×g for 20 min at 4 °C, respectively. The supernatant was collected for enzyme activity analysis. The contents of protein, MDA, PC, GSH and the activities of T-SOD, CAT, GPX, GST, GR, ASA, and AHR were measured using commercial kits [49]. The ROS contents were measured as decreased by LeBel et al. [52].

The muscle samples with an average weight 4.6 ± 0.5 g were cut into 1.0-cm thick cuboids for texture profile analysis (TPA) measurements. The hardness, cohesiveness, springiness, gumminess, chewiness, and resilience were measured using the method described by Zhang et al. [53]. Then the probe was compressed twice with a constant moving speed of 1.0 mm/s and the deformation rate was set at 75% of the specimen. Textural parameters were collected and calculated by using a force-deformation curves [16].

Histological analysis

Transverse white muscle sections (5 μm) were embedded in paraffin, and 6 fish per treatment (2 fish per tank) were stained with haematoxylin-eosin to evaluate muscle morphology [54]. Stained sections were visualized under an optic microscope and images were captured by a DS-Ril camera and software. Using Image Pro 6.0 software, myofiber number and diameter were measured for about 300 adjacent myofibers of every section each fish. Myofibers were divided into three diameter ranges (≤ 20 μm, 20–50 μm, or ≥ 50 μm) as described by de Almeida et al. [55]. Myofiber frequency was expressed as the number of myofibers in each type of diameter class relative to the total number of myofibers.

Real-time polymerase chain reaction (RT-PCR) analysis

Total RNA was extracted using RNAiso Plus (Takara, Dalian, China) and the integrity and quality of RNA were determined by 1.5% agar-gel electrophoresis and spectrophotometry, respectively. The PrimeScript™ RT reagent Kit (Takara, Dalian, China) was used to synthesize the complementary DNA (cDNA) from 1 μg RNA. The quantification of selected genes transcript levels was performed via the real-time quantitative PCR. The real-time PCR conditions were as follows: 94 °C for 5 min, followed by 40 cycles at 95 °C for 5 s, annealing at a different temperature for each gene for 10 s and 72 °C for 15 s. In order to verify the specificity of each amplicon, melting curve analysis was drawn at 65–95 °C with reading once per 0.5 °C and performed after amplification. The amplification efficiency of genes, ranging from 95% to 105%, were calculated by specific gene standard curves generated from 10-fold serial dilutions. Specific primers of hybrid bagrid catfish were exhibited in the additional files (Table S2). The relative gene expression levels were evaluated using the 2^−ΔΔCt method according to Livak et al. [56], using β-actin and 18S rRNA as the internal control genes [57].

Western blot analysis

Western blot was done to determine the muscle protein abundance following published methods [23]. Briefly, the proteins were extracted from muscle with RIPA buffer (Beyotime, Shanghai, China). The total 30 μg protein in lysates were separated by SDS-PAGE gel electrophoresis and transferred to a polyvinylidene fluoride (PVDF) membrane. The membrane was blocked with blocking buffer for 1 h at 20 °C, then incubated at 4 °C overnight with primary antibody. The primary antibodies used in this study were AKT (1:1000; total and Ser473, Cell Signaling Technology Inc., Danvers, MA, USA), TOR (1:2000; total and Ser2448, CST), S6K1 (1:2000; total and Thr421/Ser424, CST), FOXO3a (1:3000; total and Ser253, Abcam, Cambridge, MA, USA), MAFBX and MURF-1 (1:4000; Zen Biotechnology, Chengdu, Sichuan, China). The β-actin (1:1000; CST) was used as the control protein. The blots were washed five times for 3 min each, followed by 2 h incubation with appropriate secondary antibody in TBST at 25 °C. The blots were visualized using an enhanced chemoluminescence (ECL) kit (Beyotime). This experiment was repeated at least in triplicate.

Statistical analysis

All results were subjected to one-way ANOVA analysis followed by Tukey’s HSD test to evaluate the differences within treatments using the SPSS version 20.0 (SPSS Institute Inc., Chicago, USA). The trends of linear and quadratic analyses were conducted by using SAS software version 8.0 (SAS Institute Inc., North Carolina, USA). P < 0.05 were considered significant. Data were expressed as the mean ± SEM.

