Table 1.
Probes and primers for detection of A/bat/Egypt/381OP/2017 (H9N2) viral RNA (specifically designed for this study) and A/layer chicken/Bangladesh/VP02-plaque/2016 (H9N2) viral RNA.
| Primer/Probe | Sequence | Concentration | Accession Number |
|---|---|---|---|
| Detection of A/bat/Egypt/381OP/2017 (H9N2) viral RNA | |||
| H9N2-PB1-101Fv2 | tga tcc acc cta cag cca tg | 20 μM | MH376908 (Pos. 78-97) |
| H9N2-PB1-180Rv2 | ctt ttt ctg aat att gat gag tcc ta | 20 μM | MH376908 (Pos. 132-157) |
| H9N2-PB1-125FAMv2 | FAM-tgg cac agg ata tac aat gga cac cgt-BHQ1 | 5 μM | MH376908 (Pos. 102-128) |
| Detection of A/layer chicken/Bangladesh/VP02-plaque/2016 (H9N2) viral RNA | |||
| IAV-PB1_120F | cat ttg aat gga ygt caa ycc ga | 20 μM | [17] |
| IAV-PB1_271R | ctg ttd acy gtg tcc atd gtg ta | 20 μM | |
| IAV-PB1_247as_FAM | FAM-ccw gty ccy gty cca tgg ctg ta-BHQ1 | 5 μM | |