Figure 3.

Spontaneous withdrawal following prenatal morphine (10 and 30 mg/kg) exposure affected the body temperature and body weight of spiny mice pups. (a) Male pups exposed to 10 mg/kg morphine (N = 12) and 30 mg/kg morphine (N = 12) had significantly higher body temperatures compared to the saline-exposed controls (N = 8) (AUC [Temperature] – Saline: 192.0 $\pm$ 3.63; 10 mg/kg: 206.8 $\pm$ 1.52; 30 mg/kg: 206.3 $\pm$ 1.63). (b) Similarly, female pups exposed to 10 mg/kg morphine (N = 12) and 30 mg/kg morphine (N = 9) had significantly higher body temperatures compared to the saline-exposed controls (N = 9) (AUC [Temperature] – Saline: 190.6 $\pm$ 3.39; 10 mg/kg: 206.9 $\pm$ 1.67; 30 mg/kg: 209.1 $\pm$ 1.32). (c) Male pups exposed to 10 mg/kg morphine (N = 12) and 30 mg/kg morphine (N = 12) had significantly lower body weights compared to saline-exposed controls (N = 8) (AUC [Weight] – Saline: 64.50 $\pm$ 1.13; 10 mg/kg: 58.42 $\pm$ 1.36; 30 mg/kg: 59.17 $\pm$ 1.30). (d) In contrast, there were no significant differences in body weight in 10 mg/kg morphine (N = 12) or 30 mg/kg morphine (N = 9) exposed female pups compared to the saline-exposed controls (AUC [Weight] – Saline: 62.67 $\pm$ 1.96; 10 mg/kg: 60.04 $\pm$ 1.57; 30 mg/kg: 58.90 $\pm$ 1.48). Data are presented as means total (±SEM) during a PND 0–7 period. An asterisk symbol (∗ = saline vs. 10 mg/kg morphine) or pound symbol (# = saline vs. 30 mg/kg morphine) are used to indicate a significant difference between groups, P < 0.05.