Table 2.
Primers of quantitative real-time PCR used in this study.
| Primer Name | Gene Name | Nucleotide Sequence (5′–3′) | Putative Function of Target Gene | PCR Product |
|---|---|---|---|---|
| 015554-F | GA20ox1(GAS) | CACGATCCCCACGATTGACA | 2OG-Fe(II) oxygenase superfamily | 144 bp |
| 015554-R | GCCCTATGCTCCACGCTATT | |||
| 007568-F | GID1c- | CGTCGCCCTGATGGTACTTT | Gibberellin receptor GID1C GN | 88 bp |
| 007568-R | AAACTCCATCCACCGGCTTT | |||
| 013954-F | GID1b | AAATGAGGCTCAATGGGGCA | Gibberellin receptor GID1B GN | 142 bp |
| 013954-R | GCGGCGACAAAAAGTGTCAT | |||
| 017149-F | CPS(GA1) | ATGACACGGCATGGGTTTCT | Ent-copalyl diphosphate synthase | 117 bp |
| 017149-R | TATCGCCCCATGAACCATCG | |||
| 016673-F | GA20ox2 | CGTGCGGTGAGGAAATCTCT | Gibberellin 20 oxidase 1 GN | 123 bp |
| 016673-R | AGCTGGCCATCTGAAGTCTG | |||
| 010912-F | SNE | AGCGGCTCTACATGGTATGC | F-box protein SNE GN | 150 bp |
| 010912-R | ACGCATCACCGAGTCTTCTC | |||
| 010100-F | GA3ox3 | ACCGGGCGATGTGAAGATTT | Gibberellin 3-beta-dioxygenase 1 GN | 85 bp |
| 010100-R | TTCCTTCCATGTTACCGGGC | |||
| 019537-F | GA2ox1 | AATGGGAGGTTCCAAAGCGT | Gibberellin 2-beta-dioxygenase 1 GN | 98 bp |
| 019537-R | TTCTCACTCAAAGGCGGTCC | |||
| 003769-F | GA20ox5 | AGTCTCATTCGCGCTGCATA | Gibberellin 20 oxidase 2 OS | 127 bp |
| 003769-R | AATAACGGTCTGCATGGGCA | |||
| 012730-F | KAO1,2 | TTTCCTACAGCAGCACCCAG | Ent-kaurenoic acid oxidase 2 GN | 99 bp |
| 012730-R | TTCAGCGTCAACCCACTCTG | |||
| 011275-F | RGA | TTTCCTACAGCAGCACCCAG | DELLA protein GAIP-B | 99 bp |
| 011275-R | TTCAGCGTCAACCCACTCTG | |||
| 002577-F | KO(G3) | GCTTTGGGACATGACCTGGA | Ent-kaurene oxidase | 103 bp |
| 002577-R | CACCTTCCATTACGTCGGCT | |||
| 019330-F | GA20ox4 | TTCGAACAGACAGGAGTGGC | Gibberellin 20 oxidase 2 OS | 101 bp |
| 019330-R | CGATCGACTCCCAAGCTGAT | |||
| 005597-F | GA2ox7 | TCCTCTGGTCGGAAGCCTTA | gibberellin 2-beta-dioxygenase 8 | 116 bp |
| 005597-R | TAAGCTTTGAGCCAGGTCGG | |||
| 004052-F | RGL3 | CGGACAACACTGACGCTTTG | DELLA protein GAI | 132 bp |
| 004052-R | TATCGAGCATGGACGGTTCG | |||
| 001863-F | GA3ox4 | ATGGGTCTAGCAGCGCATAC | gibberellin 3 oxidase 1 | 106 bp |
| 001863-R | GAACCATGATCCACCCCGTT | |||
| 009378-F | KS(GAP2) | CTCTGTGATGGCTCATGGGG | ent-kaurene synthase | 110 bp |
| 009378-R | TCACCAACACCCCATTGCTT | |||
| 025327-F | GA2ox6 | CCGGCTCGTCCTTTTGGTTA | Gibberellin 2-beta-dioxygenase 2 GN | 140 bp |
| 025327-R | AATTTCGTCGGGTCGTTGGA | |||
| 021433-F | MrUBQ1 | AAGGCGAAGATCCAAGACAA | used as the internal reference | 116 bp |
| 021433-R | GTGGAGCGTCGACTCTTTCT |
F: forward primer; R: reverse primer.