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. 1997 Mar 1;38(1):9–16. doi: 10.1186/BF03548503

Collagenolytic Activity and its Sensitivity to Doxycycline Inhibition in Tracheal Aspirates of Horses with Chronic Obstructive Pulmonary Disease

A-L Koivunen 12, P Maisi 12,✉, Y T Konttinen 22, K Prikk 22, M Sandholm 12
PMCID: PMC8057019  PMID: 9129342

Abstract

The collagenolytic activity and its sensitivity to doxycycline inhibition in tracheal aspirates (TA) of horses with chronic obstructive pulmonary disease (COPD) was analyzed with SDS-PA gel electrophoresis (SDS-PAGE), using Type I collagen as the substrate. Both autoactive and total collagenase activities were significantly higher in TAs of horses with symptomatic COPD than in TAs of healthy horses. Doxycycline inhibition studies suggest that most of the TA collagenase is of the neutrophil type (MMP-8), but some is derived from other cells such as fibroblasts and monocyte/macrophages (MMP-1) and bacteria (bacterial collagenases). Drugs inhibiting collagenases in the respiratory tract might be worth a trial in the treatment of COPD in horses.

Keywords: proteinase, proteolysis, metalloproteinase, MMP, activity, tetracycline, equine, lung, COPD

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Acknowledgment

This study was supported by the Faculty of Veterinary Medicine, University of Helsinki, Emil Aaltonen foundation, the Academy of Finland, Helsinki University Central Hospital and Astra Research Foundation.

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