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. 2021 Apr 5;13(4):495. doi: 10.3390/pharmaceutics13040495

Figure 5.

Figure 5

Treatment with ADSC-EVs promoted the in vitro tube-like formation of endothelial cells. (A) Representative phase contrast images showing endothelial cells cultured on Matrigel-coated plates in medium with ADSC-EVs (0, 5, or 10 μg/mL) (scale bar: 200 pixels). (B) AngioTool64 software was used to analyse the images, and the vessels (red lines) and junctions (blue dots) are shown. (C–E) The relative total vessel length, number of junctions, and junction density were measured in the images (n = 4) using AngioTool64 software, Version 0.6a (02.18.14). * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t-test.