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. 2021 Apr 15;13(4):1303. doi: 10.3390/nu13041303

Table 1.

Primer details.

Gene Forward Sequence
(5′–3′)
Reverse Sequence
(3′–5′)
Fw Position * Rev Position * Amplicon Length
YWHAZ ACTTTTGGTACATTGTGGCTTCAA CCGCCAGGACAAACCAGTAT 1110–1133 1203–1184 51 bp
βACTIN ATCAAGATCATTGCTCCTCCTGA CTGCTTGCTGATCCACATCTG 1063–1085 1164–1144 59 bp
CAT TAAGACTGACCAGGGCA CAAACCTTGGTGAGATCGAA 788–804 988–969 165 bp
GSS ATGCTGTGCAGATGGACTTCAACC TGGATGTCAAACAGACGAGCGGTA 285–308 408–385 77 bp
GSR AACATCCCAACTGTGGTCTTCAGC TTGGTAACTGCGTGATACATCGGG 1239–1262 1378–1355 93 bp
GPX1 CGCAACGATGTTGCCTGGAACTTT AGGCTCGATGTCAATGGTCTGGAA 563–586 664–641 55 bp
ULK1 TCATCTTCAGCCACGCTG CACGGTGCTGGAACATCT 2724–2741 2789–2772 31 bp
BECN1 GGCTGAGAGACTGGATCAGG CTGCGTCTGGGCATAACG 806–825 932–915 90 bp
HSC70 ATTGATCTTGGCACCACCTA ACATAGCTTGAAGTGGTTCG 103–122 203–184 62 bp
LAMP–2A TGCTGGCTACCATGGGGC TG GCAGCTGCCTGTGGAGTGAGT 825–844 922–902 58 bp
PHLPP1 CCTACCTTCTCCAGTGCACT CCAGCAGTTCCAAGTTTCCT 3796–3815 3916–3897 82 bp
NRF2 CAGCGACGGAAAGAGTATGA AAGAAACCTGGGAGTAG 271–291 471–454 163 bp
HIF–1 α TGATTGCATCTCCATCTCCTACC GACTCAAAGCGACAGATAACACG 2205–2227 2381–2359 132 bp
VEGF CGAGGGCCTGGAGTGTGT CGCATAATCTGCATGGTGATG 335–352 391–371 19 bp

Table 1 shows the primer sequences (forward and reverse) used to amplify all the genes, their positions and the length of amplicons. * https://blast.ncbi.nlm.nih.gov/Blast.cgi. (Accessed on 30 March 2021).