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. 2021 Apr 16;10(4):919. doi: 10.3390/cells10040919

Figure 7.

Figure 7

Conditioned medium from miRNA-145 overexpressing SMC induces a bystander effect in naive cells. (a) Cells were serum-starved for 72 h prior to exposure to conditioned (0.4% FCS) medium harvested from cells transfected with premiRNA-145 or premiRNA -ve for 96 h. Protein lysates were immunoblotted for (b) p38, (c) ERK, (d) Akt (all expression and phosphorylation), (e) IκB, (f) p21 expression. Phosphorylation of proteins was measured against expression of the same protein, whereas changes in expression only were measured against α-tubulin as a loading control (n = 6–9). (g) Cells were serum starved for 72hrs prior to exposure to conditioned (0.4% FCS) medium harvested from cells transfected with premiRNA-145 or premiRNA -ve for 96 h. Cell counts were performed at 48 and 96h post exposure to determine cell number or (h) were fixed and stained for presence of SA-β-galactosidase staining (scale bar = 100 μm; n = 6–9).