Results

Growth performance, muscle composition, and flesh physicochemical parameters

The present study used the same animal trail as our previous study and the results of graded levels of dietary Ile on growth parameters have been previously reported [10]. Briefly, there was no differences in survival rate and initial body weight among treatments (P > 0.05). The final body weight, feed intake, percent weight gain, specific growth rate, protein efficiency ratio, and feed efficiency were quadratically increased with dietary Ile level, and reached the maximum at 12.5 g/kg diet (P < 0.05) (Fig. S1). As shown in Tables 1 and 2, fish fed 12.5 g/kg diet increased muscle crude protein and lipid contents (P < 0.05). Fish fed 12.5 g/kg diet had lower moisture content than those fish fed other Ile-level diets (P < 0.05). However, there was no significant difference in ash value among treatments. Muscle shear force was the maximum for fish fed 12.5 g/kg diet and the minimum for fish fed 10.0 g/kg diet (P < 0.05). The pH_45min, lactate, and hydroxyproline contents were increased with Ile levels increasing up to 12.5 g/kg diet and decreased thereafter (P < 0.05). Cathepsin B and L activities were significantly depressed with Ile levels increasing up to 10.0 g/kg diet and then gradually increased (P < 0.05). However, there were no significant differences in pH_24h and cooking loss among groups.

Table 1.

Muscle composition of hybrid bagrid catfish fed experimental diets containing graded levels of Ile for 8 weeks

Items	Dietary Ile levels, g/kg							SEM	P-values
	5.0	7.5	10.0	12.5	15.0	17.5	20.0		Linear	Quadratic
Moisture	74.82a	73.90b	72.22d	71.75e	73.49c	74.02b	73.99b	0.06	<  0.01	<  0.0001
Protein	16.89a	17.71c	18.59d	18.80d	17.67c	17.34b	17.35b	0.07	0.45	<  0.0001
Lipid	5.99a	6.14b	6.82e	7.06f	6.81de	6.68cd	6.57c	0.03	<  0.0001	<  0.0001
Ash	1.63	1.62	1.64	1.63	1.65	1.62	1.62	0.01	0.93	<  0.05

Values are means ± SEM of three replicate groups with 6 fish in each group. Values within the same rows having different superscripts are significantly different (P < 0.05)

Table 2.

Muscle physiochemical parameters of hybrid bagrid catfish fed experimental diets containing graded levels of Ile for 8 weeks¹

Items2	Dietary Ile levels, g/kg							SEM	P-values
	5.0	7.5	10.0	12.5	15.0	17.5	20.0		Linear	Quadratic
pH45min	7.11a	7.15a	7.50ab	7.62b	7.60b	7.59b	7.50ab	0.05	<  0.0001	< 0.0001
pH24h	6.83	6.83	6.78	6.78	6.89	6.83	6.78	0.03	0.79	0.76
Shear force	0.26abc	0.22a	0.19a	0.32c	0.25abc	0.32bc	0.22ab	0.02	0.06	0.11
Cooking loss	20.49	18.27	19.78	19.28	19.42	17.45	19.25	0.72	0.16	0.56
Lactate	0.27c	0.24bc	0.21abc	0.16a	0.17ab	0.19ab	0.18ab	0.01	<  0.0001	<  0.01
Cathepsin B	2.04abc	1.72ab	1.63a	2.16abc	2.44c	3.34d	2.38bc	0.08	<  0.0001	0.12
Cathepsin L	0.87c	0.88c	1.05d	0.80bc	0.74b	0.72b	0.61a	0.01	<  0.0001	<  0.0001
Hydroxyproline	0.15ab	0.22bc	0.26c	0.49d	0.30c	0.13ab	0.12a	0.01	<  0.01	<  0.0001

¹ Values are mean ± SEM of three replicate groups with 6 fish in each group. Mean values with the different superscripts in the same row are significantly different (P < 0.05)

² Shear force, N/(mm·s); Cooking loss, %; Lactate content, mmol/g protein; Cathepsin B and L activities, U/g protein; Hydroxyproline concentration, μg/mg protein

Muscle cellularity and texture parameters

As shown in Table 3, dietary Ile did influence the myofibers’ frequencies in the three ranges of myofiber diameters (≤ 20 μm; 20–50 μm; ≥ 50 μm). Frequency distribution of myofibers in types of fiber diameter ≤ 20 μm and ≥ 50 μm were increased with the increasing dietary Ile levels up to 12.5 g/kg diet and then decreased (Table 4, P < 0.05). The muscle hardness, gumminess, and chewiness decreased with dietary Ile levels increasing up to 12.5 g/kg diet and increased thereafter (Table 4, P < 0.05). Fish fed 12.5 g/kg diet had higher muscle cohesiveness and springiness than fish fed other diets (Table 4, P < 0.05). The muscle resilience (Table 4) increased with dietary Ile levels increasing to 10.0 g/kg diet (P < 0.05) and plateaued thereafter (P > 0.05).

