Extended Data Fig. 8. Lung or skin memory CD8⁺ T cells are preserved following microbial experience.

a-d, P14 CD8⁺ T cells were transferred into naïve mice, which were intranasally infected with PR8-gp33 influenza virus and 30 d later, mice were co-housed with pet shop mice for 45 d (a). P14 CD8⁺ T cells from spleen (b), extravascular lung (c), and bronchoalveolar lavage (BAL) fluid (d) of co-housed mice (n = 8) were enumerated and compared to infection-matched mice housed in SPF conditions (n = 8) from one experiment. e-g, OT-1 CD8⁺ T cells were transferred into naïve mice, which were intravenously infected with VSV-OVA and 30 d later, mice were co-housed with pet shop mice for 60 d (e). OT-1 CD8⁺ T cells from spleen (f) and epidermal skin (g) of co-housed mice (n = 6) were enumerated and compared to infection-matched mice housed in SPF conditions (n = 7) from one experiment. Statistical significance was determined by two-tailed Mann-Whitney U test where **p = 0.0047 (b) or **p = 0.0012 (f). Data are presented as box plots showing median, IQR, and extrema.