Figure 3.
Contribution of cell body to length variation
(A) Testing for cell-body-specific differences in flagellar length. Plot shows flagellar length before and after regeneration induced by pH shock on cells trapped in a microfluidic device. Each marker is one flagellum. Line indicates best fit to data.
(B) Testing for cell-body retention of flagellar length differences. Plot shows the difference in length between the two flagella of a cell before and after regeneration induced by pH shock.
(C) Contribution to correlated length variation from cell size variation measured by correlation between average length of the two flagella with the cell diameter in asynchronous cultures. (Green) Wild-type cells. (Pink) mat3 mutant cells having smaller average size than wild type (Umen and Goodenough, 2001). Correlation coefficients were 0.61 for wild-type cells alone and 0.70 for the combination of wild-type cells plus mat3 cells. Inset gives result of dominance analysis of flagellar length after regeneration (based on data in Figure S1E in terms of length prior to regeneration and different measures of cell size). Plot shows the incremental r2 contribution of each predictor in the model.
(D) Flagellar length variation is reduced in cells arrested in the cell cycle by growth in the dark. (Red) Growth-arrested cells. (Gray) Vegetatively growing cells.