Table 3.

Muscle fibers cellularity, % frequency of hybrid bagrid catfish fed diets with graded levels of Ile for 8 weeks¹

Items2	Dietary Ile levels, g/kg							SEM	P-values
	5.0	7.5	10.0	12.5	15.0	17.5	20.0		Linear	Quadratic
d ≤ 20 μm	24.07a	26.18ab	25.12ab	33.12c	29.21bc	23.81a	24.04a	0.51	0.74	<  0.0001
20 < d < 50 μm	66.91d	63.42cd	58.69bc	48.93a	55.56b	64.31cd	66.42d	0.80	0.52	<  0.0001
d ≥ 50 μm	9.03a	10.40a	16.19bc	18.75c	15.24b	11.89ab	10.02a	0.41	<  0.05	<  0.0001

¹ Values are means ± SEM of three replicate groups with 2 fish in each group. Mean values with the different superscripts in the same row are significantly different (P < 0.05)

² d, diameter of muscle fibers

Table 4.

Texture profile analysis properties of hybrid bagrid catfish fed experimental diets containing graded levels of Ile for 8 weeks¹

Items2	Dietary Ile levels, g/kg							SEM	P-values
	5.0	7.5	10.0	12.5	15.0	17.5	20.0		Linear	Quadratic
Hardness	1690.94c	1167.61b	960.48ab	745.61a	1121.86b	1053.46ab	1040.19ab	49.51	<  0.0001	<  0.0001
Springiness	0.39ab	0.43b	0.39ab	0.44b	0.36a	0.41ab	0.39ab	0.01	0.11	0.15
Cohesiveness	0.23a	0.28bc	0.30bc	0.31c	0.27b	0.28bc	0.30bc	0.01	<  0.01	<  0.05
Gumminess	384.49b	321.81ab	289.33ab	226.26a	300.96ab	290.92ab	311.7ab	13.51	<  0.01	<  0.0001
Chewiness	155.33b	140.09ab	115.81ab	98.36a	109.10ab	116.23ab	124.19ab	6.26	<  0.01	<  0.0001
Resilience	0.13a	0.15a	0.16b	0.15b	0.15b	0.15b	0.16b	0.01	0.18	0.39

¹ Values are mean ± SEM of three replicate groups with 4 fish in each group. Mean values with the different superscripts in the same row are significantly different (P < 0.05)

² Hardness, g; Springiness, cm; Gumminess, g; Chewiness, g

Muscle antioxidant-related parameters

As indicated in Table 5, ROS, MDA, and PC contents decreased with increasing dietary Ile levels up to 12.5 g/kg diet and rose thereafter (P < 0.05). The GSH content and ASA, T-SOD, and CAT activities reached a maximum at 12.5 g/kg diet and decreased thereafter (P < 0.05). Fish fed 10.0 g/kg diet had higher AHR and GST activities than those fed other diets (P < 0.05). The GR activity elevated with increasing dietary Ile levels up to 15.0 g Ile/kg diet and then gradually decreased (P < 0.05). However, Ile did not affect GPX activity in fish muscle (P > 0.05).

Table 5.

The contents of ROS, MDA, PC, and GSH, and the activities of ASA, AHR, T-SOD, CAT, GPX, GST, and GR in the muscle of hybrid bagrid catfish fed experimental diets containing graded levels of Ile for 8 weeks

