Table 2.
List of differentially regulated genes (DRGs) potentially linked to barrier function [23,24,25,26,27].
| Gene | Gene Description | log2 FC (co- vs. Monoculture) |
FDR p-Value |
|---|---|---|---|
| Soluble mediators | |||
| CXCL8 | Chemokine (C-X-C motif) ligand 8 | −3.7 | 1.68E-07 |
| CXCL5 | Chemokine (C-X-C motif) ligand 5 | −2.2 | 0.0009 |
| CXCL6 | Chemokine (C-X-C motif) ligand 6 | −1.9 | 0.036 |
| CXCL10 | Chemokine (C-X-C motif) ligand 10 | −1.5 | 0.0045 |
| IL1B | Interleukin 1 beta | −2.4 | 0.0003 |
| IL1A | Interleukin 1 alpha | −1.4 | 0.0021 |
| IL32 | Interleukin-32 | −1.7 | 0.0002 |
| VEGFC | Vascular endothelial growth factor C | −1.3 | 0.0016 |
| TGFB2 | Transforming growth factor beta 2 | 1.1 | 0.016 |
| BMP4 | Bone morphogenetic protein 4 | 1.5 | 0.0048 |
| BMP6 | Bone morphogenetic protein 6 | 1.4 | 0.0021 |
| Junctional Proteins | |||
| CLDN1 | Claudin-1 | −2.0 | 4.69E-05 |
| OCCL | Occludin | −1.1 | 0.034 |
| ECM Proteins | |||
| ADAMTS6 | ADAM metallopeptidase with thrombospondin type 1 motif 6 | −1.3 | 0.0008 |
| LAMB3 | Laminin beta 3 | −1.9 | 0.0008 |
| SERPINE1 | Serpin peptidase inhibitor, calde E (nexin, plasminogen activator inhibitor, type 1) | −1.6 | 0.0012 |
| PLAU | Plasminogen activator, urokinase | −1.5 | 0.0019 |
| LTBP1 | Latent transforming growth factor beta binding protein 1 | −1.1 | 0.0022 |
| EFEMP1 | EGF containing fibulin-like ECM protein1 | 0.86 | 0.0125 |
| SERPINA3 | Serpin peptidase inhibitor, calde A (alpha-1 antiproteinase, antitrypsin) | 1.6 | 0.0252 |
| BGN | Biglycan | 1.5 | 0.0348 |
| Cell surface proteins | |||
| JAG1 | Jagged 1 | −1.2 | 0.020 |
| LPAR1 | Lysophosphatidic acid receptor 1 | −1.3 | 0.0009 |
| LEPR | Leptin receptor | 1.1 | 0.0092 |
Log2 Fold Changes (log2FC) of gene expression levels in co-cultured vs. mono-cultured endothelial cells and adjusted p-values are depicted in the third and fourth column, respectively.