Table 1.
Gene | Forward Sequence (5′–3′) |
Reverse Sequence (3′–5′) |
Fw Position * | Rev Position * | Amplicon Length |
---|---|---|---|---|---|
YWHAZ | ACTTTTGGTACATTGTGGCTTCAA | CCGCCAGGACAAACCAGTAT | 1110–1133 | 1203–1184 | 51 bp |
β–ACTIN | ATCAAGATCATTGCTCCTCCTGA | CTGCTTGCTGATCCACATCTG | 1063–1085 | 1164–1144 | 59 bp |
CAT | TAAGACTGACCAGGGCA | CAAACCTTGGTGAGATCGAA | 788–804 | 988–969 | 165 bp |
GSS | ATGCTGTGCAGATGGACTTCAACC | TGGATGTCAAACAGACGAGCGGTA | 285–308 | 408–385 | 77 bp |
GSR | AACATCCCAACTGTGGTCTTCAGC | TTGGTAACTGCGTGATACATCGGG | 1239–1262 | 1378–1355 | 93 bp |
GPX1 | CGCAACGATGTTGCCTGGAACTTT | AGGCTCGATGTCAATGGTCTGGAA | 563–586 | 664–641 | 55 bp |
ULK1 | TCATCTTCAGCCACGCTG | CACGGTGCTGGAACATCT | 2724–2741 | 2789–2772 | 31 bp |
BECN1 | GGCTGAGAGACTGGATCAGG | CTGCGTCTGGGCATAACG | 806–825 | 932–915 | 90 bp |
HSC70 | ATTGATCTTGGCACCACCTA | ACATAGCTTGAAGTGGTTCG | 103–122 | 203–184 | 62 bp |
LAMP–2A | TGCTGGCTACCATGGGGC TG | GCAGCTGCCTGTGGAGTGAGT | 825–844 | 922–902 | 58 bp |
PHLPP1 | CCTACCTTCTCCAGTGCACT | CCAGCAGTTCCAAGTTTCCT | 3796–3815 | 3916–3897 | 82 bp |
NRF2 | CAGCGACGGAAAGAGTATGA | AAGAAACCTGGGAGTAG | 271–291 | 471–454 | 163 bp |
HIF–1 α | TGATTGCATCTCCATCTCCTACC | GACTCAAAGCGACAGATAACACG | 2205–2227 | 2381–2359 | 132 bp |
VEGF | CGAGGGCCTGGAGTGTGT | CGCATAATCTGCATGGTGATG | 335–352 | 391–371 | 19 bp |
Table 1 shows the primer sequences (forward and reverse) used to amplify all the genes, their positions and the length of amplicons. * https://blast.ncbi.nlm.nih.gov/Blast.cgi. (Accessed on 30 March 2021).