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. 2021 Apr 8;10(4):849. doi: 10.3390/cells10040849

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cholesterol analysis of BALF (A) and cholesteryl ester analysis of BALF (B). Quantification of cholesterol and the ester molecular species in 20-week control and MPS IIIA BALF were measured by LC MS/MS using an API 4000 QTrap mass spectrometer (ABSciex) coupled with a Prominence HPLC (Shimadzu). Data are expressed as concentration (pmol/mL BALF) to C17 Cholesteryl ester (Sigma C5384) and plotted as mean ± SD; two-tailed Students t-test, * indicates a significant difference, p < 0.0001 between control and MPS IIIA mice, n = 16 control and 13 MPS IIIA samples (A) and n = 15 control and 13 MPS IIIA samples (B).