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. 2021 Apr 16;10(4):919. doi: 10.3390/cells10040919

Figure 5.

Figure 5

Effect of miRNA-145 overexpression on SMC senescence, DDR signalling and SASP. ND-SMC were transfected with premiRNA-145 or premiRNA -ve. (a) After 4 days in full growth media, cells were fixed and stained for SA-β-galactosidase (scale bar = 200 μM, n = 6). (b) IL-1α and (c) LMNB1 expression were measured by RT-PCR after 72 h (n = 7). (d) Cells were treated as (a) and stained for γH2AX (pink foci, scale bar = 100 μM, n = 6). (e) Expression levels of ATM, (f) ATR, (g) DNA-PK and (h) p21 were quantified by RT-PCR 72 h after miRNA-145 transfection (n = 7). (i) Protein secretion of IL-6, (j) IL-8 and (k) MCP-1 was measured by ELISA after 96 h (n = 11).