Items	Dietary Ile level, g/kg							SEM	P-values
	5.0	7.5	10.0	12.5	15.0	17.5	20.0		Linear	Quadratic
ROS,  % DCF fluorescence	100.00de	81.84c	61.68b	37.79a	36.13a	86.07cd	113.90e	0.72	<  0.0001	<  0.0001
MDA, nmol/mg protein	3.31b	2.29ab	1.65a	1.56a	1.98a	2.50ab	5.47c	0.27	<  0.01	<  0.0001
PC, nmol/mg protein	6.53c	6.06bc	4.40ab	3.60a	4.05a	4.52ab	5.01abc	0.31	<  0.0001	<  0.0001
GSH, mg/g protein	2.07a	2.76bc	3.25cd	3.51d	3.11cd	2.86bcd	2.37ab	0.12	0.15	<  0.0001
ASA, U/mg protein	143.59a	181.50b	240.67cd	284.19e	245.60d	209.21bc	176.86ab	4.19	<  0.0001	<  0.0001
AHR, U/mg protein	93.45a	95.32a	184.63e	163.67d	146.11c	114.58b	102.72ab	2.06	<  0.05	<  0.0001
T-SOD, U/mg protein	39.35ab	41.11ab	43.89b	46.63b	41.34ab	39.27ab	34.11a	1.23	<  0.01	<  0.0001
CAT, U/mg protein	1.47a	2.49b	3.33c	3.21c	3.15bc	2.99bc	2.94bc	0.15	<  0.0001	<  0.0001
GPX, U/mg protein	34.08	36.12	38.32	40.19	43.72	41.62	40.31	1.84	<  0.01	0.06
GST, U/mg protein	96.58bcd	100.03bcd	108.74d	105.23cd	93.68abc	89.56ab	82.48a	1.81	<  0.0001	<  0.0001
GR, U/mg protein	5.21a	6.94bc	8.02cd	9.76e	9.86e	9.55de	5.62ab	0.27	< 0.0001	< 0.0001

Values are mean ± SEM of three replicate groups with 6 fish in each group. Mean values with the different superscripts in the same row are significantly different (P < 0.05)

Muscle antioxidant related genes and signaling molecules expression

The mRNA levels of CuZnSOD, CAT, GPX1a, GST, and GCLC were presented in Fig. 1. The CuZnSOD, CAT, and GPX1a mRNA levels increased with dietary Ile levels increasing up to 12.5 g/kg diet and decreased thereafter (P < 0.05). The GST mRNA level increased with dietary Ile levels increasing up to 12.5 g/kg diet and plateaued thereafter (P < 0.05). The GCLC mRNA level showed a gradually decreased trend from 5.0 to 15.0 g/kg diet and then increased (P <  0.05). As shown in Fig. 2, Nrf2 mRNA level increased with dietary Ile levels increasing up to 12.5 g/kg diet and decreased thereafter and Keap1 mRNA levels exhibited the opposite pattern to Nrf2 (P < 0.05).

Fig. 1.

The mRNA levels of CuZnSOD, CAT, GST, GPX1a, and GCLC in muscle of hybrid bagrid catfish fed diets containing graded levels of Ile. Data represent means ± SEM, of six replicates. Values having different letters are significantly different (P < 0.05)

Fig. 2.

The mRNA levels of Nrf2 and Keap1 in muscle of hybrid bagrid catfish fed diets containing graded levels of Ile. Data represent means ± SEM, of six replicates. Values having different letters are significantly different (P < 0.05)

Muscle growth related genes and signaling molecules expression

The IGF1, IGF2, and IGF1R mRNA levels increased with dietary Ile levels increasing up to 12.5 g/kg diet and then decreased (Fig. 3, P < 0.05). As presented in Fig. 4, mRNA levels of PCNA, Myf5, Myod, Myog, Mrf4, and MyHC significantly increased with Ile levels increasing up to 12.5 g/kg diet and decreased thereafter. However, the MSTN mRNA level showed an opposite trend of MyHC (Fig. 4, P < 0.05).

Fig. 3.

The mRNA levels of IGF1, IGF2, and IGF1R in muscle of hybrid bagrid catfish fed diets containing graded levels of Ile. Values are means ± SEM, of three replicates, with six fish in each replicate, and different letter denotes significant difference (P < 0.05)

Fig. 4.

Effects of dietary Ile on muscle growth related gene mRNA levels in muscle of hybrid bagrid catfish fed diets containing graded levels of Ile. Values are means ± SEM, of three replicates, with six fish in each replicate, and different letter denotes significant difference (P < 0.05)

Muscle protein synthesis related genes expressions and AKT-TOR-S6K1 signaling activity

As shown in Fig. 5a, PI3K, AKT, TOR, and S6K1 mRNA levels increased with Ile levels increasing to 12.5 g/kg diet and then decreased (P < 0.05). Conversely, 4E-BP1 mRNA level showed an opposite trend of S6K1 (Fig. 5a, P < 0.05). Fish fed with 12.5 g/kg diet had elevated p-AKT protein abundance in the muscle (Fig. 5b, P < 0.05), and no significant differences was observed in total AKT protein abundance. Compared with both 5.0 and 20.0 g/kg diets, fish fed 12.5 g/kg diet exhibited a higher level of p-TOR/TOR (Fig. 5c, P < 0.05). Fish fed a diet containing 12.5 g/kg diet had higher p-S6K1 protein abundance (Fig. 5d) and did not alter total S6K1 protein abundance (P > 0.05).

