Table 2.
Biocatalyzed production of lupane triterpenoids—overview.
| Microorganisms | Year | Modification of Biosynthesis | Production | Reference |
|---|---|---|---|---|
| Armillaria luteovirens Sacc | 2011 | optimization of various conditions | production of 5 | [108] |
| Inonotus obliquus | 2012 | optimization of various conditions | 69.37 mg/L of 8 | [109] |
| Inonotus obliquus | 2014 | yield increase with aqueous extract and methanol extract from birch bark | increased production of 8 | [110] |
| Saccharomyces cerevisiae | 2014 | insertion and expression regulation of genes | 5 from 0.01 to 1.92 mg L−1 OD−1 | [112] |
| Saccharomyces cerevisiae | 2015 | changes in intracellular supply of NADPH/oxygen | 1.5 and 3.2 times higher production of 5 | [113] |
| Gal80p mutant of Saccharomyces cerevisiae | 2016 | up-regulation of the expressed genes | 0.16 mg/L/OD600 of 5 | [104] |
| Saccharomyces cerevisiae | 2016 | increase in the supply of squalene | 8.23 mg/L of 9 | [114] |
| Saccharomyces cerevisiae CEN.PK BA4 | 2017 | usage of excess of ethanol | 182 mg/L of 5 | [115] |
| 2017 | [116] | |||
| Phaeodactylum tricornutum | 2019 | introduction of Lotus japonicus oxidosqualene cyclase and Medicago truncatula cytochrome P450 with native reductase | production of 8 and 9 | [117] |
| Escherichia coli and Saccharomyces cerevisiae | 2019 | usage of optimized lupeol pathway genes | production of 9 | [118] |
| Yarrowia lipolytica | 2019 | usage of glycerol as a starting material | 26.53 mg/L of 5 | [120] |
| Yarrowia lipolytica | 2019 | systematic metabolic engineering | 204.89±11.56 mg/L of triterpenoids (23.71% of 5) | [121] |
| Saccharomyces cerevisiae | 2019 | usage of RoCYP01 (CYP716A155) | yields of 5 higher than 1 g/L | [106] |
| various yeast strains | 2020 | SCRaMbLE technique | production of 5 | [122] |