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. 2021 Apr 17;9(4):865. doi: 10.3390/microorganisms9040865

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Schematic representation of the clustered regularly interspaced short palindromic repeats (CRISPRs) in S. Typhimurium DT4 (LT2), DT104, and DT104b. The eleven studied S. Typhimurium strains harbour two CRISPR loci, CRISPR-1 and CRISPR-2. The type I-E CRISPR/Cas system is encoded by an operon harbouring eight cas genes (blue boxed arrows) that are located upstream of CRISPR-1. Each CRISPR locus also contains a leader region (green horizontal lines). The black arrows show transcriptional orientation.