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Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 1996 Jun 1;37(2):139–146. doi: 10.1186/BF03548106

Atypical Aeromonas salmonicida Isolated from Diseased Turbot (Scophtalmus maximus L.)

J L Larsen 1,, K Pedersen 1
PMCID: PMC8063990  PMID: 8767692

Abstract

Larsen J.L. and K. Pedersen: Atypical Aeromonas salmonicida isolated from dis-eased torbut (Scophtalmus maximus L.). Acta vet. scand. 1996, 37, 139-146. - Atyp-ical Aeromonas salmonicida were isolated from 3 outbreaks of disease among farmed turbot (Scophthalmus maximus L.) in 3 different farms, 1 from Norway (Nl) and 2 from Denmark (DK1 and DK2). In all 3 cases, the incidence of disease and mortality was high and the main characteristic pathological finding was skin ulcers and septicaemia. The isolated bacteria were subjected to a thorough phenotypic and genotypic examination and comparison in the laboratory. All 3 isolates belonged to A. salmonicida but dis-played some very different biochemical properties. However, the 2 Danish strains, DK1 and DK2 had identical ribotypes but different from that of Nl, whereas the plasmid pro-files of DK1 and Nl were identical but different from that of DK2. These observations emphasize the need for an improvement of our understanding of the taxonomy and epi-demiology of atypical A. salmonicida.

Keywords: fish, pathogen, furunculosis

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Acknowledgments

This work was supported by The Danish Agricultural and Veterinary Research Council, grant no. 13-4710-1. The technical assistance of Mrs. M. HØjgård is gratefully appreciated.