Fig. 5.

Effect of dietary Ile on the PI3K-AKT-TOR signaling pathway in hybrid bagrid catfish muscle fed diets containing graded levels of Ile. (a) The mRNA levels of PI3K, AKT, TOR, S6K1, and 4E-BP1; (b) Protein abundances of p-AKT (Ser473), AKT, and ratio of p-AKT and AKT; (c) Protein abundances of p-TOR (Ser2448), TOR, and ratio of p-TOR and TOR; (d) Protein abundances of p-S6K1 (Thr421/Ser424), S6K1, and ratio of p-S6K1 and S6K1. Data represent means ± SEM, of three replicates. Values having different letters are significantly different (P < 0.05)

Muscle protein degradation related genes expressions and AKT-FOXO3a signaling activity

The FOXO3a mRNA levels of fish fed with 7.5, 10.0, and 12.5 g/kg diets were lower than those fed with other diets (Fig. 6a, P < 0.05). The MAFBX and MURF-1 mRNA levels decreased with dietary Ile levels increasing up to 12.5 g/kg diet and increased thereafter (Fig. 6a, P < 0.05). Fish fed with 12.5 g/kg diet had elevated p-FOXO3a and total FOXO3a protein abundances (Fig. 6b, P < 0.05). Compared with both 5.0 and 20.0 g/kg diets, fish fed 12.5 g/kg diet exhibited lower abundances of MURF-1 (Fig. 6c) and MAFBX (Fig. 6d).

Fig. 6.

Effects of dietary Ile on the protein degradation related signaling pathway in hybrid bagrid catfish muscle fed diets containing graded levels of Ile. (a) The mRNA levels of FOXO3a, MAFBX, and MURF-1; (b) Protein abundances of p-FOXO3a (Ser253), FOXO3a, and ratio of p-FOXO3a and FOXO3a; Protein abundances of MURF-1 (c) and MAFBX (d). Data represent means ± SEM, of three replicates. Values having different letters are significantly different (P < 0.05)

Discussion

This study used the same growth trail as our previous study, which showed that dietary Ile significantly improved the growth performance of hybrid bagrid catfish [10]. Fish growth is mainly determined by growth and protein deposition of muscle, which is the central edible portion for consumers [17]. With the enhancement of the living standard and the gradual enhancement of health awareness, healthy meat consumption would become the mainstream. Therefore, we seek to explore the potential benefits of dietary Ile on flesh quality and muscle growth.

Dietary Ile improved fish flesh quality

Fish flesh quality can be partly reflected by the nutrient composition of muscle, which is perceived by the consumers [50, 58]. Muscle protein and lipid contents represent the principal nutritional value of flesh. In the present study, fish fed 12.5 g/kg diet increased muscle crude protein and lipid contents, indicating that dietary Ile could improve the flesh nutritional quality. Previous studies have shown that dietary Ile could significantly increase muscle crude protein and fat contents in grass carp [9] and Nile tilapia (Oreochromis niloticus) [59]. Besides, flesh quality is also strongly regulated by physicochemical and texture characteristics, which are the most important aspects of attracting approach to consumers [60]. The pH value is a key factor affecting the conversion of muscle to meat [13]. The flesh pH always decreases after slaughter mainly due to the accumulation of lactic acid [61]. In the present study, dietary Ile could increase pH_45min value and reduce the lactate content of fish muscle. A similar result was reported in grass carp [9]. The increased fillet juiciness was attributed to the decreased cooking loss. In this study, dietary Ile decreased the cooking loss of fish muscle. Previous studies have shown that cathepsin activities could affect fillet juiciness and firmness in Atlantic salmon (Salmo salar) [62] and grass carp [9]. Interestingly, dietary Ile reduced cathepsin B and L activities in hybrid bagrid catfish muscle. The increased fillet firmness, which is reflected by higher muscle shear force value, has also been addressed the importance of impacting fish quality [8, 63]. In the present study, optimal dietary Ile increased shear force value of fish muscle. Johnston et al. [63] reported that the fillet firmness could partly be reflected by muscle collagen content. The hydroxyproline was the major amino acid in collagen, which mainly constituted the extracellular structure of muscle. This study found the optimal dietary Ile level significantly increased hydroxyproline concentration in fish muscle, which was in line with grass carp [9]. Altogether, these results showed a benefit that dietary Ile could improve flesh quality by influencing muscle physicochemical characteristics.

