Figure 5.

Long-term storage at −80 °C does not compromise ASC-CS ability to limit lung injury in IAV-infected mice. Female C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. On Day6 and 8, IAV mice were sub-cutaneously injected with 2.25 mg/kg of fresh or 3 year-old (3y-o) ASC-CS (green columns) or an equivalent volume of PBS (red columns). BAL fluids were collected on Day9 to analyze for total protein levels (a), white blood cell counts (b), granzyme B (c) and myeloperoxidase levels (d), IL-6 (e), MCP-1 (f) and TNFa levels (g), and PR8 hemaggutinin (HA) levels (h). n = 4–5 per group, presented are mean +/− SEM. Lines indicate levels of analytes detected in control (saline to lung) mice. * for p < 0.05 by t-test with Welch correction (unequal variances) between CS groups and IAV group.