Figure 2.

Low and high Mg induce ROS during myogenesis. After 24, 72 and 144 h of culture in DM in the presence of different extracellular concentrations of Mg, ROS accumulation was measured by 2′,7′–dichlorofluorescein (DCF) assay. Fluorescence was normalized to the cell number (a). Images of DCF fluorescence emission were acquired on living cells (10× magnification) (b). * Indicates significance with respect to 1 mM Mg (* p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001).