Additionally, the texture characteristics, such as hardness, springiness, cohesiveness, gumminess, chewiness, and resilience, were important freshness factors in evaluating fish flesh quality [15]. The hardness is fundamental texture index reflecting the internal binding force of muscle [64]. Consumers tend not to choose firmer fillet due to the higher flesh hardness. The chewiness is defined as the energy required masticating and determined by firmness, cohesiveness, springiness and resilience [65, 66]. The present results indicated that springiness, cohesiveness, and resilience were significantly increased but chewiness was decreased in the muscle of hybrid bagrid catfish fed with increased dietary Ile. These results were similar to those reported in grass carp [9]. Accordingly, the present study for the first time showed dietary Ile existed a significant effect on muscle texture.

Fish muscle is easy to be subjected to oxidative stress, which results in the degenerative damage of flesh quality mainly by reducing muscle pH value, water holding capacity, and tenderness of fish [67, 68]. Previous studies have indicated that flesh quality was closely related to antioxidant capacity [3, 6]. In the present study, dietary Ile significantly decreased muscle ROS, MDA, and PC contents. This results were in accordance with previous report in grass carp [9]. The ASA and AHR activities are two critical indicators used to evaluate the total scavenging capacities of O²⁻ and OH⁻, respectively [49]. The current study presented that dietary Ile increased the AHR and ASA activities in the hybrid bagrid catfish muscle. Fish antioxidant system, as an important regulator to scavenge excess O²⁻ and OH⁻, was consisted of non-enzymatic compounds and antioxidant enzymes [50]. Meanwhile, the activities of antioxidant enzymes are tightly regulated by their corresponding genes [49]. The present study indicated that dietary Ile could increase T-SOD, CAT, GPX, GST, and GR activities as well as GSH content, depending on the up-regulated of SOD, CAT, GPX1a, GST, and GCLC mRNA expressions in the hybrid bagrid catfish muscle. In fish, studies have shown that Nrf2, which was inhibited by Keap1, played a critical role in regulating antioxidant enzyme-related genes expressions [3, 6]. Dietary Ile significantly increased Nrf2 mRNA level but decreased Keap1 mRNA level in muscle, suggesting that dietary Ile might improve muscle antioxidant gene expression by regulating the Keap1-Nrf2 pathway. In addition to being associated with antioxidant capacity, previous studies have reported a strong relationship between flesh quality and muscle growth [63, 66]. However, the detailed regulation mechanism of muscle growth associated with flesh quality need further research.

Dietary Ile improved muscle growth of fish

The Ile, one of BCAA, have been reported the importance of effecting fish growth performance [9, 14, 59]. Fish growth is mainly determined by muscle growth, which constitutes the major edible sources of the fillet [6, 8]. The IGFs play an important role in promoting muscle growth by regulating myocyte hyperplasia, hypertrophy, and muscle protein accumulation [24, 28]. In fish, the IGFs, including IGF1 and IGF2, exert biological functions primarily through the binding and activation of the IGF1R, which are evolutionarily conserved genes compared to mammals [27]. In the present study, the higher mRNA levels of IGF1, IGF2, and IGF1R of hybrid bagrid catfish muscle was observed in fish fed 12.5 g/kg diet. Similar results have been reported in juvenile hybrid grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂), where Ile significantly up-regulated liver IGF-I mRNA level [69]. Meanwhile, muscle IGF1 (r = 0.821, P <  0.05) and IGF2 (r = 0.750, P = 0.052) mRNA levels were positively correlated with IGF1R mRNA level, suggesting that IGF1 and IGF2 exerts its biological functions directly via binding to IGF1R. Similarly, studies in zebrafish reported that IGF1 and IGF2 bound to the IGF1R to promote muscle growth [70]. However, the specific mechanism of whether dietary Ile improve muscle growth via affecting IGFs remains further investigation.

Dietary Ile improved myofiber hyperplasia and hypertrophy

Fish muscle growth is constituted the myocytes’ proliferation and differentiation, which are sustained by myofiber hyperplasia and hypertrophy. Myofibers hyperplasia and hypertrophy could partly be reflected by the frequency of myofibers diameter [71]. The frequency of myofiber smaller than 20 μm indicated hyperplasia, that frequency of myofiber lager than 50 μm denoted hypertrophy [59, 72]. In the current study, the percentage of myofiber with diameters smaller than 20 μm and larger than 50 μm significantly rose in fish fed dietary Ile. However, a study in Nile tilapia juveniles showed dietary Ile exhibited a non-effect in the frequency of myofiber diameter [59]. The discrepancies results may be due to the differences in fish species, feeding management, and age. All the results above suggested that dietary Ile could improve muscle growth by improving myofiber hyperplasia and hypertrophy. However, the detailed mechanisms behind the effects of dietary Ile on muscle growth need further characterization.