References

  1. Austin B, Austin DA. Bacterial Fish Pathogens: Dis-ease in Farmed and Wild Fish. 2nd. Chichester, England: Ellis Horwood Ltd.; 1993. pp. 86–150. [Google Scholar]
  2. Austin DA, Mcintosh D, Austin B. Taxonomy of fish associated Aeromonas spp., with the description of Aeromonas salmonicida subsp. smithia subsp. nov. Syst. Appi. Microbiol. 1989;77:277–290. doi: 10.1016/S0723-2020(89)80026-8. [DOI] [Google Scholar]
  3. Bast L, Daly JG, DeGrandis SA, Stevenson BMW. Evaluation of profiles of Aeromonas salmonicida as epidemiological markers of furunculosis in-fections in fish. J. Fish Dis. 1988;11:133–145. doi: 10.1111/j.1365-2761.1988.tb00533.x. [DOI] [Google Scholar]
  4. Chapman PF, Cipriano RC, Teska JD. Isolation and phenotypic characterization of an oxidase-nega-tive Aeromonas salmonicida causing furunculo-sis in coho salmon (Oncorhynchus kisutch). J. Wildlife Dis. 1991;27:61–67. doi: 10.7589/0090-3558-27.1.61. [DOI] [PubMed] [Google Scholar]
  5. Dalsgaard I, Paulsen H. Atypical Aeromonas salmo-nicida isolated from diseased sand-eels, Ammod-ytes läncea (Cuvier) and Hyperoplus lanceolatus (Lesauvage). J. Fish Dis. 1986;9:361–364. doi: 10.1111/j.1365-2761.1986.tb01028.x. [DOI] [Google Scholar]
  6. Enger O. Microbial ecology of marine fish farms, with special emphasis on the fish pathogenic bac-teria Vibrio salmonicida and Aeromonas salmo-nicida. Norway: University of Bergen; 1991. [Google Scholar]
  7. Gravningen K, Dydland M. Sjukdom hos piggvar (Scophthalmus maximus L.). Norsk Veterinaer-tidsskrift. 1991;705:923–926. [Google Scholar]
  8. Husevàg B, Lunestad BT, Johannesen PJ, Enger O, Samuelsen OB. Simultaneous occurrence of Vi-brio salmonicida and antibiotic resistant bacteria in sediments at abandoned aquaculture sites. J. Fish Dis. 1991;74:631–640. doi: 10.1111/j.1365-2761.1991.tb00621.x. [DOI] [Google Scholar]
  9. Husevàg B, Lunestad BT. Presence of the fish patho-gen Aeromonas salmonicida and bacteria resist-ant to antimicrobial agents in sediments from Norwegian fish farms. Bull. Eur. Ass. Fish Pathol. 1995;15:17–19. [Google Scholar]
  10. Kado CI, Liu S-T. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 1981;745:1365–1373. doi: 10.1128/JB.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Larsen JL, Mellergaard S. Microbiological and hy-gienic problems in marine aquaculture: Furuncu-losis and vibriosis in rainbow trout (Salmo gaird-neri). Bull. Eur. Ass. Fish Pathol. 1981;7:29–32. [Google Scholar]
  12. Laxdal B. A histopathological comparison of A. sal-monicida subsp. salmonicida infection in Atlan-tic salmon (Salmo salar L.) with its ECP and a proteolytic ECP fraction. Stirling, Scotland: The Insti-tute of Aquaculture, University of Stirling; 1989. [Google Scholar]
  13. LillehaugA, Lunder T, Poppe TT. Field testing of ad-juvanted furunculosis vaccines in Atlantic sal-mon (Salmo salar L.). J. Fish Dis. 1992;15:485–596. doi: 10.1111/j.1365-2761.1992.tb00680.x. [DOI] [Google Scholar]
  14. Nielsen B, Olsen JE, Larsen JL. (1993) Plasmid pro-filing as an epidemiological marker within Aero-monas salmonicida. Dis. Aquat. Org. 1993;15:129–135. doi: 10.3354/dao015129. [DOI] [Google Scholar]
  15. Nielsen B, Olsen JE, Larsen JL. Restriction endonu-clease analysis (REA) and ribotyping of Aeromo-nas salmonicida. Dis. Aquat. Org. 1994;18:155–158. doi: 10.3354/dao018155. [DOI] [Google Scholar]
  16. Pedersen K, Kofod H, Dalsgaard I, Larsen JL. Isola-tion of an oxidase negative Aeromonas salmonic-ida from diseased turbot Scophthalmus maximus. Dis. Aquat. Org. 1994;18:149–154. doi: 10.3354/dao018149. [DOI] [Google Scholar]
  17. Pedersen K, Larsen JL. rRNA gene restriction pat-tern analysis of Vibrio anguillarum serogroup Ol. Dis. Aquat. Org. 1993;16:121–126. doi: 10.3354/dao016121. [DOI] [Google Scholar]
  18. Pedersen K, Dalsgaard I, Larsen JL. Characteriza-tion of atypical Aeromonas salmonicida isolates by ribotyping and plasmid profiling. J. appi. Bac-teriol. 1996;80:37–44. doi: 10.1111/j.1365-2672.1996.tb03187.x. [DOI] [PubMed] [Google Scholar]
  19. Popoff M. Genus III. Aeromonas. Kluyver and Van Niel 1936, 398. Bergey’s Manual of Systematic Bacteriology. 1984;I:545–548. [Google Scholar]
  20. VethaakAD Diseases of flounder (Platichthys flesus L.) in the Dutch Wadden sea, and their relation to stress factors. Neth. J. Sea Res. 1992;29:257–272. doi: 10.1016/0077-7579(92)90025-A. [DOI] [Google Scholar]
  21. Wiklund T. Atypical Aeromonas salmonicida iso-lated from ulcers of pike, Esox lucius L. J. Fish Dis. 1990;13:541–544. doi: 10.1111/j.1365-2761.1990.tb00815.x. [DOI] [Google Scholar]
  22. Wiklund T, Bylund G. A cytochrome oxidase negative bacterium (presumptively an atypical Aeromonas salmonicida) isolated from ulcerated flounders (Platichthys flesus (L.)) in the northern Baltic Sea. Bull. Eur. Ass. Fish Pathol. 1991;11:74–76. [Google Scholar]
  23. Wiklund T, Dalsgaard I. Dis. Aquat. Org. 1995. Atypical Aeromonas salmo-nicida associated with ulcerated flatfish species in the Baltic Sea and the North Sea. [Google Scholar]
  24. Wiklund T, Dalsgaard I, Eerola E, Olivier G. Charac-teristics of “atypical”, cytochromeoxidase-nega-tive Aeromonas salmonicida isolated from ulcer-ated flounders (Platichthys flesus (L.)). J. Appi. Bacteriol. 1994;76:511–520. doi: 10.1111/j.1365-2672.1994.tb01110.x. [DOI] [PubMed] [Google Scholar]

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