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. Author manuscript; available in PMC: 2021 Apr 19.
Published in final edited form as: Cell. 2020 May 8;181(5):1046–1061.e6. doi: 10.1016/j.cell.2020.04.032

Figure 2. Electron Microscopy of SMBV Genome Release Stages.

Figure 2.

(Row I) 2D cryoelectron micrographs of particles following either no treatment (A) or post-incubation with pH 2 (E), 100°C (I), or both pH 2 + 100°C (M). (Row II) Central slices (z = 20) of cryoelectron tomograms of particles following either no treatment (B) or post-incubation with pH 2 (F), 100°C (J), or both pH 2 + 100°C (N). (Row III) Central slices of cryo-tomograms with key features highlighted. Blue, distal tips of the external fiber layer; cyan, starfish seal complex; dark gray, dsDNA; red, capsid; yellow, lipid membranes (nucleocapsid). Slices are shown for virions following either no treatment (C) or post-incubation with pH 2 (G), 100°C (K), or both pH 2 + 100°C (O). (Row IV) Scanning electron micrographs of particles in various stages of genome release following either no treatment (D) or post-incubation with pH 2 (H), 100°C (L), or both pH 2 + 100°C (P). See Videos S2, S3, S4, S5, S6, S7, S8, and S9 for videos of the tomograms and tilt series. See EMD-20745–20748 for tomogram volumes.