Fish muscle growth is controlled by several myogenesis-transcription factors [6]. The PCNA is the necessary maker for myosatellite lineages activation; subsequently, Myod and Myf5 determine myoblast proliferation, whereas Myog and Mrf4 maintain the differentiation program [31, 73]. The MyHC is necessary for myofiber hyperplasia and hypertrophy [74]. Dietary Ile significantly increased the PCNA, Myod, Myf5, Myog, Mrf4, and MyHC mRNA levels in hybrid bagrid catfish muscle, indicating that dietary Ile could improve muscle development by increasing muscle cell proliferation and differentiation. These results agreed with mice myocyte, where suitable Ile concentration in BCAA could increase the mRNA levels of Myog and Myod [45]. The MSTN performed as a negative regulator of myoblasts proliferation [75]. In this study, dietary Ile decreased the MSTN mRNA level in hybrid bagrid catfish muscle. Similar results were reported in mice myocyte [45]. Overall, dietary Ile could improve muscle cell proliferation and differentiation program by regulating PCNA, MRFs, and MSTN expression might be partly attributed to enhance muscle growth.

Dietary Ile improved muscle protein deposition

Fish muscle growth is also closely associated with muscle protein content, which is determined by the balance between protein synthesis and degradation [27, 76]. In present result, dietary Ile significantly up-regulated muscle protein content. Similar results were observed in fingerling spotted snakehead [14], grass carp [9], and Nile tilapia [59]. Previous studies have observed that IGF1-PI3K-AKT acts a positive regulator in protein synthesis in mammals [77]. Upon phosphorylation activation by PI3K, AKT would improve protein synthesis processes via the TOR pathway [78–80]. In response to stimulation by the phosphorylation of AKT, TOR phosphorylates 4E-BP1 and S6K1, which are the main regulators in the initiation of protein translation and protein synthesis, respectively [81, 82]. Despite the fact that they have been studied in mammalian muscle growth, however, the regulatory role of amino acids in fish remains poorly explored. Previous studies indicated that dietary threonine and leucine could improve muscle protein synthesis by regulating the IGF1-PI3K-AKT-TOR pathway [23, 29]. In this framework, dietary Ile increased the expressions of PI3K, AKT, TOR, and S6K1 in muscle. Whereas, the expression of 4E-BP1 showed a contrary trend to that of S6K1. Compared with control and Ile-excess diet, 12.5 g/kg diet increased phosphorylation of AKT, TOR, and S6K1. Correlation analysis showed that the protein content was positively correlated with PI3K (r = 0.607, P = 0.148), AKT (r = 0.847, P < 0.05), TOR (r = 0.786, P < 0.05), and S6K1 (r = 0.929, P < 0.01) mRNA levels and negatively correlated with 4E-BP1 (r = − 0.714, P = 0.071) mRNA level, suggesting that dietary Ile could increase protein synthesis via AKT-TOR-S6K1 signaling pathway in hybrid bagrid catfish. These results were in line with the study in bovine [37] and goat [38] mammary tissue, and MAC-T cells [83], where Ile significantly increased the AKT, mTOR, and S6K1 protein phosphorylation levels. Gan et al. [9] also reported that appropriate dietary Ile increased TOR and S6K1 expressions in grass carp muscle. These results indicated that the activation of the AKT-TOR-S6K1 signaling pathway by dietary Ile could improve fish muscle protein synthesis. However, more studies need to take a closer insight into the detailed mechanism in how dietary Ile improves fish muscle protein synthesis.

In fish muscle, protein degradation relies on three mechanisms, the autophagy-lysosome system, UPS, and calpain system [84], with the UPS as the primary way of proteolysis in nutrients deficiency [85]. The PI3K-AKT signaling pathway also play a central role in impeding protein degradation processes by inhibiting the activity of FOXO proteins inducing the nuclear export of these proteins through the nuclear pore complex [86]. The FOXO transcription factors, which induces protein degradation by increasing the expression of MURF-1 and MAFBX involved in the UPS pathway [33, 39]. Several reports in mammals have reported that Ile suppressed muscle protein degradation by inhibiting the UPS pathway [45, 87, 88]. A report in rainbow trout primary myocytes represented that dietary Ile declined the protein degradation rate [84]. In the present study, the mRNA expressions of FOXO3a, MURF-1, and MAFBX in fish muscle were declined with the dietary Ile levels up to 12.5 g/kg diet and increased thereafter. Compared with control and Ile-excess diets, 12.5 g/kg diet increased phosphorylation of FOXO3a, but it did decrease the protein abundances of MURF-1 and MAFBX, suggesting that dietary Ile inhibited the activity of FOXO3a and reduced the muscle protein degradation by the UPS pathway. Moreover, the negative correlations among the protein content and mRNA expressions of FOXO3a (r = − 0.332; P = 0.467), MURF-1 (r = − 0.633; P = 0.127), and MAFBX (r = − 0.589; P = 0.164) have been observed in this study. These results suggested that dietary Ile impeded protein degradation was partly related to AKT-FOXO3a-MURF-1/MAFBX pathway. To our best knowledge, this is the first to find that dietary Ile could increase the phosphorylation of FOXO3a, but decrease MURF-1 and MAFBX expressions in fish muscle resulting in reduced protein degradation. Collectively, the above results further sustained the conclusion that dietary Ile improved muscle growth might be partly attributed to the AKT-FOXO3a signaling in fish.

Conclusions

In conclusion, this study clarified that dietary Ile was contributed to the flesh quality of hybrid bagrid catfish. Meanwhile, the data for the first time indicated that suitable dietary Ile increased muscle mass and influenced myofiber diameter distribution, which might be through promoting protein synthesis by activating of the AKT-TOR-S6K1 signaling pathway, and inhibiting protein degradation by altering AKT-FOXO3a-MURF-1/MAFBX abundances during an 8-week growth period. Finally, based on flesh quality (chewiness and ROS) and protein deposition (protein content) related parameters, dietary Ile levels for hybrid bagrid catfish were estimated to be 14.19, 12.36, and 12.78 g/kg diet, corresponding to 36.59, 31.87, and 32.96 g/kg dietary protein, respectively.

Abbreviations

4E-BP1

eIF4E-binding protein 1

AKT

Protein kinase B

BCAA

Branched-chain amino acids

ECL

Chemoluminescence

eIF4E

Eukaryotic translation initiation factor 4E

FOXO3a

Forkhead box protein O3a

IGF-1

Insulin-like growth factor 1

IGF-2

Insulin-like growth factor 2

IGF-1R

Insulin-like growth factor 1 receptor

Ile

Isoleucine

Keap1

Kelch-like ECH-associated protein 1

MAFBX

Muscle atrophy F box

MDA

Malondialdehyde

Mrf4

Myogenic regulatory factor 4

MRFs

Myogenic regulatory factors

Nrf2

Nuclear factor erythroid 2-related factor 2

MSTN

Myostatin

MURF-1

Muscle-specific RING-finger 1

Myf5

Myogenic factor 5

Myod

Myogenic differentiation

Myog

Myogenin

PC

Protein carbonyl

PCNA

Proliferating cell nuclear antigen

PI3K

Phosphoinositide 3-kinase

PVDF

Polyvinylidene fluoride

ROS

Reactive oxygen species

RT-PCR

Real-time polymerase chain reaction

S6K1

Ribosomal protein S6 kinase 1

TOR

Target of rapamycin

TPA

Texture profile analysis

UPS

Ubiquitin-proteasome system

Authors’ contributions

Q. J, M.Y. Y, and Y. Z. conducted the trial, performed the RT-PCR, Western Blot experiments, and wrote the manuscript. J. J. and X.Q. Z. contributed to the design of the study. L. F, S.Y. Y, and W.D. J assisted in the manuscript preparation. L. Y. and Y. W. assisted with all data analysis. Y. L, D.F. C, and X.L. H assisted with the trail. The authors read and approved the final manuscript.

Funding

This study was financially supported by National Key R&D Program of China (2019YFD0900200) and the Applied Basic Research Programs of Science and Technology Commission Foundation of Sichuan Province, China (2015JY0067).

Availability of data and materials

The datasets produced and/or analyzed during the current study are available from the corresponding author on reasonable request.

Declarations

Ethics approval and consent to participate

All management conditions and experimental protocols were approved by Animal Care Advisory Committee of Sichuan Agricultural University, and conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals of Sichuan Agricultural University under permit No. DKY-S20170512.

Consent for publication

Not applicable.

Competing interests

The authors declare no conflict of interest.